Abstract
Plasma membrane NADPH oxidases are major producers of reactive oxygen species (ROS) in plant cells under normal growth and stress conditions. In the present study the total activity of rice NADPH oxidases and the transcription of OsRbohA, which encodes an Oryza sativa plasma membrane NADPH oxidase, were stimulated by drought. OsRbohA was expressed in all tissues examined throughout development. Its mRNA was upregulated by a number of factors, including heat, drought, salt, oxidative stress and methyl jasmonate treatment. Compared with wild-type (WT), the OsRbohA-knockout mutant osrbohA exhibited upregulated expression of other respiratory burst oxidase homolog genes and multiple abnormal agronomic traits, including reduced biomass, low germination rate and decreased pollen viability and seed fertility. However, OsRbohA-overexpressing transgenic plants showed no differences in these traits compared with WT. Although osrbohA leaves and roots produced more ROS than WT, the mutant had lesser intracellular ROS. In contrast, OsRbohA-overexpressing transgenic plants exhibited higher ROS production at the intracellular level and in tissues. Ablation of OsRbohA impaired the tolerance of plants to various water stresses, whereas its overexpression enhanced the tolerance. In addition, a number of genes related to energy supply, substrate transport, stress response and transcriptional regulation were differentially expressed in osrbohA plants even under normal growth conditions, suggesting that OsRbohA has fundamental and broad functions in rice. These results indicate that OsRbohA-mediated processes are governed by complex signaling pathways that function during the developmental regulation and drought-stress response in rice.
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Claims 14
Claim · model-written summary
Drought stimulated total NADPH oxidase activity and OsRbohA transcription.
- Taxon
- rice
Oryza sativae0 - Treatment
- droughte0
- Gene
- OsRbohAe0
- Outcome
- total activity of rice NADPH oxidases and the transcription of OsRbohAe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the total activity of rice NADPH oxidases and the transcription of OsRbohA, which encodes an Oryza sativa plasma membrane NADPH oxidase, were stimulated by drought”
Claim · model-written summary
OsRbohA was expressed in all examined tissues throughout development.
- Taxon
- riceOryza sativat0
- Gene
- OsRbohAe0
- Outcome
- expressed in all tissues examined throughout developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRbohA was expressed in all tissues examined throughout development”
t0“rice”
Claim · model-written summary
Heat, drought, salt, oxidative stress and methyl jasmonate upregulated OsRbohA mRNA.
- Taxon
- riceOryza sativat0
- Treatment
- heat, drought, salt, oxidative stress and methyl jasmonate treatmente0
- Gene
- OsRbohAe1
- Outcome
- mRNAe0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its mRNA was upregulated by a number of factors, including heat, drought, salt, oxidative stress and methyl jasmonate treatment”
t0“rice”
e1“OsRbohA was expressed in all tissues examined throughout development”
Claim · model-written summary
OsRbohA knockout upregulated other respiratory burst oxidase homolog genes.
- Taxon
- riceOryza sativat0
- Material
- osrbohAe0
- Treatment
- OsRbohA-knockoute0
- Gene
- OsRbohAe0
- Outcome
- expression of other respiratory burst oxidase homolog genese0
- Direction
- upregulatede0
- Comparator
- wild-type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type (WT), the OsRbohA-knockout mutant osrbohA exhibited upregulated expression of other respiratory burst oxidase homolog genes and multiple abnormal agronomic traits, including reduced biomass, low germination rate and decreased pollen viability and seed fertility”
t0“rice”
Claim · model-written summary
OsRbohA knockout reduced biomass compared with wild type.
- Taxon
- riceOryza sativat0
- Material
- osrbohAe0
- Treatment
- OsRbohA-knockoute0
- Outcome
- biomasse0
- Direction
- reducede0
- Comparator
- wild-type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type (WT), the OsRbohA-knockout mutant osrbohA exhibited upregulated expression of other respiratory burst oxidase homolog genes and multiple abnormal agronomic traits, including reduced biomass, low germination rate and decreased pollen viability and seed fertility”
t0“rice”
Claim · model-written summary
OsRbohA knockout lowered germination rate.
- Taxon
- riceOryza sativat0
- Treatment
- OsRbohA-knockoute0
- Outcome
- germination ratee0
- Direction
- lowe0
- Comparator
- wild-type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type (WT), the OsRbohA-knockout mutant osrbohA exhibited upregulated expression of other respiratory burst oxidase homolog genes and multiple abnormal agronomic traits, including reduced biomass, low germination rate and decreased pollen viability and seed fertility”
t0“rice”
Claim · model-written summary
OsRbohA knockout decreased pollen viability and seed fertility.
- Taxon
- riceOryza sativat0
- Treatment
- OsRbohA-knockoute0
- Outcome
- pollen viability and seed fertilitye0
- Direction
- decreasede0
- Comparator
- wild-type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with wild-type (WT), the OsRbohA-knockout mutant osrbohA exhibited upregulated expression of other respiratory burst oxidase homolog genes and multiple abnormal agronomic traits, including reduced biomass, low germination rate and decreased pollen viability and seed fertility”
t0“rice”
Claim · model-written summary
OsRbohA overexpression did not change these agronomic traits relative to wild type.
- Taxon
- riceOryza sativat0
- Treatment
- OsRbohA-overexpressinge0
- Outcome
- these traitse0
- Direction
- no differencese0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, OsRbohA-overexpressing transgenic plants showed no differences in these traits compared with WT”
t0“rice”
Claim · model-written summary
Knockout leaves and roots produced more ROS but contained less intracellular ROS than wild type.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although osrbohA leaves and roots produced more ROS than WT, the mutant had lesser intracellular ROS”
t0“rice”
Claim · model-written summary
Knockout plants had less intracellular ROS.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although osrbohA leaves and roots produced more ROS than WT, the mutant had lesser intracellular ROS”
t0“rice”
Claim · model-written summary
Overexpressing plants had higher intracellular and tissue ROS production.
- Taxon
- riceOryza sativat0
- Treatment
- OsRbohA-overexpressinge0
- Outcome
- ROS production at the intracellular level and in tissuese0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsRbohA-overexpressing transgenic plants exhibited higher ROS production at the intracellular level and in tissues”
t0“rice”
Claim · model-written summary
OsRbohA ablation impaired tolerance to water stresses.
- Taxon
- riceOryza sativat0
- Treatment
- Ablation of OsRbohAe0
- Gene
- OsRbohAe0
- Outcome
- tolerance of plants to various water stressese0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ablation of OsRbohA impaired the tolerance of plants to various water stresses”
t0“rice”
Claim · model-written summary
OsRbohA overexpression enhanced tolerance to water stresses.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas its overexpression enhanced the tolerance”
e1“Ablation of OsRbohA impaired the tolerance of plants to various water stresses”
t0“rice”
Claim · model-written summary
Knockout plants differentially expressed energy, transport, stress and transcriptional genes under normal growth.
- Taxon
- riceOryza sativat0
- Material
- osrbohAe0
- Outcome
- genes related to energy supply, substrate transport, stress response and transcriptional regulation were differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a number of genes related to energy supply, substrate transport, stress response and transcriptional regulation were differentially expressed in osrbohA plants even under normal growth conditions”
t0“rice”
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See all 80 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.