Abstract
Potato invertase inhibitor (StInvInh2) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns of StInvInh2 have been thoroughly characterized in different potato genotypes, but the related CIS ability has not been characterized. The understanding of the regulatory mechanisms that control StInvInh2 transcription is unclear. In this study, we identified an ERF-VII transcription factor, StRAP2.3, that directly regulates StInvInh2 to positively modulate CIS resistance. Acting as a nuclear-localized transcriptional activator, StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of StInvInh2, enabling promoter activity. Overexpression of StRAP2.3 in CIS-sensitive potato tubers induced StInvInh2 mRNA abundance and increased CIS resistance. In contrast, silencing StRAP2.3 in CIS-resistant potato tubers repressed the expression of StInvInh2 and decreased CIS resistance. We conclude that cold-responsive StInvInh2 is due to the binding of StRAP2.3 to the ACCGAC cis-element in the promoter region of StInvInh2. Overall, these findings indicate that StRAP2.3 directly regulates StInvInh2 to positively modulate CIS resistance, which may provide a strategy to improve the processing quality of potatoes.
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Claims 6
Claim · model-written summary
StRAP2.3 was identified as a regulator of StInvInh2 and CIS resistance.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- StRAP2.3e0
- Outcome
- directly regulates StInvInh2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified an ERF-VII transcription factor, StRAP2.3, that directly regulates StInvInh2 to positively modulate CIS resistance”
t0“in potato”
Claim · model-written summary
StRAP2.3 binds the ACCGAC promoter element and enables StInvInh2 promoter activity.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StRAP2.3e0
- Outcome
- promoter activitye0
- Qualifier
- directly binds the ACCGAC cis-element in the promoter region of StInvInh2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StRAP2.3 directly binds the ACCGAC cis-element in the promoter region of StInvInh2, enabling promoter activity”
t0“in potato”
Claim · model-written summary
Overexpressing StRAP2.3 increased StInvInh2 mRNA abundance in CIS-sensitive potato tubers.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tuberse0
- Treatment
- Overexpression of StRAP2.3e0
- Outcome
- StInvInh2 mRNA abundancee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of StRAP2.3 in CIS-sensitive potato tubers induced StInvInh2 mRNA abundance and increased CIS resistance”
Claim · model-written summary
Overexpressing StRAP2.3 increased CIS resistance.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tuberse0
- Treatment
- Overexpression of StRAP2.3e0
- Outcome
- CIS resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of StRAP2.3 in CIS-sensitive potato tubers induced StInvInh2 mRNA abundance and increased CIS resistance”
Claim · model-written summary
Silencing StRAP2.3 repressed StInvInh2 expression in resistant tubers.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tuberse0
- Treatment
- silencing StRAP2.3e0
- Outcome
- expression of StInvInh2e0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing StRAP2.3 in CIS-resistant potato tubers repressed the expression of StInvInh2 and decreased CIS resistance”
Claim · model-written summary
Silencing StRAP2.3 decreased CIS resistance.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tuberse0
- Treatment
- silencing StRAP2.3e0
- Outcome
- CIS resistancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing StRAP2.3 in CIS-resistant potato tubers repressed the expression of StInvInh2 and decreased CIS resistance”
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