Abstract
MicroRNAs (miRNAs) are 21-24-nucleotide-long RNAs that function as ubiquitous post-transcriptional regulators of gene expression in plants and animals. Increasing evidence points to the important role of miRNAs in plant responses to abiotic and biotic stresses. Cadmium (Cd) is a nonessential heavy metal highly toxic to plants. Although many genes encoding metal transporters have been characterized, the mechanisms for the regulation of the expression of the heavy-metal transporter genes are largely unknown. In this study, we found that the expression of miR268 in rice was significantly induced under Cd stress. By contrast, expression of natural resistance-associated macrophage protein 3 (NRAMP3), a target gene of miR268, was dramatically decreased by Cd treatment. Overexpression of miR268 inhibited rice seedling growth under Cd stress. The transgenic miR268-overexpressing plant leaves contained increased levels of hydrogen peroxide and malondialdehyde, and their seedlings accumulated increased levels of Cd when compared to those in wild-type plants. These results indicate that miR268 acts as a negative regulator of rice's tolerance to Cd stress. Thus, miRNA-guided regulation of gene expression plays an important role in plant responses to heavy-metal stress.
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Claims 7
Claim · model-written summary
Cadmium stress significantly induced rice miR268 expression.
- Taxon
- rice
Oryza sativae0 - Treatment
- Cd stresse0
- Gene
- miR268e0
- Outcome
- expression of miR268e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of miR268 in rice was significantly induced under Cd stress”
Claim · model-written summary
Cadmium treatment dramatically decreased rice NRAMP3 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- Cd treatmente0
- Gene
- natural resistance-associated macrophage protein 3 (NRAMP3)e0
- Outcome
- expression of natural resistance-associated macrophage protein 3 (NRAMP3)e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of natural resistance-associated macrophage protein 3 (NRAMP3), a target gene of miR268, was dramatically decreased by Cd treatment”
t0“Rice”
Claim · model-written summary
Overexpressing miR268 inhibited rice seedling growth under cadmium stress.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of miR268e0
- Gene
- miR268e0
- Outcome
- rice seedling growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of miR268 inhibited rice seedling growth under Cd stress”
Claim · model-written summary
miR268 overexpression increased leaf hydrogen peroxide in rice relative to wild type.
- Taxon
- RiceOryza sativat0
- Organ / stage
- leavese0
- Treatment
- miR268-overexpressinge0
- Outcome
- levels of hydrogen peroxidee0
- Direction
- increasede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic miR268-overexpressing plant leaves contained increased levels of hydrogen peroxide and malondialdehyde, and their seedlings accumulated increased levels of Cd when compared to those in wild-type plants”
t0“Rice”
Claim · model-written summary
miR268 overexpression increased leaf malondialdehyde in rice relative to wild type.
- Taxon
- RiceOryza sativat0
- Organ / stage
- leavese0
- Treatment
- miR268-overexpressinge0
- Outcome
- malondialdehydee0
- Direction
- increasede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic miR268-overexpressing plant leaves contained increased levels of hydrogen peroxide and malondialdehyde, and their seedlings accumulated increased levels of Cd when compared to those in wild-type plants”
t0“Rice”
Claim · model-written summary
miR268 overexpression increased seedling cadmium accumulation relative to wild type.
- Taxon
- RiceOryza sativat0
- Organ / stage
- seedlingse0
- Outcome
- levels of Cde0
- Direction
- increasede0
- Comparator
- wild-type plantse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their seedlings accumulated increased levels of Cd when compared to those in wild-type plants”
t0“Rice”
Claim · model-written summary
miR268 acts as a negative regulator of rice tolerance to cadmium stress.
- Taxon
- riceOryza sativae0
- Gene
- miR268e0
- Outcome
- tolerance to Cd stresse0
- Qualifier
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicate that miR268 acts as a negative regulator of rice's tolerance to Cd stress”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.