Abstract
Cold-induced sweetening (CIS) is a serious post-harvest problem for potato tubers, which need to be stored cold to prevent sprouting and pathogenesis in order to maintain supply throughout the year. During storage at cold temperatures (below 10 °C), many cultivars accumulate free reducing sugars derived from a breakdown of starch to sucrose that is ultimately cleaved by acid invertase to produce glucose and fructose. When affected tubers are processed by frying or roasting, these reducing sugars react with free asparagine by the Maillard reaction, resulting in unacceptably dark-coloured and bitter-tasting product and generating the probable carcinogen acrylamide as a by-product. We have previously identified a vacuolar invertase inhibitor (INH2) whose expression correlates both with low acid invertase activity and with resistance to CIS. Here we show that, during cold storage, overexpression of the INH2 vacuolar invertase inhibitor gene in CIS-susceptible potato tubers reduced acid invertase activity, the accumulation of reducing sugars and the generation of acrylamide in subsequent fry tests. Conversely, suppression of vacuolar invertase inhibitor expression in a CIS-resistant line increased susceptibility to CIS. The results show that post-translational regulation of acid invertase by the vacuolar invertase inhibitor is an important component of resistance to CIS.
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Claims 5
Claim · model-written summary
INH2 overexpression reduced acid invertase activity.
- Taxon
- potato tuberst0
- Treatment
- overexpression of the INH2 vacuolar invertase inhibitor genee0
- Gene
- INH2 vacuolar invertase inhibitor genee0
- Outcome
- acid invertase activitye0
- Direction
- reducede0
Not reportedComparatorQualifier
Verbatim
t0“potato tubers”
e0“overexpression of the INH2 vacuolar invertase inhibitor gene in CIS-susceptible potato tubers reduced acid invertase activity, the accumulation of reducing sugars and the generation of acrylamide in subsequent fry tests.”
Claim · model-written summary
INH2 overexpression reduced accumulation of reducing sugars.
- Taxon
- potato tuberst0
- Treatment
- overexpression of the INH2 vacuolar invertase inhibitor genee0
- Gene
- INH2 vacuolar invertase inhibitor genee0
- Outcome
- accumulation of reducing sugarse0
- Direction
- reducede0
Not reportedComparatorQualifier
Verbatim
t0“potato tubers”
e0“overexpression of the INH2 vacuolar invertase inhibitor gene in CIS-susceptible potato tubers reduced acid invertase activity, the accumulation of reducing sugars and the generation of acrylamide in subsequent fry tests.”
Claim · model-written summary
INH2 overexpression reduced generation of acrylamide.
- Taxon
- potato tuberst0
- Treatment
- overexpression of the INH2 vacuolar invertase inhibitor genee0
- Gene
- INH2 vacuolar invertase inhibitor genee0
- Outcome
- generation of acrylamidee0
- Direction
- reducede0
Not reportedComparatorQualifier
Verbatim
t0“potato tubers”
e0“overexpression of the INH2 vacuolar invertase inhibitor gene in CIS-susceptible potato tubers reduced acid invertase activity, the accumulation of reducing sugars and the generation of acrylamide in subsequent fry tests.”
Claim · model-written summary
Suppressing vacuolar invertase inhibitor expression increased CIS susceptibility in a resistant line.
- Taxon
- potato tuberst0
- Material
- CIS-resistant linee0
- Treatment
- suppression of vacuolar invertase inhibitor expressione0
- Outcome
- susceptibility to CISe0
- Direction
- increasede0
Not reportedComparator
Verbatim
t0“potato tubers”
e0“suppression of vacuolar invertase inhibitor expression in a CIS-resistant line increased susceptibility to CIS.”
Claim · model-written summary
The authors infer that vacuolar invertase inhibitor regulation of acid invertase contributes to CIS resistance.
Verbatim
t0“potato tubers”
e0“post-translational regulation of acid invertase by the vacuolar invertase inhibitor is an important component of resistance to CIS.”
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loss of function · disease · Solanum tuberosum (potato)Positive and negative results
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.