Abstract
14C-salicylic acid (SA) was used to monitor SA metabolism and its regulation in tobacco cell suspension culture. Two SA concentrations (20 microM and 200 microM) were used for comparison. SA was quickly taken up in both treatments, and the 200 microM-treated cells absorbed approximately 15 times that of 20 microM-treated cells within 5 min. More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 microM and 20 microM SA, respectively. SA excretion was significantly inhibited by EGTA and the inhibition could be reversed by the addition of exogenous Ca2+ to the culture medium in the 200 microM SA treatment. However, EGTA had little or no effect on SA excretion in the 20 microM SA treatment. The data suggest that tobacco suspension-cultured cells may contain both Ca2+-dependent and Ca2+-independent pathways for SA excretion. Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 microM but not in the 20 microM SA treatment. These data support the existence of alternative SA excretion pathways in tobacco suspension-cultured cells. Tobacco cells may use both Ca2+-dependent and Ca2+-independent excretion pathways to cope with different intracellular SA status, and the pathway influenced by EGTA, reduced glutathione, staurosporine, and cycloheximide is activated by SA at 200 microM, but not at 20 microM.
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Claims 10
Claim · model-written summary
Tobacco cells rapidly took up salicylic acid at both concentrations.
- Taxon
- tobacco
Nicotiana tabacume0 - Outcome
- SA was quickly taken upe1
- Qualifier
- both treatmentse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“SA was quickly taken up in both treatments”
Claim · model-written summary
At 200 microM, tobacco cells absorbed approximately 15 times as much salicylic acid as at 20 microM within 5 min.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 200 microMe1
- Outcome
- absorbede1
- Value
- approximately 15 timese1
- Qualifier
- within 5 mine1
- Comparator
- 20 microM-treated cellse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“the 200 microM-treated cells absorbed approximately 15 times that of 20 microM-treated cells within 5 min”
Claim · model-written summary
Tobacco cells treated with 200 microM SA excreted more than 85% of absorbed SA within 5 h.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 200 microMe1
- Outcome
- absorbed SA were excretede1
- Value
- More than 85%e1
- Qualifier
- within 5 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 microM and 20 microM SA, respectively”
Claim · model-written summary
Tobacco cells treated with 20 microM SA excreted 50% of absorbed SA within 5 h.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 20 microMe1
- Outcome
- absorbed SA were excretede1
- Value
- 50%e1
- Qualifier
- within 5 he1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“More than 85% and 50% of the absorbed SA were excreted in free form to the culture medium within 5 h from cells treated with 200 microM and 20 microM SA, respectively”
Claim · model-written summary
EGTA significantly inhibited SA excretion at 200 microM SA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- EGTAe1
- Outcome
- SA excretione1
- Direction
- inhibitede1
- Qualifier
- 200 microM SA treatmente2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“SA excretion was significantly inhibited by EGTA”
e2“in the 200 microM SA treatment”
Claim · model-written summary
Exogenous Ca2+ reversed EGTA inhibition of SA excretion at 200 microM SA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- addition of exogenous Ca2+e1
- Outcome
- inhibitione1
- Direction
- reversede1
- Qualifier
- 200 microM SA treatmente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“the inhibition could be reversed by the addition of exogenous Ca2+ to the culture medium in the 200 microM SA treatment”
Claim · model-written summary
EGTA had little or no effect on SA excretion at 20 microM SA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- EGTAe1
- Outcome
- SA excretione1
- Direction
- little or no effecte1
- Qualifier
- 20 microM SA treatmente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“EGTA had little or no effect on SA excretion in the 20 microM SA treatment”
Claim · model-written summary
Reduced glutathione blocked SA excretion at 200 microM but not 20 microM SA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Reduced glutathionee1
- Outcome
- intracellular SA excretione1
- Direction
- blockede1
- Qualifier
- 200 microMe1
- Comparator
- 20 microM SA treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 microM but not in the 20 microM SA treatment”
Claim · model-written summary
Staurosporine blocked SA excretion at 200 microM but not 20 microM SA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- staurosporinee1
- Outcome
- intracellular SA excretione1
- Direction
- blockede1
- Qualifier
- 200 microMe1
- Comparator
- 20 microM SA treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 microM but not in the 20 microM SA treatment”
Claim · model-written summary
Cycloheximide blocked SA excretion at 200 microM but not 20 microM SA.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cycloheximidee1
- Outcome
- intracellular SA excretione1
- Direction
- blockede1
- Qualifier
- 200 microMe1
- Comparator
- 20 microM SA treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cell suspension culture”
e1“Reduced glutathione (an active oxygen species scavenger), staurosporine (a protein kinase inhibitor), and cycloheximide (an inhibitor of de novo protein synthesis) also blocked intracellular SA excretion to the culture medium in the 200 microM but not in the 20 microM SA treatment”
The same questions in other papers
cycloheximide · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
8 papers more in this group
See all 12 papers →staurosporine · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
calcium(2+) · disease · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
glutathione · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- no effect
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.