Abstract
Hybrid aspen is a major source of fiber in the north-central United States. One major problem for utilizing genetically improved aspen, and a variety of other woody species, is the difficulty in rooting hardwood cuttings. The objective of this project was to construct and confirm the function of genetic transformation vectors with two rooting genes ( iaaM from Agrobacterium tumefaciens and rolB from A. rhizogenes ) using three promoters (CaMV35S, soybean heat shock-inducible and poplar wound-inducible promoters). The gene constructs can be used to transform aspen for improvement of rooting hardwood cuttings and to elucidate rooting mechanisms. Each of these six gene constructs was inserted contiguously upstream from the promoter-less-glucuronidase (GUS) gene in the plasmid pBI 101. The engineered plasmids were transformed into A. tumefaciens strain LBA 4404 by electroporation. The functions of the genes were confirmed by transforming Nicotiana tabacum with these constructs. In plants transformed with iaaM and rolB under the CaMV35S promoter, the GUS gene expressed constitutively. These plants were also morphologically abnormal, with callus (and sometimes root) formation on stem tissue. The plants transformed with heat shock and Win6 constructs had no root formation and little or no GUS expression, without induction. After induction with heat shock (42°C for 1 hr per day for 7 days) or wounding (wounding the plants daily for 7 days), root formation occurred and GUS expression increased significantly with each gene, respectively. No roots developed in the non-transformed control shoots. The transformed plants with the regulatory promoters appeared morphologically normal. These constructs are currently being tested in hybrid aspen.
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Claims 9
Claim · model-written summary
CaMV35S-driven iaaM and rolB transformants expressed GUS constitutively.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0t0 - Material
- plants transformed with iaaM and rolB under the CaMV35S promotere0
- Gene
- GUSe0
- Outcome
- GUS gene expressed constitutivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In plants transformed with iaaM and rolB under the CaMV35S promoter, the GUS gene expressed constitutively.”
t0“Nicotiana tabacum”
Claim · model-written summary
CaMV35S-driven iaaM and rolB transformants were morphologically abnormal with callus and occasional roots on stems.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Organ / stage
- stem tissuee0
- Outcome
- morphologically abnormal, with callus (and sometimes root) formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants were also morphologically abnormal, with callus (and sometimes root) formation on stem tissue.”
t0“Nicotiana tabacum”
Claim · model-written summary
Without induction, heat shock and Win6 transformants formed no roots.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Material
- plants transformed with heat shock and Win6 constructse0
- Outcome
- no root formatione0
- Qualifier
- without inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plants transformed with heat shock and Win6 constructs had no root formation and little or no GUS expression, without induction.”
t0“Nicotiana tabacum”
Claim · model-written summary
Without induction, heat shock and Win6 transformants had little or no GUS expression.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Gene
- GUSe0
- Outcome
- little or no GUS expressione0
- Qualifier
- without inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plants transformed with heat shock and Win6 constructs had no root formation and little or no GUS expression, without induction.”
t0“Nicotiana tabacum”
Claim · model-written summary
Heat shock induction caused root formation in heat-shock construct transformants.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- heat shock (42°C for 1 hr per day for 7 days)e0
- Outcome
- root formation occurrede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After induction with heat shock (42°C for 1 hr per day for 7 days) or wounding (wounding the plants daily for 7 days), root formation occurred and GUS expression increased significantly with each gene, respectively.”
t0“Nicotiana tabacum”
Claim · model-written summary
Wounding induction caused root formation in Win6 construct transformants.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- wounding (wounding the plants daily for 7 days)e0
- Outcome
- root formation occurrede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After induction with heat shock (42°C for 1 hr per day for 7 days) or wounding (wounding the plants daily for 7 days), root formation occurred and GUS expression increased significantly with each gene, respectively.”
t0“Nicotiana tabacum”
Claim · model-written summary
Induction significantly increased GUS expression in each gene construct.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- inductione0
- Gene
- GUSe0
- Outcome
- GUS expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After induction with heat shock (42°C for 1 hr per day for 7 days) or wounding (wounding the plants daily for 7 days), root formation occurred and GUS expression increased significantly with each gene, respectively.”
t0“Nicotiana tabacum”
Claim · model-written summary
Non-transformed control shoots developed no roots.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Material
- non-transformed control shootse0
- Outcome
- No roots developede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No roots developed in the non-transformed control shoots.”
t0“Nicotiana tabacum”
Claim · model-written summary
Plants transformed with regulatory promoters appeared morphologically normal.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Outcome
- morphologically normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transformed plants with the regulatory promoters appeared morphologically normal.”
t0“Nicotiana tabacum”
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.