Abstract
Mounting evidence has indicated that beneficial rhizobacteria can suppress foliar pathogen invasion via elicitation of induced systemic resistance (ISR). However, it remains elusive whether long non-coding RNAs (lncRNAs) are involved in the mediation of the rhizobacteria-primed ISR processes in plants. Herein, we demonstrated the ability of the rhizobacterial strain Bacillus subtilis SL18r to trigger ISR in tomato plants against the foliar pathogen Botrytis cinerea . Comparative transcriptome analysis was conducted to screen differentially expressed lncRNAs (DELs) between the non-inoculated and SL18r-inoculated plants. Among these DELs, four variants of MSTRG18363 possessed conserved binding sites for miR1918, which negatively regulates immune systems in tomato plants. The expression of MSTRG18363 in tomato leaves was significantly induced by SL18r inoculation. The transcription of MSTRG18363 was negatively correlated with the expression of miR1918, but displayed a positive correlation with the transcription of the RING-H2 finger gene SlATL20 (a target gene of miR1918). Moreover, MSTRG18363-overexpressing plants exhibited the enhanced disease resistance, reduction of miR1918 transcripts, and marked increases of SlATL20 expression. However, the SL18r-induced disease resistance was largely impaired in the MSTRG18363-silenced plants. VIGS-mediated SlATL20 silencing also greatly weakened the SL18r-induced disease resistance. Collectively, our results suggested that induction of MSTRG18363 expression in tomato plants by SL18r was conducive to promoting the decoy of miR1918 and regulating the expression of SlATL20 , thereby provoking the ISR responses against foliar pathogen infection.
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Claims 11
Claim · model-written summary
Bacillus subtilis SL18r triggered systemic resistance against Botrytis cinerea in tomato.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- Bacillus subtilis SL18re0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Second interactor
- Bacillus subtilis SL18r
Bacillus subtilise0biocontrol_agent - Outcome
- ISRe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus subtilis SL18r to trigger ISR in tomato plants against the foliar pathogen Botrytis cinerea”
t0“Tomato”
Claim · model-written summary
Four MSTRG18363 variants had conserved miR1918 binding sites.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- MSTRG18363e0
- Outcome
- conserved binding sites for miR1918e0
- Value
- four variantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four variants of MSTRG18363 possessed conserved binding sites for miR1918”
t0“Tomato”
Claim · model-written summary
SL18r inoculation significantly induced tomato leaf MSTRG18363 expression.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- leavese0
- Treatment
- SL18r inoculatione0
- Interactor
- SL18re0biocontrol_agent
- Gene
- MSTRG18363e0
- Outcome
- expression of MSTRG18363e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of MSTRG18363 in tomato leaves was significantly induced by SL18r inoculation”
t0“Tomato”
Claim · model-written summary
MSTRG18363 transcription was negatively correlated with miR1918 expression.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- miR1918e0
- Outcome
- transcription of MSTRG18363e0
- Direction
- negatively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcription of MSTRG18363 was negatively correlated with the expression of miR1918”
t0“Tomato”
Claim · model-written summary
MSTRG18363 transcription positively correlated with SlATL20 transcription.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- SlATL20e0
- Outcome
- transcription of the RING-H2 finger gene SlATL20e0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“displayed a positive correlation with the transcription of the RING-H2 finger gene SlATL20”
t0“Tomato”
Claim · model-written summary
MSTRG18363 overexpression enhanced disease resistance.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- MSTRG18363-overexpressinge0
- Outcome
- disease resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSTRG18363-overexpressing plants exhibited the enhanced disease resistance”
t0“Tomato”
Claim · model-written summary
MSTRG18363 overexpression reduced miR1918 transcripts.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- MSTRG18363-overexpressinge0
- Gene
- miR1918e0
- Outcome
- miR1918 transcriptse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSTRG18363-overexpressing plants exhibited the enhanced disease resistance, reduction of miR1918 transcripts”
t0“Tomato”
Claim · model-written summary
MSTRG18363 overexpression markedly increased SlATL20 expression.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- MSTRG18363-overexpressinge0
- Gene
- SlATL20e0
- Outcome
- SlATL20 expressione0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSTRG18363-overexpressing plants exhibited the enhanced disease resistance, reduction of miR1918 transcripts, and marked increases of SlATL20 expression”
t0“Tomato”
Claim · model-written summary
MSTRG18363 silencing largely impaired SL18r induced resistance.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- MSTRG18363-silencede0
- Gene
- MSTRG18363e0
- Outcome
- SL18r-induced disease resistancee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SL18r-induced disease resistance was largely impaired in the MSTRG18363-silenced plants”
t0“Tomato”
Claim · model-written summary
SlATL20 silencing greatly weakened SL18r induced resistance.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- SlATL20 silencinge0
- Gene
- SlATL20e0
- Outcome
- SL18r-induced disease resistancee0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlATL20 silencing also greatly weakened the SL18r-induced disease resistance”
t0“Tomato”
Claim · model-written summary
Authors propose MSTRG18363 induction promotes miR1918 decoy and regulates SlATL20 expression to provoke resistance.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- MSTRG18363e0
- Outcome
- ISR responses against foliar pathogen infectione0
- Qualifier
- promoting the decoy of miR1918 and regulating the expression of SlATL20e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction of MSTRG18363 expression in tomato plants by SL18r was conducive to promoting the decoy of miR1918 and regulating the expression of SlATL20 , thereby provoking the ISR responses against foliar pathogen infection”
t0“Tomato”
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