Paper · 2022 · PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

SUPERIORITY OF NANO-SILVER NITRATE AND NANO-CHITOSAN IN CONTROLLING BACTERIAL CONTAMINATION AND PROMOTING GROWTH OF In vitro DATE PALM CULTURES

LAMIAA ABOGARRA; NAWAL EISA; GEHAD EL-HABBAA; et al.

14
claims
27
verbatim quotes
5
citations
4
research areas
✓
Open access

Abstract

Date palm (Phoenix dactylifera L.) a perennial monocotyledonous tree it’s have an ecological importance and economical role in several countries of Middle East and North Africa, date palm can’t be propagate by seeds, meanwhile the offshoots produced low numbers of offshoots for the given period, so tissue culture technique become a great important for produced the huge demand plantlets, the in vitro technique facing a problem as the contamination by bacterial and fungus that lead to loss the cultures, for eliminate this problem many treatments were used as chitosan 5 and 15 ml/l, AgNO3 4.0 and 7.0 ml/l, Zn 4.0 and 7.0 mg/l and cu 0.5 and 1.0 mg/l. as the main forms or as a nanoparticles NPs in addition to streptomycin 40 and 50 mg/l and AgNO3 + Sodium thiosulfate 0.5 and 1.0 ml/l were added to rooting stage medium for 3 subcultures for each, then these plantlets were acclimatized under greenhouse condition. Collected data were subjected to analysis of variance (ANOVA) and the means were compared following t-test using LSD values at 5%. The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced, while the treatments Zn and Cu at two tested levels produced 11% contamination, the observation of the vegetative growth and leaves contents of indoles and amino acids mg/g f.w. were found highest values under chitosan and AgNO3 and the combination in between as the main forms or as NPs treatments, as well as, the acclimatization stage presented the great survival percentage % under Chitosan and AgNO3 and combination in between as the main form or as NPs. The present study have been suggest that NPs chitosan, AgNO3 and the combination in between might be a promising method for reduced or eliminate the in vitro microbes, reduced the harmful effects on the valuable date palm plantlets and increasing growth parameters.

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Claims 14

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan yielded zero contamination of date palm cultures at all tested levels.

Taxon
Date palmPhoenix dactyliferat0
Treatment
chitosane0
Outcome
cultures contaminatione0
Direction
zeroe0
Value
zero %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced”

✓ located verbatim in the source · abstract, characters 1052–1289 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Silver nitrate yielded zero contamination of date palm cultures.

Taxon
Date palmPhoenix dactyliferat0
Treatment
silver nitrate AgNO3e0
Outcome
cultures contaminatione0
Direction
zeroe0
Value
zero %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced”

✓ located verbatim in the source · abstract, characters 1052–1289 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan plus AgNO3 yielded zero contamination.

Taxon
Date palmPhoenix dactyliferat0
Treatment
Chitosan + AgNO3e0
Outcome
cultures contaminatione0
Direction
zeroe0
Value
zero %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced”

✓ located verbatim in the source · abstract, characters 1052–1289 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan plus Zn yielded zero contamination.

Taxon
Date palmPhoenix dactyliferat0
Treatment
chitosan + Zne0
Outcome
cultures contaminatione0
Direction
zeroe0
Value
zero %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced”

✓ located verbatim in the source · abstract, characters 1052–1289 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan plus Cu yielded zero contamination.

Taxon
Date palmPhoenix dactyliferat0
Treatment
chitosan + Cue0
Outcome
cultures contaminatione0
Direction
zeroe0
Value
zero %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The percentage of cultures contamination was to be found zero % with the treatments chitosan, silver nitrate AgNO3, in addition to the combination of Chitosan + AgNO3, chitosan + Zn and finally chitosan + Cu at all tested levels produced”

✓ located verbatim in the source · abstract, characters 1052–1289 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Zn treatments produced 11% contamination.

Taxon
Date palmPhoenix dactyliferat0
Treatment
Zne0
Outcome
contaminatione0
Value
11%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“while the treatments Zn and Cu at two tested levels produced 11% contamination”

✓ located verbatim in the source · abstract, characters 1291–1369 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Cu treatments produced 11% contamination.

Taxon
Date palmPhoenix dactyliferat0
Treatment
Cue0
Outcome
contaminatione0
Value
11%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“while the treatments Zn and Cu at two tested levels produced 11% contamination”

✓ located verbatim in the source · abstract, characters 1291–1369 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan gave highest vegetative growth and leaf indole and amino acid contents.

Taxon
Date palmPhoenix dactyliferat0
Treatment
chitosane0
Outcome
vegetative growth and leaves contents of indoles and amino acidse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the observation of the vegetative growth and leaves contents of indoles and amino acids mg/g f.w. were found highest values under chitosan and AgNO3 and the combination in between as the main forms or as NPs treatments”

✓ located verbatim in the source · abstract, characters 1371–1589 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNO3 gave highest vegetative growth and leaf indole and amino acid contents.

Taxon
Date palmPhoenix dactyliferat0
Treatment
AgNO3e0
Outcome
vegetative growth and leaves contents of indoles and amino acidse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the observation of the vegetative growth and leaves contents of indoles and amino acids mg/g f.w. were found highest values under chitosan and AgNO3 and the combination in between as the main forms or as NPs treatments”

✓ located verbatim in the source · abstract, characters 1371–1589 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan plus AgNO3 gave highest vegetative growth and leaf indole and amino acid contents.

Taxon
Date palmPhoenix dactyliferat0
Treatment
combination in betweene0
Outcome
vegetative growth and leaves contents of indoles and amino acidse0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the observation of the vegetative growth and leaves contents of indoles and amino acids mg/g f.w. were found highest values under chitosan and AgNO3 and the combination in between as the main forms or as NPs treatments”

✓ located verbatim in the source · abstract, characters 1371–1589 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan yielded high acclimatization survival.

Taxon
Date palmPhoenix dactyliferat0
Treatment
Chitosane0
Outcome
survival percentagee0
Direction
greate0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the acclimatization stage presented the great survival percentage % under Chitosan and AgNO3 and combination in between as the main form or as NPs”

✓ located verbatim in the source · abstract, characters 1603–1749 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

AgNO3 yielded high acclimatization survival.

Taxon
Date palmPhoenix dactyliferat0
Treatment
AgNO3e0
Outcome
survival percentagee0
Direction
greate0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the acclimatization stage presented the great survival percentage % under Chitosan and AgNO3 and combination in between as the main form or as NPs”

✓ located verbatim in the source · abstract, characters 1603–1749 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chitosan plus AgNO3 yielded high acclimatization survival.

Taxon
Date palmPhoenix dactyliferat0
Treatment
combination in betweene0
Outcome
survival percentagee0
Direction
greate0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the acclimatization stage presented the great survival percentage % under Chitosan and AgNO3 and combination in between as the main form or as NPs”

✓ located verbatim in the source · abstract, characters 1603–1749 · show in text ↑
t0

“DATE PALM”

✓ located verbatim in the source · title, characters 125–134 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggest nanoparticle chitosan, AgNO3, and their combination to reduce microbes and improve plantlet growth.

Taxon
date palmPhoenix dactyliferae0
Outcome
reduced or eliminate the in vitro microbes, reduced the harmful effects on the valuable date palm plantlets and increasing growth parameterse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phoenix dactylifera
Phoenix dactylifera · TaxonEJ Burns · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“NPs chitosan, AgNO3 and the combination in between might be a promising method for reduced or eliminate the in vitro microbes, reduced the harmful effects on the valuable date palm plantlets and increasing growth parameters”

✓ located verbatim in the source · abstract, characters 1792–2015 · show in text ↑

chitosan · growth · Phoenix dactylifera (date palm)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

silver(1+) nitrate · growth · Phoenix dactylifera (date palm)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

chitosan · survival · Phoenix dactylifera (date palm)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.