Abstract
Abstract Seed priming is widely used in commercial seeds and its main function is to accelerate and synchronize seed germination. Undesirably, primed seeds show reduced longevity and treatments like heat shock have been shown to improve longevity in primed seeds. Nonetheless, the effect of heat shock treatment on primed seeds at the mRNA level is not known. Thus, the aim of this work was to investigate the effect of heat shock treatment on the longevity of primed tomato (Solanum lycopersicum) seeds at the physiological and transcriptome levels. Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying. Germination, vigor and longevity were evaluated. Transcriptome analysis was performed by RNA sequencing (RNA-seq) from biological samples collected immediately after priming and another samples collected from primed seeds followed by the heat shock treatments. The gene expression was validated by quantitative real time PCR (RT-qPCR). We showed that applying heat shock treatment after priming increased germination speed, enhanced seed longevity and preserved the vigor during storage of primed tomato seeds. Through transcriptome analysis, 368 differentially expressed genes were identified, from which 298 genes were up-regulated and 70 were down-regulated. We showed the increase of mRNA levels of HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genes, suggesting the involvement of the proteins coded by these transcripts in the enhancement of longevity in primed tomato seeds. The heat shock treatment after priming enhances and preserves the vigor of tomato primed seeds during storage. In addition, improves seed longevity through the increase in the expression of transcripts related to protection by response to stress.
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Claims 9
Claim · model-written summary
Heat shock after priming increased germination speed of primed tomato seeds.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- applying heat shock treatment after priminge1
- Outcome
- germination speede1
- Direction
- increasede1
- Comparator
- primed and driede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e1“We showed that applying heat shock treatment after priming increased germination speed, enhanced seed longevity and preserved the vigor during storage of primed tomato seeds.”
e2“Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying.”
Claim · model-written summary
Heat shock after priming enhanced seed longevity of primed tomato seeds.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- applying heat shock treatment after priminge1
- Outcome
- seed longevitye1
- Direction
- enhancede1
- Comparator
- primed and driede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e1“We showed that applying heat shock treatment after priming increased germination speed, enhanced seed longevity and preserved the vigor during storage of primed tomato seeds.”
e2“Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying.”
Claim · model-written summary
Heat shock after priming preserved the vigor of primed tomato seeds during storage.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- applying heat shock treatment after priminge1
- Outcome
- vigor during storagee1
- Direction
- preservede1
- Comparator
- primed and driede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e1“We showed that applying heat shock treatment after priming increased germination speed, enhanced seed longevity and preserved the vigor during storage of primed tomato seeds.”
e2“Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying.”
Claim · model-written summary
368 differentially expressed genes were identified in the transcriptome analysis.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- differentially expressed genese3
- Value
- 368e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e3“Through transcriptome analysis, 368 differentially expressed genes were identified, from which 298 genes were up-regulated and 70 were down-regulated.”
Claim · model-written summary
Heat shock up-regulated 298 genes in primed tomato seeds relative to primed and dried seeds.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- heat shock treatmente2
- Outcome
- genese3
- Direction
- up-regulatede3
- Value
- 298 genese3
- Comparator
- primed and driede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e2“Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying.”
e3“Through transcriptome analysis, 368 differentially expressed genes were identified, from which 298 genes were up-regulated and 70 were down-regulated.”
Claim · model-written summary
Heat shock down-regulated 70 genes in primed tomato seeds relative to primed and dried seeds.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- heat shock treatmente2
- Outcome
- genese3
- Direction
- down-regulatede3
- Value
- 70e3
- Comparator
- primed and driede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e2“Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying.”
e3“Through transcriptome analysis, 368 differentially expressed genes were identified, from which 298 genes were up-regulated and 70 were down-regulated.”
Claim · model-written summary
Heat shock increased mRNA levels of HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genes.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- heat shock treatmente2
- Gene
- HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genese4
- Outcome
- mRNA levelse4
- Direction
- increasee4
- Comparator
- primed and driede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e2“Tomato seeds were primed and dried (control). Alternatively, primed seeds were subjected to heat shock treatment (38 °C/32 % relative humidity) before drying.”
e4“We showed the increase of mRNA levels of HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genes, suggesting the involvement of the proteins coded by these transcripts in the enhancement of longevity in primed tomato seeds.”
Claim · model-written summary
The HSF-like and HSP-like chaperone genes are involved in enhancing seed longevity.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genese4
- Outcome
- enhancement of longevitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e4“We showed the increase of mRNA levels of HEAT SHOCK FACTOR-like and HEAT SHOCK PROTEIN-like chaperone genes, suggesting the involvement of the proteins coded by these transcripts in the enhancement of longevity in primed tomato seeds.”
Claim · model-written summary
Heat shock improves seed longevity through increased expression of transcripts related to stress protection.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- seed longevitye5
- Qualifier
- protection by response to stresse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcriptome analysis in osmo-primed tomato seeds with enhanced longevity by heat shock treatment”
e5“In addition, improves seed longevity through the increase in the expression of transcripts related to protection by response to stress.”
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