Abstract
Drought is the stressor with a significant adverse impact on the yield stability of tea plants. HD-ZIP III transcription factors (TFs) play important regulatory roles in plant growth, development, and stress responses. However, whether and how HD-ZIP III TFs are involved in drought response and tolerance in tea plants remains unclear. Here, we identified seven HD-ZIP III genes (CsHDZ3-1 to CsHDZ3-7) in tea plant genome. The evolutionary analysis demonstrated that CsHDZ3 members were subjected to purify selection. Subcellular localization analysis revealed that all seven CsHDZ3s located in the nucleus. Yeast self-activation and dual-luciferase reporter assays demonstrated that CsHDZ3-1 to CsHDZ3-4 have trans-activation ability whereas CsHDZ3-5 to CsHDZ3-7 served as transcriptional inhibitors. The qRT-PCR assay showed that all seven CsHDZ3 genes could respond to simulated natural drought stress and polyethylene glycol treatment. Further assays verified that all CsHDZ3 genes can be cleaved by csn-miR166. Overexpression of csn-miR166 inhibited the expression of seven CsHDZ3 genes and weakened drought tolerance of tea leaves. In contrast, suppression of csn-miR166 promoted the expression of seven CsHDZ3 genes and enhanced drought tolerance of tea leaves. These findings established the foundation for further understanding the mechanism of CsHDZ3-miR166 modules' participation in drought responses and tolerance.
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Claims 12
Claim · model-written summary
Seven HD-ZIP III genes were identified in the tea plant genome.
Verbatim
e0“Here, we identified seven HD-ZIP III genes (CsHDZ3-1 to CsHDZ3-7) in tea plant genome.”
Claim · model-written summary
CsHDZ3 members showed purifying selection.
Verbatim
e0“The evolutionary analysis demonstrated that CsHDZ3 members were subjected to purify selection.”
e1“tea plant genome”
Claim · model-written summary
All seven CsHDZ3 proteins localized in the nucleus.
Verbatim
e0“Subcellular localization analysis revealed that all seven CsHDZ3s located in the nucleus.”
e1“tea plant genome”
Claim · model-written summary
CsHDZ3-1 to CsHDZ3-4 had trans-activation ability.
Verbatim
e0“CsHDZ3-1 to CsHDZ3-4 have trans-activation ability whereas CsHDZ3-5 to CsHDZ3-7 served as transcriptional inhibitors.”
e1“tea plant genome”
Claim · model-written summary
CsHDZ3-5 to CsHDZ3-7 acted as transcriptional inhibitors.
Verbatim
e0“CsHDZ3-5 to CsHDZ3-7 served as transcriptional inhibitors.”
e1“tea plant genome”
Claim · model-written summary
All seven CsHDZ3 genes responded to simulated natural drought stress.
Not reportedComparatorDirection
Verbatim
e0“all seven CsHDZ3 genes could respond to simulated natural drought stress and polyethylene glycol treatment.”
e1“tea plant genome”
Claim · model-written summary
All seven CsHDZ3 genes responded to polyethylene glycol treatment.
Not reportedComparatorDirection
Verbatim
e0“all seven CsHDZ3 genes could respond to simulated natural drought stress and polyethylene glycol treatment.”
e1“tea plant genome”
Claim · model-written summary
All CsHDZ3 genes could be cleaved by csn-miR166.
Verbatim
e0“all CsHDZ3 genes can be cleaved by csn-miR166.”
e1“tea plant genome”
Claim · model-written summary
Overexpressing csn-miR166 inhibited expression of all seven CsHDZ3 genes.
- Taxon
- teae0
- Treatment
- Overexpression of csn-miR166e0
- Gene
- CsHDZ3 genese0
- Outcome
- expression of seven CsHDZ3 genese0
- Direction
- inhibitede0
Not reportedComparator
Verbatim
e0“Overexpression of csn-miR166 inhibited the expression of seven CsHDZ3 genes and weakened drought tolerance of tea leaves.”
Claim · model-written summary
Overexpressing csn-miR166 weakened drought tolerance of tea leaves.
- Taxon
- teae0
- Organ / stage
- leavese0
- Treatment
- Overexpression of csn-miR166e0
- Outcome
- drought tolerance of tea leavese0
- Direction
- weakenede0
Not reportedComparator
Verbatim
e0“Overexpression of csn-miR166 inhibited the expression of seven CsHDZ3 genes and weakened drought tolerance of tea leaves.”
Claim · model-written summary
Suppressing csn-miR166 promoted expression of all seven CsHDZ3 genes.
- Taxon
- tea plante1
- Treatment
- suppression of csn-miR166e0
- Gene
- CsHDZ3 genese0
- Outcome
- expression of seven CsHDZ3 genese0
- Direction
- promotede0
Not reportedComparator
Verbatim
e0“suppression of csn-miR166 promoted the expression of seven CsHDZ3 genes and enhanced drought tolerance of tea leaves.”
e1“tea plant genome”
Claim · model-written summary
Suppressing csn-miR166 enhanced drought tolerance of tea leaves.
- Taxon
- teae0
- Organ / stage
- leavese0
- Treatment
- suppression of csn-miR166e0
- Outcome
- drought tolerance of tea leavese0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“suppression of csn-miR166 promoted the expression of seven CsHDZ3 genes and enhanced drought tolerance of tea leaves.”
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