Abstract
Agricultural crops are susceptible to abiotic and biotic factors. Among biotic factors, plant pathogens attacks are prominent and significantly reduce the crop productivity and production. Long term application of synthetic pesticides in agriculture not only causes resistance development in pathogens but also deteriorates the soil quality and pollutes the ecosystem. In the current study, an investigation has been made with an objective to isolate an endophytic bacterium from Withania somnifera seed and evaluate its potential biocontrol activity against soil-borne pathogens like Fusarium oxysporum var. ciceri and Rhizoctonia solani. The molecular approach of bacterium identification using 16S rRNA sequencing methods includes kit based DNA extraction and PCR amplification and the isolated endophytic bacterium was identified as Pseudomonas stutzeri. In dual culture assay, the endophytic bacterium P. stutzeri PSIISS-1 effectively suppressed growth of pathogenic fungi F. oxysporum var. ciceri (61.0%) and R. solani (53.3%), respectively. In vivo studies were conducted with initial microbial inoculum size of 108cell/ml and the studies revealed that the bacterium P. stutzeri PSIISS-1 co-inoculated with R. solani had significantly inhibited the sheath blight disease with the efficacy of 76% in susceptible paddy MTU-7029. Simultaneously, co-inoculation of P. stutzeri PSIISS-1 along with the pathogen F. oxysporum var. ciceri in chickpea JG-14 variety resulted in reduction of wilt symptom up to 80.45%. Bacterial inhibitions against the pathogens were corroborated with chitinase, protease and lipase enzymatic activities. Based on the results obtained from the study, P. stutzeri showed biocontrol activity against plant pathogens which can be used as potential bio-pesticides.
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Claims 7
Claim · model-written summary
The seed endophyte was identified as Pseudomonas stutzeri.
- Taxon
- Withania somnifera
Withania somniferae1 - Interactor
- Pseudomonas stutzerie0symbiont
- Outcome
- identified as Pseudomonas stutzerie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the isolated endophytic bacterium was identified as Pseudomonas stutzeri”
e1“endophytic bacterium from Withania somnifera seed”
Claim · model-written summary
P. stutzeri PSIISS-1 suppressed F. oxysporum var. ciceri growth by 61.0% in dual culture.
- Taxon
- Withania somniferaWithania somniferat0
- Treatment
- P. stutzeri PSIISS-1e0
- Interactor
- F. oxysporum var. ciceri
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Second interactor
- P. stutzeri PSIISS-1e0biocontrol_agent
- Outcome
- growth of pathogenic fungi F. oxysporum var. cicerie0
- Direction
- suppressede0
- Value
- 61.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“the endophytic bacterium P. stutzeri PSIISS-1 effectively suppressed growth of pathogenic fungi F. oxysporum var. ciceri (61.0%)”
t0“Withania somnifera (Ashwagandha)”
Claim · model-written summary
P. stutzeri PSIISS-1 suppressed R. solani growth by 53.3% in dual culture.
- Taxon
- Withania somniferaWithania somniferat0
- Treatment
- P. stutzeri PSIISS-1e0
- Interactor
- R. solani
Rhizoctonia solanie0pathogen - Second interactor
- P. stutzeri PSIISS-1e0biocontrol_agent
- Outcome
- growth of pathogenic fungie0
- Direction
- suppressede0
- Value
- 53.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. stutzeri PSIISS-1 effectively suppressed growth of pathogenic fungi F. oxysporum var. ciceri (61.0%) and R. solani (53.3%)”
t0“Withania somnifera (Ashwagandha)”
Claim · model-written summary
Co-inoculation with P. stutzeri PSIISS-1 significantly inhibited sheath blight in susceptible paddy MTU-7029 with 76% efficacy.
- Taxon
- paddye0
- Material
- MTU-7029e0
- Treatment
- P. stutzeri PSIISS-1 co-inoculated with R. solanie0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Second interactor
- P. stutzeri PSIISS-1e0biocontrol_agent
- Outcome
- sheath blight diseasee0
- Direction
- inhibitede0
- Value
- 76%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. stutzeri PSIISS-1 co-inoculated with R. solani had significantly inhibited the sheath blight disease with the efficacy of 76% in susceptible paddy MTU-7029”
Claim · model-written summary
Co-inoculation with P. stutzeri PSIISS-1 reduced wilt symptoms up to 80.45% in chickpea JG-14.
- Taxon
- chickpea
Cicer arietinume0 - Material
- JG-14e0
- Treatment
- co-inoculation of P. stutzeri PSIISS-1 along with the pathogen F. oxysporum var. cicerie0
- Interactor
- F. oxysporum var. ciceriFusarium oxysporume0pathogen
- Second interactor
- P. stutzeri PSIISS-1e0biocontrol_agent
- Outcome
- wilt symptome0
- Direction
- reductione0
- Value
- 80.45%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-inoculation of P. stutzeri PSIISS-1 along with the pathogen F. oxysporum var. ciceri in chickpea JG-14 variety resulted in reduction of wilt symptom up to 80.45%”
Claim · model-written summary
Chitinase, protease and lipase activities corroborated bacterial inhibition of the pathogens.
- Taxon
- Withania somniferaWithania somniferae1
- Gene
- chitinase, protease and lipasee0
- Outcome
- Bacterial inhibitions against the pathogense0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacterial inhibitions against the pathogens were corroborated with chitinase, protease and lipase enzymatic activities.”
e1“endophytic bacterium from Withania somnifera seed”
Claim · model-written summary
P. stutzeri was proposed as a potential bio-pesticide against plant pathogens.
- Taxon
- Withania somniferaWithania somniferae1
- Interactor
- P. stutzerie0biocontrol_agent
- Outcome
- potential bio-pesticidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. stutzeri showed biocontrol activity against plant pathogens which can be used as potential bio-pesticides”
e1“endophytic bacterium from Withania somnifera seed”
The same questions in other papers
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105 papers in the same direction as this paper · 51 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
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See all 158 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.