Abstract
Cold and drought stresses severely limit crop production and can occur simultaneously. Although some transcription factors and hormones have been characterized in plants subjected each stress, the role of metabolites, especially volatiles, in response to cold and drought stress exposure is rarely studied due to lack of suitable models. Here, we established a model for studying the role of volatiles in tea (Camellia sinensis) plants experiencing cold and drought stresses simultaneously. Using this model, we showed that volatiles induced by cold stress promote drought tolerance in tea plants by mediating reactive oxygen species and stomatal conductance. Needle trap microextraction combined with GC-MS identified the volatiles involved in the crosstalk and showed that cold-induced (Z)-3-hexenol improved the drought tolerance of tea plants. In addition, silencing C. sinensis alcohol dehydrogenase 2 (CsADH2) led to reduced (Z)-3-hexenol production and significantly reduced drought tolerance in response to simultaneous cold and drought stress. Transcriptome and metabolite analyses, together with plant hormone comparison and abscisic acid (ABA) biosynthesis pathway inhibition experiments, further confirmed the roles of ABA in (Z)-3-hexenol-induced drought tolerance of tea plants. (Z)-3-Hexenol application and gene silencing results supported the hypothesis that (Z)-3-hexenol plays a role in the integration of cold and drought tolerance by stimulating the dual-function glucosyltransferase UGT85A53, thereby altering ABA homeostasis in tea plants. Overall, we present a model for studying the roles of metabolites in plants under multiple stresses and reveal the roles of volatiles in integrating cold and drought stresses in plants.
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Claims 6
Claim · model-written summary
Cold-induced volatiles promoted tea drought tolerance through reactive oxygen species and stomatal conductance.
Verbatim
e0“volatiles induced by cold stress promote drought tolerance in tea plants by mediating reactive oxygen species and stomatal conductance”
Claim · model-written summary
Cold-induced (Z)-3-hexenol improved tea drought tolerance.
Not reportedComparator
Verbatim
e0“cold-induced (Z)-3-hexenol improved the drought tolerance of tea plants”
Claim · model-written summary
Silencing CsADH2 reduced (Z)-3-hexenol production in tea.
- Taxon
- teae1
- Treatment
- silencing C. sinensis alcohol dehydrogenase 2 (CsADH2)e0
- Gene
- C. sinensis alcohol dehydrogenase 2 (CsADH2)e0
- Outcome
- (Z)-3-hexenol productione0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“silencing C. sinensis alcohol dehydrogenase 2 (CsADH2) led to reduced (Z)-3-hexenol production”
e1“tea (Camellia sinensis) plants”
Claim · model-written summary
Silencing CsADH2 significantly reduced tea drought tolerance under simultaneous cold and drought.
- Taxon
- teae1
- Treatment
- silencing C. sinensis alcohol dehydrogenase 2 (CsADH2)e0
- Gene
- C. sinensis alcohol dehydrogenase 2 (CsADH2)e0
- Outcome
- drought tolerancee0
- Direction
- reducede0
- Qualifier
- simultaneous cold and drought stresse0
Not reportedComparator
Verbatim
e0“silencing C. sinensis alcohol dehydrogenase 2 (CsADH2) led to reduced (Z)-3-hexenol production and significantly reduced drought tolerance in response to simultaneous cold and drought stress”
e1“tea (Camellia sinensis) plants”
Claim · model-written summary
Analyses and inhibition experiments confirmed an ABA role in (Z)-3-hexenol-induced drought tolerance.
Verbatim
e0“confirmed the roles of ABA in (Z)-3-hexenol-induced drought tolerance of tea plants”
Claim · model-written summary
The authors infer (Z)-3-hexenol integrates cold and drought tolerance by stimulating UGT85A53 and altering ABA homeostasis.
Verbatim
e0“(Z)-3-hexenol plays a role in the integration of cold and drought tolerance by stimulating the dual-function glucosyltransferase UGT85A53, thereby altering ABA homeostasis in tea plants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.