Abstract
Tobacco leaf explants can produce both shoots and roots depending on the phytohormones in the medium. These arise directly via meristemoids (meristematic centers), which form distinct primordia and then organs. In this study it was found that shoot primordia arose from the palisade mesophyll cells at the adaxial surface, while root primordia arose from the rib parenchyma cells, near the existing vascular bundles. In studies on competency and determination, it was found that the tobacco leaf explants required 46 days in culture on a shoot-inducing medium (SIM) to become determined for shoot formation, while the explants were competent for rooting at excision and needed only 1 day on the root-inducing medium (RIM) to become determined for root formation. Transfer of explants from SIM or RIM to basal medium (BM without phytohormones) and vice versa supported the above findings. Transfer of explants from SIM to RIM and vice versa, delayed the timing of root and shoot formation, but not the position in the explant from which the organs arose. On transfer from SIM to RIM or vice versa, meristemoids that were already determined for shoot or root formation continued to develop, while those not yet determined were inhibited and (or) reverted to parenchymatous tissue. Thus under our culture conditions meristemoids in tobacco leaf explants are not plastic.Key words: competence, determination, meristemoid plasticity, organogenesis, tobacco.
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Claims 10
Claim · model-written summary
Shoot primordia arose from palisade mesophyll cells at the adaxial surface.
- Taxon
- tobacco
Nicotiana tabacumt0 - Organ / stage
- palisade mesophyll cellse0
- Outcome
- shoot primordia arose from the palisade mesophyll cells at the adaxial surfacee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot primordia arose from the palisade mesophyll cells at the adaxial surface”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Root primordia arose from rib parenchyma cells near vascular bundles.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- rib parenchyma cellse0
- Outcome
- root primordia arose from the rib parenchyma cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root primordia arose from the rib parenchyma cells, near the existing vascular bundles”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Tobacco explants required 46 days on SIM to become determined for shoots.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- shoot-inducing medium (SIM)e0
- Outcome
- determined for shoot formatione0
- Value
- 46 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the tobacco leaf explants required 46 days in culture on a shoot-inducing medium (SIM) to become determined for shoot formation”
Claim · model-written summary
Tobacco explants were competent for rooting at excision.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- competent for rooting at excisione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the explants were competent for rooting at excision”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Tobacco explants needed one day on RIM for root determination.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- root-inducing medium (RIM)e0
- Outcome
- determined for root formatione0
- Value
- 1 daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“needed only 1 day on the root-inducing medium (RIM) to become determined for root formation”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Switching between SIM and RIM delayed root and shoot formation.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Transfer of explants from SIM to RIM and vice versae0
- Outcome
- timing of root and shoot formatione0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transfer of explants from SIM to RIM and vice versa, delayed the timing of root and shoot formation, but not the position in the explant from which the organs arose”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Switching between SIM and RIM did not alter where organs arose.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Transfer of explants from SIM to RIM and vice versae0
- Outcome
- position in the explant from which the organs arosee0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transfer of explants from SIM to RIM and vice versa, delayed the timing of root and shoot formation, but not the position in the explant from which the organs arose”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Determined meristemoids continued developing after medium switch.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- meristemoidse0
- Outcome
- continued to develope0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“meristemoids that were already determined for shoot or root formation continued to develop”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
Undetermined meristemoids were inhibited or reverted to parenchyma after medium switch.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- inhibited and (or) reverted to parenchymatous tissuee0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those not yet determined were inhibited and (or) reverted to parenchymatous tissue”
t0“tobacco organogenesis in vitro”
Claim · model-written summary
The authors conclude that meristemoids are not plastic under their culture conditions.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- meristemoids in tobacco leaf explants are not plastice0
- Qualifier
- under our culture conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thus under our culture conditions meristemoids in tobacco leaf explants are not plastic”
The same questions in other papers
in vitro · growth · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 32 in the opposite direction
- positive
- positive
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