Abstract
Tthe desirable cultivars of date palm (Zaghloul and Samany) beginning from segments of fruits flesh to obtain initial callus, compact callus and meristematic center cells. For this purpose, a study was conducted to define micropropagation protocol based on the cell totipotency to obtains formation of callus. Sodium hypochlorite was very effective for surface sterilization of explants. The best method of surface sterilization was achieved by using treatment containing NaOCl 70% that produced the lowest percentage of contamination 1.33% for Zaghloul and 1.58% for Samany explants by time expanding during sterilization period from 10 to 30 mint. It was observed that the highest percentage of callus was produced at Khalal stage than other stages of fruit development of Zaghloul and Samany cv., when culturing fruit flesh in MS medium containing 100mg/1 of 2,4-D or 30mg/l of Picloram (Pic). The effect of physiological stage of fruit and growth regulators on callus browning was investigated. The results indicates that the treatment supplemented with Pic (30mg/l) produced the highest amount of total soluble phenols in callus 3.73 mg/100g F.W for Zaghloul at Rutab stage and 3.42 mg/100g F.W for Samany at kimri stage. On the other hand, there were significant differences between all stages on total soluble phenols of callus in Zaghloul. While there was no any significant difference among Khalal and Rutab stage on callus formation from Samany explants. The stages of initial callus formation and callus percentages of date palm (Phoenix dactylifera L.) were studied from the 2-10 months-old cultures in MS medium containing 2,4-D or Pic at different concentrations. The study revealed that the compact callus was formed from the friable callus and meristematic center cells. The number of these center cells were unequally distributed inside the compact callus.
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Claims 10
Claim · model-written summary
NaOCl 70% achieved 1.33% contamination for Zaghloul explants.
- Taxon
- date palm
Phoenix dactyliferat0 - Material
- Zaghloule0
- Treatment
- NaOCl 70%e0
- Outcome
- contaminatione0
- Direction
- loweste0
- Value
- 1.33%e0
- Qualifier
- from 10 to 30 minte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best method of surface sterilization was achieved by using treatment containing NaOCl 70% that produced the lowest percentage of contamination 1.33% for Zaghloul and 1.58% for Samany explants by time expanding during sterilization period from 10 to 30 mint”
t0“Date Palm”
Claim · model-written summary
NaOCl 70% achieved 1.58% contamination for Samany explants.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Samanye0
- Treatment
- NaOCl 70%e0
- Outcome
- contaminatione0
- Direction
- loweste0
- Value
- 1.58%e0
- Qualifier
- from 10 to 30 minte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best method of surface sterilization was achieved by using treatment containing NaOCl 70% that produced the lowest percentage of contamination 1.33% for Zaghloul and 1.58% for Samany explants by time expanding during sterilization period from 10 to 30 mint”
t0“Date Palm”
Claim · model-written summary
Zaghloul fruit flesh at Khalal stage gave the highest callus percentage in the stated media.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Zaghloule0
- Organ / stage
- Khalal stagee0
- Treatment
- 100mg/1 of 2,4-D or 30mg/l of Picloram (Pic)e0
- Outcome
- percentage of calluse0
- Direction
- higheste0
- Comparator
- other stages of fruit developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest percentage of callus was produced at Khalal stage than other stages of fruit development of Zaghloul and Samany cv., when culturing fruit flesh in MS medium containing 100mg/1 of 2,4-D or 30mg/l of Picloram (Pic)”
t0“Date Palm”
Claim · model-written summary
Samany fruit flesh at Khalal stage gave the highest callus percentage in the stated media.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Samanye0
- Organ / stage
- Khalal stagee0
- Treatment
- 100mg/1 of 2,4-D or 30mg/l of Picloram (Pic)e0
- Outcome
- percentage of calluse0
- Direction
- higheste0
- Comparator
- other stages of fruit developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest percentage of callus was produced at Khalal stage than other stages of fruit development of Zaghloul and Samany cv., when culturing fruit flesh in MS medium containing 100mg/1 of 2,4-D or 30mg/l of Picloram (Pic)”
t0“Date Palm”
Claim · model-written summary
Pic 30mg/l produced 3.73 mg/100g F.W total soluble phenols in Zaghloul callus at Rutab stage.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Zaghloule0
- Organ / stage
- Rutab stagee0
- Treatment
- Pic (30mg/l)e0
- Outcome
- total soluble phenols in calluse0
- Direction
- higheste0
- Value
- 3.73 mg/100g F.We0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the treatment supplemented with Pic (30mg/l) produced the highest amount of total soluble phenols in callus 3.73 mg/100g F.W for Zaghloul at Rutab stage and 3.42 mg/100g F.W for Samany at kimri stage”
t0“Date Palm”
Claim · model-written summary
Pic 30mg/l produced 3.42 mg/100g F.W total soluble phenols in Samany callus at kimri stage.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Samanye0
- Organ / stage
- kimri stagee0
- Treatment
- Pic (30mg/l)e0
- Outcome
- total soluble phenols in calluse0
- Direction
- higheste0
- Value
- 3.42 mg/100g F.We0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the treatment supplemented with Pic (30mg/l) produced the highest amount of total soluble phenols in callus 3.73 mg/100g F.W for Zaghloul at Rutab stage and 3.42 mg/100g F.W for Samany at kimri stage”
t0“Date Palm”
Claim · model-written summary
Total soluble phenols in Zaghloul callus differed significantly among developmental stages.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Zaghloule0
- Organ / stage
- all stagese0
- Outcome
- total soluble phenols of calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were significant differences between all stages on total soluble phenols of callus in Zaghloul”
t0“Date Palm”
Claim · model-written summary
Samany callus formation did not differ significantly between Khalal and Rutab stages.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Samanye0
- Organ / stage
- Khalal and Rutab stagee0
- Outcome
- callus formatione0
- Direction
- no any significant differencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no any significant difference among Khalal and Rutab stage on callus formation from Samany explants”
t0“Date Palm”
Claim · model-written summary
Compact callus formed from friable callus and meristematic center cells.
- Taxon
- date palmPhoenix dactyliferat0
- Outcome
- compact callus was formed from the friable callus and meristematic center cellse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the compact callus was formed from the friable callus and meristematic center cells”
t0“Date Palm”
Claim · model-written summary
Meristematic center cells were unequally distributed in compact callus.
- Taxon
- date palmPhoenix dactyliferat0
- Outcome
- center cells were unequally distributed inside the compact calluse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of these center cells were unequally distributed inside the compact callus”
t0“Date Palm”
The same questions in other papers
2,4-D · growth · Phoenix dactylifera (date palm)Positive and negative results
21 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
21 papers more in this group
See all 25 papers →picloram · growth · Phoenix dactylifera (date palm)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.