Abstract
• UV-B pre-irradiation reduced cold injury in tea plants. • Involvement of CsHY5 in UV-B reduced cold damage in tea plants. • UV-B preirradiation of tea plants enhances cold tolerance by inducing CsHY5 expression and triggering an increase in flavonoid accumulation. Flavonoids derived from the phenylpropane pathway are polyphenolic secondary metabolites in plants, which are involved in various processes of plant growth and abiotic stress. Based on metabolome and transcriptome analysis, we speculated that UV-B irradiation may be a convenient method to improve the cold resistance of tea plants. In our study, tea plants pre-irradiated with 2 μmol·m -2· s -1 UV-B exhibited a cold tolerant phenotype at -4 °C. HY5 plays a pivotal role in mediating the crosstalk between light and temperature signaling pathways in plants. To further explore the internal mechanisms, the role of CsHY5 , acted as UV-B photoreceptors, in cold tolerance was investigated. CsHY5- silenced in tea leaves dramatically impaired UV-B pre-irradiation-enhanced effects on cold tolerance of tea plants, also the contents of flavonoid and the downstream gene expression level of CsMYB12, CsFLS, CsDFR, CsUGT78A14 and CsANR in CsHY5 -silenced tea leaves were significantly decreased. On the contrary, CsHY5 overexpression lines of in Arabidopsis thaliana pre-irradiated with UV-B significantly elevated cold tolerance, and the higher flavonoid content and AtFLS, AtDFR and AtANR expression level was detected compared with WT under cold stress. Therefore, our study indicated UV-B preirradiation of tea plants enhances cold tolerance by inducing CsHY5 expression and triggering an increase in flavonoid accumulation.
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Claims 8
Claim · model-written summary
UV-B pretreatment reduced cold injury in tea plants.
Not reportedComparator
Verbatim
e0“UV-B pre-irradiation reduced cold injury in tea plants”
Claim · model-written summary
Tea plants pre-irradiated with 2 μmol·m -2· s -1 UV-B showed a cold tolerant phenotype at -4 °C.
Verbatim
e0“tea plants pre-irradiated with 2 μmol·m -2· s -1 UV-B exhibited a cold tolerant phenotype at -4 °C”
Claim · model-written summary
CsHY5 silencing impaired UV-B-induced cold tolerance in tea plants.
Not reportedComparator
Verbatim
e0“CsHY5- silenced in tea leaves dramatically impaired UV-B pre-irradiation-enhanced effects on cold tolerance of tea plants”
Claim · model-written summary
CsHY5 silencing significantly decreased flavonoid content in tea leaves.
- Taxon
- tea plantst0
- Treatment
- CsHY5 -silencede0
- Gene
- CsHY5e0
- Outcome
- contents of flavonoide0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“the contents of flavonoid and the downstream gene expression level of CsMYB12, CsFLS, CsDFR, CsUGT78A14 and CsANR in CsHY5 -silenced tea leaves were significantly decreased”
t0“tea plants”
Claim · model-written summary
CsHY5 silencing significantly decreased downstream gene expression in tea leaves.
- Taxon
- tea plantst0
- Treatment
- CsHY5 -silencede0
- Gene
- CsMYB12, CsFLS, CsDFR, CsUGT78A14 and CsANRe0
- Outcome
- downstream gene expression levele0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“the downstream gene expression level of CsMYB12, CsFLS, CsDFR, CsUGT78A14 and CsANR in CsHY5 -silenced tea leaves were significantly decreased”
t0“tea plants”
Claim · model-written summary
UV-B pretreatment of CsHY5 overexpression lines of Arabidopsis increased cold tolerance relative to wild type.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- CsHY5 overexpression linese0
- Treatment
- pre-irradiated with UV-Be0
- Gene
- CsHY5e0
- Outcome
- cold tolerancee0
- Direction
- elevatede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsHY5 overexpression lines of in Arabidopsis thaliana pre-irradiated with UV-B significantly elevated cold tolerance, and the higher flavonoid content and AtFLS, AtDFR and AtANR expression level was detected compared with WT under cold stress”
Claim · model-written summary
UV-B-pretreated CsHY5 overexpression Arabidopsis lines had higher flavonoid content than wild type under cold stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- CsHY5 overexpression linese0
- Gene
- CsHY5e0
- Outcome
- flavonoid contente0
- Direction
- highere0
- Qualifier
- cold stresse0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsHY5 overexpression lines of in Arabidopsis thaliana pre-irradiated with UV-B significantly elevated cold tolerance, and the higher flavonoid content and AtFLS, AtDFR and AtANR expression level was detected compared with WT under cold stress”
Claim · model-written summary
UV-B-pretreated CsHY5 overexpression Arabidopsis lines had higher AtFLS, AtDFR and AtANR expression than wild type under cold stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- CsHY5 overexpression linese0
- Gene
- AtFLS, AtDFR and AtANRe0
- Outcome
- AtFLS, AtDFR and AtANR expression levele0
- Direction
- highere0
- Qualifier
- cold stresse0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsHY5 overexpression lines of in Arabidopsis thaliana pre-irradiated with UV-B significantly elevated cold tolerance, and the higher flavonoid content and AtFLS, AtDFR and AtANR expression level was detected compared with WT under cold stress”
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