Abstract
Rice (Oryza sativa L.) is a staple food for billions of people globally. Rice cultivar RD43 has been recognized for its health benefits but has faced declining productivity due to climate change. Plant tissue culture serves as a powerful tool for studying and improving rice cultivars, yet a standardized protocol for rice cv. RD43 is lacking. This study aims to establish an efficient plant tissue culture protocol for rice cv. RD43 by evaluating concentrations of plant growth regulators for callus induction, proliferation, and regeneration. Callus induction was most effective with 4.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D), while callus proliferation was effective with 2.0 mg/L of 2,4-D. Furthermore, 2.0 mg/L of 6-benzyladenine (BA) yielded the highest plant regeneration, achieving a 50% regeneration rate and producing 9.60 shoots per callus. These findings lay the groundwork for a robust tissue culture protocol for rice cv. RD43 as a means for advanced breeding studies and contributing to global food security amid climate change challenges.
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Claims 4
Claim · model-written summary
Callus induction was most effective at 4.0 mg/L 2,4-D.
- Taxon
- Rice
Oryza sativat0 - Material
- RD43t0
- Treatment
- 4.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D)e0
- Outcome
- Callus inductione0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus induction was most effective with 4.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D)”
t0“Rice Cultivar RD43”
Claim · model-written summary
Callus proliferation was effective at 2.0 mg/L 2,4-D.
- Taxon
- RiceOryza sativat0
- Material
- RD43t0
- Treatment
- 2.0 mg/L of 2,4-De0
- Outcome
- callus proliferatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus proliferation was effective with 2.0 mg/L of 2,4-D”
t0“Rice Cultivar RD43”
Claim · model-written summary
BA at 2.0 mg/L produced the highest regeneration rate, 50%.
- Taxon
- RiceOryza sativat0
- Material
- RD43t0
- Treatment
- 2.0 mg/L of 6-benzyladenine (BA)e0
- Outcome
- regeneration ratee0
- Direction
- higheste0
- Value
- 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.0 mg/L of 6-benzyladenine (BA) yielded the highest plant regeneration, achieving a 50% regeneration rate”
t0“Rice Cultivar RD43”
Claim · model-written summary
BA at 2.0 mg/L produced 9.60 shoots per callus.
- Taxon
- RiceOryza sativat0
- Material
- RD43t0
- Treatment
- 2.0 mg/L of 6-benzyladenine (BA)e0
- Outcome
- shoots per calluse0
- Value
- 9.60 shoots per calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2.0 mg/L of 6-benzyladenine (BA) yielded the highest plant regeneration, achieving a 50% regeneration rate and producing 9.60 shoots per callus”
t0“Rice Cultivar RD43”
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See all 107 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.