Abstract
Abstract Root–shoot communication has a critical role in plant adaptation to environmental stress. Grafting is widely applied to enhance the abiotic stress tolerance of many horticultural crop species; however, the signal transduction mechanism involved in this tolerance remains unknown. Here, we show that pumpkin- or figleaf gourd rootstock-enhanced cold tolerance of watermelon shoots is accompanied by increases in the accumulation of melatonin, methyl jasmonate (MeJA), and hydrogen peroxide (H 2 O 2 ). Increased melatonin levels in leaves were associated with both increased melatonin in rootstocks and MeJA-induced melatonin biosynthesis in leaves of plants under cold stress. Exogenous melatonin increased the accumulation of MeJA and H 2 O 2 and enhanced cold tolerance, while inhibition of melatonin accumulation attenuated rootstock-induced MeJA and H 2 O 2 accumulation and cold tolerance. MeJA application induced H 2 O 2 accumulation and cold tolerance, but inhibition of JA biosynthesis abolished rootstock- or melatonin-induced H 2 O 2 accumulation and cold tolerance. Additionally, inhibition of H 2 O 2 production attenuated MeJA-induced tolerance to cold stress. Taken together, our results suggest that melatonin is involved in grafting-induced cold tolerance by inducing the accumulation of MeJA and H 2 O 2 . MeJA subsequently increases melatonin accumulation, forming a self-amplifying feedback loop that leads to increased H 2 O 2 accumulation and cold tolerance. This study reveals a novel regulatory mechanism of rootstock-induced cold tolerance.
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Claims 14
Claim · model-written summary
Pumpkin or figleaf gourd rootstock enhanced the cold tolerance of watermelon shoots.
- Taxon
- watermelon
Citrullus lanatuse1 - Treatment
- pumpkin- or figleaf gourd rootstocke1
- Outcome
- cold tolerancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pumpkin- or figleaf gourd rootstock-enhanced cold tolerance of watermelon shoots is accompanied by increases in the accumulation of melatonin, methyl jasmonate (MeJA), and hydrogen peroxide (H 2 O 2 )”
Claim · model-written summary
Pumpkin or figleaf gourd rootstock increased accumulation of melatonin, MeJA and H2O2 in watermelon shoots.
- Taxon
- watermelonCitrullus lanatuse1
- Treatment
- pumpkin- or figleaf gourd rootstocke1
- Outcome
- accumulation of melatonin, methyl jasmonate (MeJA), and hydrogen peroxide (H 2 O 2 )e1
- Direction
- increasese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“pumpkin- or figleaf gourd rootstock-enhanced cold tolerance of watermelon shoots is accompanied by increases in the accumulation of melatonin, methyl jasmonate (MeJA), and hydrogen peroxide (H 2 O 2 )”
Claim · model-written summary
Increased melatonin levels in leaves were associated with increased melatonin in rootstocks and with MeJA-induced melatonin biosynthesis in leaves of plants under cold stress.
- Taxon
- watermelonCitrullus lanatust0
- Organ / stage
- leavese1
- Treatment
- cold stresse1
- Compound
- MeJAe1
- Outcome
- melatonin levels in leavese1
- Direction
- Increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“Increased melatonin levels in leaves were associated with both increased melatonin in rootstocks and MeJA-induced melatonin biosynthesis in leaves of plants under cold stress”
Claim · model-written summary
Exogenous melatonin increased the accumulation of MeJA and H2O2 in watermelon under cold stress.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- Exogenous melatonine1
- Outcome
- accumulation of MeJA and H 2 O 2e1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“Exogenous melatonin increased the accumulation of MeJA and H 2 O 2 and enhanced cold tolerance”
Claim · model-written summary
Exogenous melatonin enhanced cold tolerance of watermelon.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- Exogenous melatonine1
- Outcome
- cold tolerancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“Exogenous melatonin increased the accumulation of MeJA and H 2 O 2 and enhanced cold tolerance”
Claim · model-written summary
Inhibition of melatonin accumulation attenuated rootstock-induced MeJA and H2O2 accumulation.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inhibition of melatonin accumulatione1
- Outcome
- rootstock-induced MeJA and H 2 O 2 accumulatione1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“inhibition of melatonin accumulation attenuated rootstock-induced MeJA and H 2 O 2 accumulation and cold tolerance”
Claim · model-written summary
Inhibition of melatonin accumulation attenuated rootstock-induced cold tolerance.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inhibition of melatonin accumulatione1
- Outcome
- cold tolerancee1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“inhibition of melatonin accumulation attenuated rootstock-induced MeJA and H 2 O 2 accumulation and cold tolerance”
Claim · model-written summary
MeJA application induced H2O2 accumulation in watermelon.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MeJA applicatione1
- Outcome
- H 2 O 2 accumulatione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“MeJA application induced H 2 O 2 accumulation and cold tolerance”
Claim · model-written summary
MeJA application induced cold tolerance in watermelon.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MeJA applicatione1
- Outcome
- cold tolerancee1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“MeJA application induced H 2 O 2 accumulation and cold tolerance”
Claim · model-written summary
Inhibition of JA biosynthesis abolished rootstock- or melatonin-induced H2O2 accumulation.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inhibition of JA biosynthesise1
- Outcome
- H 2 O 2 accumulatione1
- Direction
- abolishede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“inhibition of JA biosynthesis abolished rootstock- or melatonin-induced H 2 O 2 accumulation and cold tolerance”
Claim · model-written summary
Inhibition of JA biosynthesis abolished rootstock- or melatonin-induced cold tolerance.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inhibition of JA biosynthesise1
- Outcome
- cold tolerancee1
- Direction
- abolishede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“inhibition of JA biosynthesis abolished rootstock- or melatonin-induced H 2 O 2 accumulation and cold tolerance”
Claim · model-written summary
Inhibition of H2O2 production attenuated MeJA-induced tolerance to cold stress.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- inhibition of H 2 O 2 productione1
- Outcome
- MeJA-induced tolerance to cold stresse1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“inhibition of H 2 O 2 production attenuated MeJA-induced tolerance to cold stress”
Claim · model-written summary
The authors conclude that melatonin is involved in grafting-induced cold tolerance by inducing the accumulation of MeJA and H2O2.
- Taxon
- watermelonCitrullus lanatust0
- Compound
- melatonine1
- Outcome
- grafting-induced cold tolerancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“melatonin is involved in grafting-induced cold tolerance by inducing the accumulation of MeJA and H 2 O 2”
Claim · model-written summary
The authors conclude that MeJA subsequently increases melatonin accumulation, forming a self-amplifying feedback loop leading to increased H2O2 accumulation and cold tolerance.
- Taxon
- watermelonCitrullus lanatust0
- Compound
- MeJAe1
- Outcome
- increased H 2 O 2 accumulation and cold tolerancee1
- Qualifier
- self-amplifying feedback loope1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“watermelon”
e1“MeJA subsequently increases melatonin accumulation, forming a self-amplifying feedback loop that leads to increased H 2 O 2 accumulation and cold tolerance”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.