Abstract
The effect of FeSO4.7H2O, Fe2(SO4)3.9H2O, disodium salt of ethylene-diaminotetraacetic acid, dihydrate (EDTA) and N-(2-acetamido) iminodiacetic acid (ADA) and their combinations on the androgenesis was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.). Simultaneously the reversibility and irreversibility of the morphogenic process leading to the conversion of the pollen embryoid into complete plant was followed. Complete plants developed in anthers on media with trivalent iron, chelated trivalent iron, chelated bivalent iron, bivalent iron in the presence of ADA and of media with EDTA. The number of androgenic plants in anthers increased in the following order: Fe3+ < Fe3+ EDTA ≦ ≦ EDTA < Fe2+ EDTA. The marked brown colour of cultured anthers was due to the presence of trivalent iron in the medium. The androgenic development was most rapid on the medium containing only trivalent iron, slower on media with chelated iron and slowest on medium with EDTA. The viability of cultures with complete plants decreased in the reverse order. No complete plants grew on media without trivalent iron and without EDTA and on media containing only bivalent iron whereas globular embryoids arose and developed continuously on these media. The anthers reacted in the same way on both complete and minimal media. Isolated embryoids formed complete plants in corresponding variants on complete media only. The development of pollen embryoids into complete plants was stopped by the transfer of globular and torpedo-shaped embryoids from medium with EDTA to the medium without EDTA. Isolated greenish cotyledonar embryoids continued to grow even on the medium without EDTA.
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Claims 12
Claim · model-written summary
Fe2+ EDTA gave the largest number of androgenic plants in anthers, followed by EDTA and Fe3+.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Fe2+ EDTAe2
- Outcome
- number of androgenic plants in antherse2
- Direction
- increasede2
- Comparator
- Fe3+e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e2“The number of androgenic plants in anthers increased in the following order: Fe3+ < Fe3+ EDTA ≦ ≦ EDTA < Fe2+ EDTA.”
Claim · model-written summary
Androgenic development was most rapid on medium containing only trivalent iron, slower on chelated iron and slowest on EDTA medium.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- medium containing only trivalent irone4
- Outcome
- androgenic developmente4
- Direction
- most rapide4
- Comparator
- medium with EDTAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e4“The androgenic development was most rapid on the medium containing only trivalent iron, slower on media with chelated iron and slowest on medium with EDTA”
Claim · model-written summary
Viability of cultures with complete plants decreased in the reverse order of androgenic rate.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- viability of cultures with complete plantse5
- Direction
- decreasede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e5“The viability of cultures with complete plants decreased in the reverse order”
Claim · model-written summary
No complete plants grew on media containing only bivalent iron.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- media containing only bivalent irone6
- Outcome
- complete plants grewe6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e6“No complete plants grew on media without trivalent iron and without EDTA and on media containing only bivalent iron whereas globular embryoids arose and developed continuously on these media”
Claim · model-written summary
No complete plants grew on media without trivalent iron and without EDTA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- media without trivalent iron and without EDTAe6
- Outcome
- complete plants grewe6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e6“No complete plants grew on media without trivalent iron and without EDTA and on media containing only bivalent iron whereas globular embryoids arose and developed continuously on these media”
Claim · model-written summary
Globular embryoids arose and developed continuously on media containing only bivalent iron.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- globular embryoids arose and developed continuouslye6
- Qualifier
- on these mediae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e6“No complete plants grew on media without trivalent iron and without EDTA and on media containing only bivalent iron whereas globular embryoids arose and developed continuously on these media”
Claim · model-written summary
Anthers reacted in the same way on complete and minimal media.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- reacted in the same waye7
- Qualifier
- on both complete and minimal mediae7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e7“The anthers reacted in the same way on both complete and minimal media”
Claim · model-written summary
Transfer of globular and torpedo-shaped embryoids from EDTA medium to medium without EDTA stopped the development of pollen embryoids into complete plants.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- transfer of globular and torpedo-shaped embryoids from medium with EDTA to the medium without EDTAe6
- Outcome
- development of pollen embryoids into complete plantse6
- Direction
- stoppede6
- Comparator
- medium with EDTAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e6“The development of pollen embryoids into complete plants was stopped by the transfer of globular and torpedo-shaped embryoids from medium with EDTA to the medium without EDTA”
Claim · model-written summary
Isolated greenish cotyledonar embryoids continued to grow even on medium without EDTA.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- greenish cotyledonar embryoidse6
- Outcome
- continued to growe6
- Qualifier
- even on the medium without EDTAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e6“Isolated greenish cotyledonar embryoids continued to grow even on the medium without EDTA”
Claim · model-written summary
Isolated embryoids formed complete plants only on complete media.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- Isolated embryoidse6
- Outcome
- formed complete plantse6
- Qualifier
- on complete media onlye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e6“Isolated embryoids formed complete plants in corresponding variants on complete media only”
Claim · model-written summary
Complete plants developed in anthers on media with trivalent iron, chelated iron, bivalent iron with ADA, and EDTA.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- Complete plants developed in antherse1
- Qualifier
- media with trivalent iron, chelated trivalent iron, chelated bivalent iron, bivalent iron in the presence of ADA and of media with EDTAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e1“Complete plants developed in anthers on media with trivalent iron, chelated trivalent iron, chelated bivalent iron, bivalent iron in the presence of ADA and of media with EDTA”
Claim · model-written summary
The marked brown colour of cultured anthers was due to trivalent iron in the medium.
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- trivalent irone3
- Outcome
- marked brown colour of cultured antherse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was studiedin vitro in tobacco (cv. White Burley) and datura (Datura innoxia Mill.)”
e3“The marked brown colour of cultured anthers was due to the presence of trivalent iron in the medium”
The same questions in other papers
in vitro · growth · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 32 in the opposite direction
- positive
- positive
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.