Abstract
Seasonal greening is a crucial survival strategy for albino tea cultivars, during which dysfunctional chloroplasts recover and chlorophyll biosynthesis increases in albino leaves. However, the regulatory mechanisms of seasonal greening in albino tea plants remain unclear. Here, we report that CsRVE1, a nuclear-located Myb-like transcription factor, can positively modulate the seasonal greening of albino Camellia sinensis cv. Huangkui leaves by activating the expression of genes involved in light harvesting and chlorophyll biosynthesis. The transcriptional expression of CsRVE1 increased during seasonal greening and was tightly correlated with increases in the expression of genes involved in light harvesting (CsLhcb) and chlorophyll biosynthesis (CsCHLH, CsHEMA1 and CsCAO). In vivo and in vitro molecular analyses showed that CsRVE1 can directly bind to the promoters of CsLhcb, CsCHLH and CsPORA, eventually leading to chlorophyll accumulation in tea leaves. Furthermore, transient suppression of CsRVE1 in tea leaves led to a decrease in target gene expression. In contrast, the overexpression of CsRVE1 in Arabidopsis led to chlorophyll increases and the activation of AtLhcb, AtPORA, AtCHLH, etc. These results identify CsRVE1 as an important promoter of seasonal greening that functions by regulating genes involved in chlorophyll biosynthesis in albino tea plants and shed new light on the regulatory mechanisms of leaf phenotypes in plants.
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Claims 8
Claim · model-written summary
CsRVE1 positively modulated seasonal greening of albino tea leaves through expression of light harvesting and chlorophyll biosynthesis genes.
- Taxon
- Camellia sinensis cv. Huangkui
Camellia sinensise0 - Organ / stage
- leavese0
- Gene
- CsRVE1e0
- Outcome
- seasonal greeninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsRVE1, a nuclear-located Myb-like transcription factor, can positively modulate the seasonal greening of albino Camellia sinensis cv. Huangkui leaves by activating the expression of genes involved in light harvesting and chlorophyll biosynthesis”
Claim · model-written summary
CsRVE1 transcription increased during seasonal greening.
- Taxon
- Camellia sinensis cv. HuangkuiCamellia sinensist1
- Gene
- CsRVE1e0
- Outcome
- transcriptional expression of CsRVE1e0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptional expression of CsRVE1 increased during seasonal greening”
t1“Camellia sinensis cv. Huangkui”
Claim · model-written summary
CsRVE1 transcription correlated with increased expression of CsLhcb, CsCHLH, CsHEMA1 and CsCAO during greening.
- Taxon
- Camellia sinensis cv. HuangkuiCamellia sinensist0
- Gene
- CsRVE1e1
- Outcome
- expression of genes involved in light harvesting (CsLhcb) and chlorophyll biosynthesis (CsCHLH, CsHEMA1 and CsCAO)e0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was tightly correlated with increases in the expression of genes involved in light harvesting (CsLhcb) and chlorophyll biosynthesis (CsCHLH, CsHEMA1 and CsCAO)”
e1“The transcriptional expression of CsRVE1 increased during seasonal greening”
t0“Camellia sinensis cv. Huangkui”
Claim · model-written summary
CsRVE1 directly bound promoters of CsLhcb, CsCHLH and CsPORA.
- Taxon
- Camellia sinensis cv. HuangkuiCamellia sinensist1
- Gene
- CsRVE1e0
- Outcome
- directly bind to the promoters of CsLhcb, CsCHLH and CsPORAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsRVE1 can directly bind to the promoters of CsLhcb, CsCHLH and CsPORA”
t1“Camellia sinensis cv. Huangkui”
Claim · model-written summary
Direct promoter binding by CsRVE1 led to chlorophyll accumulation in tea leaves.
- Taxon
- Camellia sinensis cv. HuangkuiCamellia sinensist1
- Organ / stage
- leavese0
- Gene
- CsRVE1e0
- Outcome
- chlorophyll accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsRVE1 can directly bind to the promoters of CsLhcb, CsCHLH and CsPORA, eventually leading to chlorophyll accumulation in tea leaves”
t1“Camellia sinensis cv. Huangkui”
Claim · model-written summary
Transient suppression of CsRVE1 decreased target gene expression in tea leaves.
- Taxon
- Camellia sinensis cv. HuangkuiCamellia sinensist1
- Treatment
- transient suppression of CsRVE1e0
- Gene
- CsRVE1e0
- Outcome
- target gene expressione0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient suppression of CsRVE1 in tea leaves led to a decrease in target gene expression”
t1“Camellia sinensis cv. Huangkui”
Claim · model-written summary
Overexpression of CsRVE1 increased chlorophyll in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of CsRVE1e0
- Gene
- CsRVE1e0
- Outcome
- chlorophylle0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the overexpression of CsRVE1 in Arabidopsis led to chlorophyll increases and the activation of AtLhcb, AtPORA, AtCHLH”
t1“Camellia sinensis cv. Huangkui”
Claim · model-written summary
Overexpression of CsRVE1 activated AtLhcb, AtPORA and AtCHLH in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of CsRVE1e0
- Gene
- CsRVE1e0
- Outcome
- activation of AtLhcb, AtPORA, AtCHLHe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the overexpression of CsRVE1 in Arabidopsis led to chlorophyll increases and the activation of AtLhcb, AtPORA, AtCHLH”
t1“Camellia sinensis cv. Huangkui”
The same questions in other papers
loss of function · molecular: expression · Camellia sinensis (black tea)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.