Abstract
The impact of nano silver & nano chitosan particles on sterilization, nano Fe and Zn on callus formation of immature inflorescence of date palm cv. Barhee during the establishment stage was investigated with immersion and adding to MS culture medium.Thelowest total contamination percentage and the highest survival percentage were achieved with nano silver particles at200 mg/l, and nano chitosan at 150, and 200 mg/l. The lowest contamination %recorded in medium culture containing silver nanoparticles at 4mg/l with NAA at 100mg/l and chitosan nanoparticles at 4 mg/l with 2,4-D at 100 mg/l. The optimum callus formation percentage and callus size were obtained on MS medium supplemented with picloram at 8 mg/l. The highest callus weight and size were showed with NAA at 10 mg/l, 2ip at 6mg/l & Kin at 6 mg/l during callus proliferation. In multiplication stage, the highest number of shoot / culture were occurred on MS medium culture supplemented with Fe nano particles at 20.8 mg/l , MS medium culture supplemented by Fe nano particles at 27.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significant differences among them. The highest average shoot length (cm) was obtained with MS medium containing Fe nano particles at 27.8 mg/l, MS medium supplemented by Fe nano particles at 20.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significantly differences among them. Interaction between cytokinins and auxin concentration, indicated, the highest number of shoots / culture were achieved with NAA at 2.0 mg/l, 2ip at 4 mg/l, kin at 4mg/l during the 1 st, 2 nd & 3 rd subcultures, respectively. The highest rooting percentage and number of roots/ microshoots were obtained with MS containing NAA at 0.5 mg/l. The highest survival percentages in acclimatization stage were occurred with medium mixtures of sand: peat: vermiculite: perlite at (1:2: 1:1) and (2: 1: 1:1), respectively.
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Claims 14
Claim · model-written summary
Nano silver at 200 mg/l and nano chitosan at 150 or 200 mg/l gave the lowest contamination and highest survival.
- Taxon
- date palm
Phoenix dactyliferat0 - Material
- cv. Barheet0
- Treatment
- nano silver particles at200 mg/le0
- Outcome
- total contamination percentagee0
- Direction
- loweste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“Thelowest total contamination percentage and the highest survival percentage were achieved with nano silver particles at200 mg/l, and nano chitosan at 150, and 200 mg/l.”
Claim · model-written summary
Nano chitosan at 150 and 200 mg/l gave high survival.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- nano chitosan at 150, and 200 mg/le0
- Outcome
- survival percentagee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“Thelowest total contamination percentage and the highest survival percentage were achieved with nano silver particles at200 mg/l, and nano chitosan at 150, and 200 mg/l.”
Claim · model-written summary
Silver nanoparticles with NAA gave the lowest culture contamination.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- silver nanoparticles at 4mg/l with NAA at 100mg/le0
- Outcome
- contamination %e0
- Direction
- loweste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The lowest contamination %recorded in medium culture containing silver nanoparticles at 4mg/l with NAA at 100mg/l and chitosan nanoparticles at 4 mg/l with 2,4-D at 100 mg/l.”
Claim · model-written summary
Chitosan nanoparticles with 2,4-D gave the lowest culture contamination.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- chitosan nanoparticles at 4 mg/l with 2,4-D at 100 mg/le0
- Outcome
- contamination %e0
- Direction
- loweste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The lowest contamination %recorded in medium culture containing silver nanoparticles at 4mg/l with NAA at 100mg/l and chitosan nanoparticles at 4 mg/l with 2,4-D at 100 mg/l.”
Claim · model-written summary
Picloram at 8 mg/l gave optimal callus formation and size.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- picloram at 8 mg/le0
- Outcome
- callus formation percentage and callus sizee0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The optimum callus formation percentage and callus size were obtained on MS medium supplemented with picloram at 8 mg/l.”
Claim · model-written summary
NAA, 2ip and Kin gave the highest callus weight and size during proliferation.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Organ / stage
- callus proliferatione0
- Treatment
- NAA at 10 mg/l, 2ip at 6mg/l & Kin at 6 mg/le0
- Outcome
- callus weight and sizee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The highest callus weight and size were showed with NAA at 10 mg/l, 2ip at 6mg/l & Kin at 6 mg/l during callus proliferation.”
Claim · model-written summary
Fe nanoparticles at 20.8 mg/l gave a highest shoot number in the first subculture.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Organ / stage
- first subculturee0
- Treatment
- Fe nano particles at 20.8 mg/le0
- Outcome
- number of shoot / culturee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“the highest number of shoot / culture were occurred on MS medium culture supplemented with Fe nano particles at 20.8 mg/l , MS medium culture supplemented by Fe nano particles at 27.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significant differences among them.”
Claim · model-written summary
Fe and Zn nanoparticles gave a highest shoot number in the first subculture.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- Fe nano particles at 27.8 mg/l and Zn nano particles at 4.3 mg/le0
- Outcome
- number of shoot / culturee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“the highest number of shoot / culture were occurred on MS medium culture supplemented with Fe nano particles at 20.8 mg/l , MS medium culture supplemented by Fe nano particles at 27.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significant differences among them.”
Claim · model-written summary
Fe nanoparticles at 27.8 mg/l gave the highest average shoot length.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- Fe nano particles at 27.8 mg/le0
- Outcome
- average shoot length (cm)e0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The highest average shoot length (cm) was obtained with MS medium containing Fe nano particles at 27.8 mg/l, MS medium supplemented by Fe nano particles at 20.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significantly differences among them.”
Claim · model-written summary
Fe and Zn nanoparticles gave the highest average shoot length.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- Fe nano particles at 20.8 mg/l and Zn nano particles at 4.3 mg/le0
- Outcome
- average shoot length (cm)e0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The highest average shoot length (cm) was obtained with MS medium containing Fe nano particles at 27.8 mg/l, MS medium supplemented by Fe nano particles at 20.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significantly differences among them.”
Claim · model-written summary
NAA, 2ip and kin yielded the most shoots across the three subcultures.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- NAA at 2.0 mg/l, 2ip at 4 mg/l, kin at 4mg/le0
- Outcome
- number of shoots / culturee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“the highest number of shoots / culture were achieved with NAA at 2.0 mg/l, 2ip at 4 mg/l, kin at 4mg/l during the 1 st, 2 nd & 3 rd subcultures, respectively.”
Claim · model-written summary
NAA at 0.5 mg/l gave the highest rooting percentage and roots per microshoot.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- NAA at 0.5 mg/le0
- Outcome
- rooting percentage and number of roots/ microshootse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The highest rooting percentage and number of roots/ microshoots were obtained with MS containing NAA at 0.5 mg/l.”
Claim · model-written summary
The first sand, peat, vermiculite and perlite mixture gave the highest acclimatization survival.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- sand: peat: vermiculite: perlite at (1:2: 1:1)e0
- Outcome
- survival percentagese0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The highest survival percentages in acclimatization stage were occurred with medium mixtures of sand: peat: vermiculite: perlite at (1:2: 1:1) and (2: 1: 1:1), respectively.”
Claim · model-written summary
The second sand, peat, vermiculite and perlite mixture gave the highest acclimatization survival.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- cv. Barheet0
- Treatment
- sand: peat: vermiculite: perlite at (1:2: 1:1) and (2: 1: 1:1)e0
- Outcome
- survival percentagese0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“date palm cv. Barhee”
e0“The highest survival percentages in acclimatization stage were occurred with medium mixtures of sand: peat: vermiculite: perlite at (1:2: 1:1) and (2: 1: 1:1), respectively.”
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