Abstract
14 C‐salicylic acid (SA) was used to monitor SA metabolism and its regulation in tobacco cell suspension culture. Two SA concentrations (20 μM and 200 μM) were used for comparison. SA was quickly taken up in both treatments, and the 200 μM‐treated cells absorbed approximately 15 times that of 20 μM‐treated cells within 5 min. More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 μM and 20 μM SA, respectively. SA excretion was significantly inhibited by EGTA and the inhibition could be reversed by the addition of exogenous Ca 2+ to the culture medium in the 200 μM SA treatment. However, EGTA had little or no effect on SA excretion in the 20 μM SA treatment. The data suggest that tobacco suspension‐cultured cells may contain both Ca 2+ ‐dependent and Ca 2+ ‐independent pathways for SA excretion. Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 μM but not in the 20 μM SA treatment. These data support the existence of alternative SA excretion pathways in tobacco suspension‐cultured cells. Tobacco cells may use both Ca 2+ ‐dependent and Ca 2+ ‐independent excretion pathways to cope with different intracellular SA status, and the pathway influenced by EGTA, reduced glutathione, staurosporine, and cycloheximide is activated by SA at 200 μM, but not at 20 μM.
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Claims 11
Claim · model-written summary
Tobacco cells quickly took up salicylic acid at both tested concentrations.
- Taxon
- tobacco
Nicotiana tabacumt0 - Outcome
- SA was quickly taken upe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA was quickly taken up in both treatments”
t0“tobacco”
Claim · model-written summary
Cells treated with 200 μM salicylic acid absorbed about 15 times the amount absorbed at 20 μM within five minutes.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- 200 μMe0
- Outcome
- absorbede0
- Direction
- approximately 15 timese0
- Value
- approximately 15 timese0
- Comparator
- 20 μMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 200 μM‐treated cells absorbed approximately 15 times that of 20 μM‐treated cells within 5 min”
t0“tobacco”
Claim · model-written summary
At 200 μM, more than 85% of absorbed salicylic acid was excreted within five hours.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- 200 μMe0
- Outcome
- absorbed SA were excreted in free forme0
- Value
- More than 85%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 μM and 20 μM SA, respectively”
t0“tobacco”
Claim · model-written summary
At 20 μM, 50% of absorbed salicylic acid was excreted within five hours.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- 20 μMe0
- Outcome
- absorbed SA were excreted in free forme0
- Value
- 50%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 μM and 20 μM SA, respectively”
t0“tobacco”
Claim · model-written summary
EGTA significantly inhibited salicylic acid excretion at 200 μM.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- EGTAe0
- Outcome
- SA excretione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA excretion was significantly inhibited by EGTA”
t0“tobacco”
Claim · model-written summary
Exogenous calcium reversed EGTA inhibition at 200 μM salicylic acid.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- addition of exogenous Ca 2+e0
- Outcome
- inhibitione0
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibition could be reversed by the addition of exogenous Ca 2+ to the culture medium in the 200 μM SA treatment”
t0“tobacco”
Claim · model-written summary
EGTA had little or no effect on salicylic acid excretion at 20 μM.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- EGTAe0
- Outcome
- SA excretione0
- Direction
- little or no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGTA had little or no effect on SA excretion in the 20 μM SA treatment”
t0“tobacco”
Claim · model-written summary
Reduced glutathione blocked salicylic acid excretion at 200 μM but not 20 μM.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Reduced glutathionee0
- Outcome
- intracellular SA excretione0
- Direction
- blockede0
- Comparator
- 20 μMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 μM but not in the 20 μM SA treatment”
t0“tobacco”
Claim · model-written summary
Staurosporine blocked salicylic acid excretion at 200 μM but not 20 μM.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- staurosporinee0
- Outcome
- intracellular SA excretione0
- Direction
- blockede0
- Comparator
- 20 μMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 μM but not in the 20 μM SA treatment”
t0“tobacco”
Claim · model-written summary
Cycloheximide blocked salicylic acid excretion at 200 μM but not 20 μM.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- cycloheximidee0
- Outcome
- intracellular SA excretione0
- Direction
- blockede0
- Comparator
- 20 μMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 μM but not in the 20 μM SA treatment”
t0“tobacco”
Claim · model-written summary
Tobacco cells may have calcium-dependent and calcium-independent salicylic acid excretion pathways.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- Ca 2+ ‐dependent and Ca 2+ ‐independent pathways for SA excretione0
- Qualifier
- suspension‐cultured cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco suspension‐cultured cells may contain both Ca 2+ ‐dependent and Ca 2+ ‐independent pathways for SA excretion”
The same questions in other papers
cycloheximide · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
8 papers more in this group
See all 12 papers →staurosporine · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
calcium(2+) · disease · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
glutathione · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- no effect
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.