Abstract
Halophyte Lobularia maritima LmSAP encodes an A20AN1 zinc-finger stress-associated protein which expression is up-regulated by abiotic stresses and heavy metals in transgenic tobacco. To deepen our understanding of LmSAP function, we isolated a 1,147 bp genomic fragment upstream of LmSAP coding sequence designated as PrLmSAP. In silico analyses of PrLmSAP revealed the presence of consensus CAAT and TATA boxes and cis-regulatory elements required for abiotic stress, phytohormones, pathogen, and wound responses, and also for tissue-specific expression. The PrLmSAP sequence was fused to the β-glucuronidase (gusA) reporter gene and transferred to rice. Histochemical GUS staining showed a pattern of tissue-specific expression in transgenic rice, with staining observed in roots, coleoptiles, leaves, stems and floral organs but not in seeds or in the root elongation zone. Wounding strongly stimulated GUS accumulation in leaves and stems. Interestingly, we observed a high stimulation of the promoter activity when rice seedlings were exposed to NaCl, PEG, ABA, MeJA, GA, cold, and heavy metals (Al3+, Cd2+, Cu2+ and Zn2+). These results suggest that the LmSAP promoter can be a convenient tool for stress-inducible gene expression and is a potential candidate for crop genetic engineering.
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Claims 4
Claim · model-written summary
PrLmSAP contains predicted regulatory elements for stress, hormone and tissue-specific responses.
- Taxon
- Lobularia maritima
Lobularia maritimat0 - Gene
- PrLmSAPe0
- Outcome
- consensus CAAT and TATA boxes and cis-regulatory elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PrLmSAP revealed the presence of consensus CAAT and TATA boxes and cis-regulatory elements required for abiotic stress, phytohormones, pathogen, and wound responses, and also for tissue-specific expression”
t0“Lobularia maritima”
Claim · model-written summary
PrLmSAP drove tissue-specific expression in rice organs but not seeds or the root elongation zone.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- roots, coleoptiles, leaves, stems and floral organse0
- Outcome
- tissue-specific expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tissue-specific expression in transgenic rice, with staining observed in roots, coleoptiles, leaves, stems and floral organs but not in seeds or in the root elongation zone”
Claim · model-written summary
Wounding stimulated GUS accumulation in rice leaves and stems.
- Taxon
- riceOryza sativat0
- Organ / stage
- leaves and stemse0
- Treatment
- Woundinge0
- Outcome
- GUS accumulatione0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding strongly stimulated GUS accumulation in leaves and stems”
t0“transferred to rice. Histochemical GUS staining”
Claim · model-written summary
NaCl, PEG, ABA, MeJA, GA, cold and heavy metals stimulated promoter activity in rice seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Treatment
- NaCl, PEG, ABA, MeJA, GA, cold, and heavy metals (Al3+, Cd2+, Cu2+ and Zn2+)e0
- Outcome
- promoter activitye0
- Direction
- stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high stimulation of the promoter activity when rice seedlings were exposed to NaCl, PEG, ABA, MeJA, GA, cold, and heavy metals (Al3+, Cd2+, Cu2+ and Zn2+)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.