Abstract
The accumulation of reducing sugars in cold-stored tubers, known as cold-induced sweetening (CIS), negatively affects potato processing quality. The starch to sugar interconversion pathways that are altered in cold-stored CIS tubers have been elucidated, but the mechanism that regulates them remains largely unknown. This study identified a CBF/DREB transcription factor (StTINY3) that enhances CIS resistance by both activating starch biosynthesis and repressing the hydrolysis of sucrose to reducing sugars in detached cold-stored tubers. Silencing StTINY3 in a CIS-resistant genotype decreased CIS resistance, while overexpressing StTINY3 in a CIS-sensitive genotype increased CIS resistance, and altering StTINY3 expression was associated with expression changes in starch resynthesis-related genes. We showed first that overexpressing StTINY3 inhibited sucrose hydrolysis by enhancing expression of the invertase inhibitor gene StInvInh2, and second that StTINY3 promoted starch resynthesis by up-regulating a large subunit of the ADP-glucose pyrophosphorylase gene StAGPaseL3, and the glucose-6-phosphate transporter gene StG6PT2. Using electrophoretic mobility shift assays, we revealed that StTINY3 is a nuclear-localized transcriptional activator that directly binds to the dehydration-responsive element/CRT cis-element in the promoters of StInvInh2 and StAGPaseL3. Taken together, these findings established that StTINY3 influences CIS resistance in cold-stored tubers by coordinately modulating the starch to sugar interconversion pathways and is a good target for improving potato processing quality.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
StTINY3 enhanced cold-induced sweetening resistance by activating starch biosynthesis.
- Taxon
- potato
Solanum tuberosumt0 - Organ / stage
- tuberse0
- Gene
- StTINY3e0
- Outcome
- CIS resistancee0
- Qualifier
- activating starch biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified a CBF/DREB transcription factor (StTINY3) that enhances CIS resistance by both activating starch biosynthesis and repressing the hydrolysis of sucrose to reducing sugars in detached cold-stored tubers”
t0“potato”
Claim · model-written summary
StTINY3 enhanced cold-induced sweetening resistance by repressing sucrose hydrolysis.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Gene
- StTINY3e0
- Outcome
- CIS resistancee0
- Qualifier
- repressing the hydrolysis of sucrose to reducing sugarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified a CBF/DREB transcription factor (StTINY3) that enhances CIS resistance by both activating starch biosynthesis and repressing the hydrolysis of sucrose to reducing sugars in detached cold-stored tubers”
t0“potato”
Claim · model-written summary
Silencing StTINY3 reduced CIS resistance in a resistant genotype.
- Taxon
- potatoSolanum tuberosumt0
- Material
- CIS-resistant genotypee0
- Treatment
- Silencing StTINY3e0
- Gene
- StTINY3e0
- Outcome
- CIS resistancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing StTINY3 in a CIS-resistant genotype decreased CIS resistance”
t0“potato”
Claim · model-written summary
Overexpressing StTINY3 increased CIS resistance in a sensitive genotype.
- Taxon
- potatoSolanum tuberosumt0
- Material
- CIS-sensitive genotypee0
- Treatment
- overexpressing StTINY3e0
- Gene
- StTINY3e0
- Outcome
- CIS resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing StTINY3 in a CIS-sensitive genotype increased CIS resistance”
t0“potato”
Claim · model-written summary
Altered StTINY3 expression was associated with starch resynthesis gene expression changes.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StTINY3e0
- Outcome
- expression changes in starch resynthesis-related genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altering StTINY3 expression was associated with expression changes in starch resynthesis-related genes”
t0“potato”
Claim · model-written summary
StTINY3 overexpression inhibited sucrose hydrolysis through enhanced StInvInh2 expression.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- overexpressing StTINY3e0
- Gene
- StInvInh2e0
- Outcome
- sucrose hydrolysise0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing StTINY3 inhibited sucrose hydrolysis by enhancing expression of the invertase inhibitor gene StInvInh2”
t0“potato”
Claim · model-written summary
StTINY3 promoted starch resynthesis through upregulation of StAGPaseL3.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StAGPaseL3e0
- Outcome
- starch resynthesise0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StTINY3 promoted starch resynthesis by up-regulating a large subunit of the ADP-glucose pyrophosphorylase gene StAGPaseL3, and the glucose-6-phosphate transporter gene StG6PT2”
t0“potato”
Claim · model-written summary
StTINY3 promoted starch resynthesis through upregulation of StG6PT2.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StG6PT2e0
- Outcome
- starch resynthesise0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StTINY3 promoted starch resynthesis by up-regulating a large subunit of the ADP-glucose pyrophosphorylase gene StAGPaseL3, and the glucose-6-phosphate transporter gene StG6PT2”
t0“potato”
Claim · model-written summary
StTINY3 localized to the nucleus and acted as a transcriptional activator.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StTINY3e0
- Outcome
- nuclear-localized transcriptional activatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StTINY3 is a nuclear-localized transcriptional activator”
t0“potato”
Claim · model-written summary
StTINY3 directly bound a cis-element in the StInvInh2 promoter.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StInvInh2e0
- Outcome
- directly binds to the dehydration-responsive element/CRT cis-elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StTINY3 is a nuclear-localized transcriptional activator that directly binds to the dehydration-responsive element/CRT cis-element in the promoters of StInvInh2 and StAGPaseL3”
t0“potato”
Claim · model-written summary
StTINY3 directly bound a cis-element in the StAGPaseL3 promoter.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StAGPaseL3e0
- Outcome
- directly binds to the dehydration-responsive element/CRT cis-elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StTINY3 is a nuclear-localized transcriptional activator that directly binds to the dehydration-responsive element/CRT cis-element in the promoters of StInvInh2 and StAGPaseL3”
t0“potato”
The same questions in other papers
loss of function · disease · Solanum tuberosum (potato)Positive and negative results
11 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- positive
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.