Abstract
Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex.
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Claims 11
Claim · model-written summary
Knockout of OsFRDL1 resulted in leaf chlorosis compared with wild-type rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- Knockout of this genee2
- Gene
- OsFRDL1e0
- Outcome
- leaf chlorosise2
- Direction
- resulted ine2
- Comparator
- wild-type ricee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e2“Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele”
e3“The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice”
Claim · model-written summary
Knockout of OsFRDL1 lowered leaf Fe concentration compared with wild-type rice.
- Taxon
- riceOryza sativae0
- Treatment
- Knockout of this genee2
- Outcome
- leaf Fe concentratione2
- Direction
- lowere2
- Comparator
- wild-type ricee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e2“Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele”
e3“The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice”
Claim · model-written summary
Knockout of OsFRDL1 raised zinc and manganese concentrations in the leaves compared with wild-type rice.
- Taxon
- riceOryza sativae0
- Treatment
- Knockout of this genee2
- Outcome
- zinc and manganese concentration in the leavese2
- Direction
- higher accumulatione2
- Comparator
- wild-type ricee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e2“Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele”
e3“The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice”
Claim · model-written summary
Knockout of OsFRDL1 causes precipitation of Fe in the root stele.
- Taxon
- riceOryza sativae0
- Treatment
- Knockout of this genee2
- Outcome
- precipitation of Fe in the root's stelee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e2“Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele”
Claim · model-written summary
The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line than in wild-type rice.
- Taxon
- riceOryza sativae0
- Treatment
- knockout linee3
- Outcome
- concentration of citrate and ferric iron in the xylem sape3
- Direction
- lowere3
- Comparator
- wild-type ricee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e3“The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice”
Claim · model-written summary
Heterologous expression of OsFRDL1 in Xenopus oocytes showed citrate transport activity.
- Taxon
- riceOryza sativae0
- Treatment
- Heterologous expression of OsFRDL1 in Xenopus oocytese4
- Gene
- OsFRDL1e4
- Outcome
- transport activity for citratee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e4“Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate”
Claim · model-written summary
OsFRDL1 is localized at the pericycle cells of rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- pericycle cells of the rootse5
- Gene
- OsFRDL1e5
- Outcome
- localizede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e5“Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots”
Claim · model-written summary
Knockout did not change Al-induced citrate secretion from the roots compared with wild-type rice.
- Taxon
- riceOryza sativae0
- Treatment
- knockout linee6
- Outcome
- secretion of citrate from the rootse6
- Direction
- no differencee6
- Qualifier
- Al-inducede6
- Comparator
- wild-type ricee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e6“On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice”
Claim · model-written summary
OsFRDL1 expression level was not affected by Fe deficiency or Al toxicity.
- Taxon
- riceOryza sativae0
- Treatment
- Fe deficiency or Al toxicitye1
- Gene
- OsFRDL1e0
- Outcome
- expression levele1
- Direction
- not affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e1“This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity”
Claim · model-written summary
OsFRDL1 is mainly expressed in rice roots.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse1
- Gene
- OsFRDL1e0
- Outcome
- mainly expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e1“This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity”
Claim · model-written summary
OsFRDL1 is a citrate transporter needed for efficient translocation of Fe to the shoot as a Fe-citrate complex.
- Taxon
- riceOryza sativae0
- Gene
- OsFRDL1e7
- Compound
- citratee7
- Outcome
- translocation of Fe to the shoote7
- Qualifier
- as a Fe-citrate complexe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1)”
e7“Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex”
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