Abstract
In vitro and in VIVO studies and proline analysis were carried out during 2002- 2003 seasons on two Egyptian cultivars of date palm (Zaghloul and Samani). The aims of these studies were tested the tolerant to in vitro and in vivo saline conditions in the two cultivars. Also.study the relationship between the proline content and the tolerant to saline conditions in these cunivars. Data revealed that all treatments of mercuric chloride gave the lowest contamination percentages (0.0% in both cultivars) whilst the treatment of Clorox al 10% for 15 min, gave the highest contamination percentages (70% in Zaghloul and 80% in Samani). The lowest survival percentages in both cultivars were obtained by the treatment of mercuric chloride at 0.1% for 10 min. The treatment of Clorox allO% for 15 min. gave the highest survival percentage in borh cullivars (100% in Zaghloul and Samani). while the treatment of mercuric chloride at 0.1% for 5 min. gave the highest survival disinfected explant percentage (60% in Zaghloul and 70% in Samani). Zaghloul cultivar was superior to Samani cultivar in averages of weight and diameter of callus; number and lenglh of the roots from in vitro shoots and the growth parameters in the acclimatization stage. On the other hend.Samani cultivar was superior to Zaghloul cultivar in averages of number and length ot in vitro shoots and growth parameters of in vitro embryos. The treatment of 7000 p.p.m. salts gave the lowest weight and diameter of the callus, the lowest number and length of in vitro shoots ; the lowest number and length of in vitro roots from in vitro shoots and the lowest growth of in vitro embryos compared with untreat. In vivo study showed that irrigation with solutions of 8000,9000 and 10000 ppm. salts gave the worest results (growth parameters) in the acclimatization stage for the two studied cultivars compared with the irrigalion with waler (control or untreat). There were reversely proportional between concentration of the salts and the growth parameters of the two studied cuftivars in either in vitro or in vivo conditions. Zagh/cul cultivar had more proline content than Saman! cultivar and exhibited more tolerant to either in vitro or in vivo saline conditions which reflected positive relationship between (he proline contents and the tolerant to saline conditions in the two cullivars.
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Claims 20
Claim · model-written summary
Mercuric chloride gave zero explant contamination in both cultivars.
- Taxon
- DATE PALM
Phoenix dactyliferat0 - Treatment
- mercuric chloridee0
- Outcome
- contamination percentagese0
- Direction
- loweste0
- Value
- 0.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all treatments of mercuric chloride gave the lowest contamination percentages (0.0% in both cultivars)”
t0“DATE PALM”
Claim · model-written summary
Clorox at 10% for 15 minutes yielded 70% contamination in Zaghloul.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Zaghloule0
- Treatment
- Clorox al 10% for 15 mine0
- Outcome
- contamination percentagese0
- Direction
- higheste0
- Value
- 70%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clorox al 10% for 15 min, gave the highest contamination percentages (70% in Zaghloul and 80% in Samani)”
t0“DATE PALM”
Claim · model-written summary
Clorox at 10% for 15 minutes yielded 80% contamination in Samani.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Samanie0
- Treatment
- Clorox al 10% for 15 mine0
- Outcome
- contamination percentagese0
- Direction
- higheste0
- Value
- 80%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clorox al 10% for 15 min, gave the highest contamination percentages (70% in Zaghloul and 80% in Samani)”
t0“DATE PALM”
Claim · model-written summary
Mercuric chloride at 0.1% for 10 minutes gave the lowest survival in both cultivars.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- mercuric chloride at 0.1% for 10 min.e0
- Outcome
- survival percentagese0
- Direction
- loweste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lowest survival percentages in both cultivars were obtained by the treatment of mercuric chloride at 0.1% for 10 min.”
t0“DATE PALM”
Claim · model-written summary
Clorox treatment gave 100% survival in Zaghloul.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Zaghloule0
- Treatment
- Clorox allO% for 15 min.e0
- Outcome
- survival percentagee0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clorox allO% for 15 min. gave the highest survival percentage in borh cullivars (100% in Zaghloul and Samani)”
t0“DATE PALM”
Claim · model-written summary
Clorox treatment gave 100% survival in Samani.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Samanie0
- Treatment
- Clorox allO% for 15 min.e0
- Outcome
- survival percentagee0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clorox allO% for 15 min. gave the highest survival percentage in borh cullivars (100% in Zaghloul and Samani)”
t0“DATE PALM”
Claim · model-written summary
Mercuric chloride for 5 minutes gave 60% surviving disinfected explants in Zaghloul.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Zaghloule0
- Treatment
- mercuric chloride at 0.1% for 5 min.e0
- Outcome
- survival disinfected explant percentagee0
- Direction
- higheste0
- Value
- 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mercuric chloride at 0.1% for 5 min. gave the highest survival disinfected explant percentage (60% in Zaghloul and 70% in Samani)”
t0“DATE PALM”
Claim · model-written summary
Mercuric chloride for 5 minutes gave 70% surviving disinfected explants in Samani.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Samanie0
- Treatment
- mercuric chloride at 0.1% for 5 min.e0
- Outcome
- survival disinfected explant percentagee0
- Direction
- higheste0
- Value
- 70%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mercuric chloride at 0.1% for 5 min. gave the highest survival disinfected explant percentage (60% in Zaghloul and 70% in Samani)”
t0“DATE PALM”
Claim · model-written summary
Zaghloul exceeded Samani for callus weight and diameter.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Zaghloule0
- Outcome
- weight and diameter of calluse0
- Direction
- superiore0
- Comparator
- Samanie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zaghloul cultivar was superior to Samani cultivar in averages of weight and diameter of callus”
t0“DATE PALM”
Claim · model-written summary
Zaghloul exceeded Samani for root number and length from in vitro shoots.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Zaghloule0
- Outcome
- number and lenglh of the rootse0
- Direction
- superiore0
- Comparator
- Samanie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zaghloul cultivar was superior to Samani cultivar in averages of weight and diameter of callus; number and lenglh of the roots from in vitro shoots and the growth parameters in the acclimatization stage”
t0“DATE PALM”
Claim · model-written summary
Zaghloul exceeded Samani for acclimatization growth parameters.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Zaghloule0
- Outcome
- growth parameters in the acclimatization stagee0
- Direction
- superiore0
- Comparator
- Samanie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zaghloul cultivar was superior to Samani cultivar in averages of weight and diameter of callus; number and lenglh of the roots from in vitro shoots and the growth parameters in the acclimatization stage”
t0“DATE PALM”
Claim · model-written summary
Samani exceeded Zaghloul for in vitro shoot number and length.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Samanie0
- Outcome
- number and length ot in vitro shootse0
- Direction
- superiore0
- Comparator
- Zaghloule0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Samani cultivar was superior to Zaghloul cultivar in averages of number and length ot in vitro shoots and growth parameters of in vitro embryos”
t0“DATE PALM”
Claim · model-written summary
Samani exceeded Zaghloul for in vitro embryo growth parameters.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Material
- Samanie0
- Outcome
- growth parameters of in vitro embryose0
- Direction
- superiore0
- Comparator
- Zaghloule0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Samani cultivar was superior to Zaghloul cultivar in averages of number and length ot in vitro shoots and growth parameters of in vitro embryos”
t0“DATE PALM”
Claim · model-written summary
At 7000 p.p.m. salts, callus weight and diameter were lowest versus untreated.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- 7000 p.p.m. saltse0
- Outcome
- weight and diameter of the calluse0
- Direction
- loweste0
- Comparator
- untreate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatment of 7000 p.p.m. salts gave the lowest weight and diameter of the callus, the lowest number and length of in vitro shoots ; the lowest number and length of in vitro roots from in vitro shoots and the lowest growth of in vitro embryos compared with untreat.”
t0“DATE PALM”
Claim · model-written summary
At 7000 p.p.m. salts, in vitro shoot number and length were lowest.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- 7000 p.p.m. saltse0
- Outcome
- number and length of in vitro shootse0
- Direction
- loweste0
- Comparator
- untreate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatment of 7000 p.p.m. salts gave the lowest weight and diameter of the callus, the lowest number and length of in vitro shoots ; the lowest number and length of in vitro roots from in vitro shoots and the lowest growth of in vitro embryos compared with untreat.”
t0“DATE PALM”
Claim · model-written summary
At 7000 p.p.m. salts, root number and length were lowest.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- 7000 p.p.m. saltse0
- Outcome
- number and length of in vitro rootse0
- Direction
- loweste0
- Comparator
- untreate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatment of 7000 p.p.m. salts gave the lowest weight and diameter of the callus, the lowest number and length of in vitro shoots ; the lowest number and length of in vitro roots from in vitro shoots and the lowest growth of in vitro embryos compared with untreat.”
t0“DATE PALM”
Claim · model-written summary
At 7000 p.p.m. salts, in vitro embryo growth was lowest.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- 7000 p.p.m. saltse0
- Outcome
- growth of in vitro embryose0
- Direction
- loweste0
- Comparator
- untreate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatment of 7000 p.p.m. salts gave the lowest weight and diameter of the callus, the lowest number and length of in vitro shoots ; the lowest number and length of in vitro roots from in vitro shoots and the lowest growth of in vitro embryos compared with untreat.”
t0“DATE PALM”
Claim · model-written summary
Irrigation at 8000,9000 and 10000 ppm salts gave the worst acclimatization growth versus water.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- irrigation with solutions of 8000,9000 and 10000 ppm. saltse0
- Outcome
- growth parameterse0
- Direction
- woreste0
- Comparator
- irrigalion with walere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“irrigation with solutions of 8000,9000 and 10000 ppm. salts gave the worest results (growth parameters) in the acclimatization stage for the two studied cultivars compared with the irrigalion with waler (control or untreat)”
t0“DATE PALM”
Claim · model-written summary
Growth parameters were inversely related to salt concentration in vitro and in vivo.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Treatment
- concentration of the saltse0
- Outcome
- growth parameterse0
- Direction
- reversely proportionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were reversely proportional between concentration of the salts and the growth parameters of the two studied cuftivars in either in vitro or in vivo conditions”
t0“DATE PALM”
Claim · model-written summary
Zaghloul had more proline and greater saline tolerance than Samani, with a positive relation between proline and tolerance.
- Taxon
- DATE PALMPhoenix dactyliferat0
- Compound
- prolinee0
- Outcome
- tolerant to either in vitro or in vivo saline conditionse0
- Direction
- moree0
- Comparator
- Saman!e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zagh/cul cultivar had more proline content than Saman! cultivar and exhibited more tolerant to either in vitro or in vivo saline conditions which reflected positive relationship between (he proline contents and the tolerant to saline conditions in the two cullivars”
t0“DATE PALM”
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