Abstract
The aim of this work was to evaluate the general role of the vacuolar pyrophosphatase proton pump (V-PPase) in sucrose accumulation in citrus species. First, three citrus V-PPase genes, designated CsVPP-1, CsVPP-2, and CsVPP-4, were identified in the citrus genome. CsVPP-1 and CsVPP-2 belonging to citrus type I V-PPase genes are targeted to the tonoplast, and CsVPP-4 belonging to citrus type II V-PPase genes is located in the Golgi bodies. Moreover, there was a significantly positive correlation between transcript levels of type I V-PPase genes and sucrose, rather than hexose, content in fruits of seven citrus cultivars. Drought and abscisic acid treatments significantly induced the CsVPP-1 and CsVPP-2 transcript levels, as well as the sucrose content. The overexpression of type I V-PPase genes significantly increased PPase activity, decreased pyrophosphate contents, and increased sucrose contents, whereas V-PPase inhibition produced the opposite effect in both citrus fruits and leaves. Furthermore, altering the expression levels of type I V-PPase genes significantly influenced the transcript levels of sucrose transporter genes. Taken together, this study demonstrated that CsVPP-1 and CsVPP-2 play key roles in sucrose storage in the vacuole by regulating pyrophosphate homeostasis, ultimately the sucrose biosynthesis and transcript levels of sucrose transport genes, providing a novel lead for engineering or breeding modified taste in citrus and other fruits.
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Claims 13
Claim · model-written summary
Three citrus V-PPase genes were identified.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three citrus V-PPase genes, designated CsVPP-1, CsVPP-2, and CsVPP-4, were identified in the citrus genome”
Claim · model-written summary
CsVPP-1 and CsVPP-2 were targeted to the tonoplast.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsVPP-1 and CsVPP-2 belonging to citrus type I V-PPase genes are targeted to the tonoplast”
Claim · model-written summary
CsVPP-4 was located in Golgi bodies.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsVPP-4 belonging to citrus type II V-PPase genes is located in the Golgi bodies”
Claim · model-written summary
Type I V-PPase transcript levels correlated positively with fruit sucrose, rather than hexose, content.
- Material
- seven citrus cultivarse0
- Organ / stage
- fruitse0
- Gene
- type I V-PPase genese0
- Outcome
- sucrose, rather than hexose, contente0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a significantly positive correlation between transcript levels of type I V-PPase genes and sucrose, rather than hexose, content in fruits of seven citrus cultivars”
Claim · model-written summary
Drought induced CsVPP-1 and CsVPP-2 transcript levels.
- Treatment
- Droughte0
- Gene
- CsVPP-1 and CsVPP-2e0
- Outcome
- CsVPP-1 and CsVPP-2 transcript levelse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought and abscisic acid treatments significantly induced the CsVPP-1 and CsVPP-2 transcript levels, as well as the sucrose content”
t0“citrus”
Claim · model-written summary
Abscisic acid induced CsVPP-1 and CsVPP-2 transcript levels.
- Treatment
- abscisic acide0
- Gene
- CsVPP-1 and CsVPP-2e0
- Outcome
- CsVPP-1 and CsVPP-2 transcript levelse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought and abscisic acid treatments significantly induced the CsVPP-1 and CsVPP-2 transcript levels, as well as the sucrose content”
t0“citrus”
Claim · model-written summary
Drought induced sucrose content.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought and abscisic acid treatments significantly induced the CsVPP-1 and CsVPP-2 transcript levels, as well as the sucrose content”
t0“citrus”
Claim · model-written summary
Abscisic acid induced sucrose content.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought and abscisic acid treatments significantly induced the CsVPP-1 and CsVPP-2 transcript levels, as well as the sucrose content”
t0“citrus”
Claim · model-written summary
Type I V-PPase overexpression increased PPase activity.
- Treatment
- overexpression of type I V-PPase genese0
- Gene
- type I V-PPase genese0
- Outcome
- PPase activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of type I V-PPase genes significantly increased PPase activity, decreased pyrophosphate contents, and increased sucrose contents”
t0“citrus”
Claim · model-written summary
Type I V-PPase overexpression decreased pyrophosphate contents.
- Treatment
- overexpression of type I V-PPase genese0
- Gene
- type I V-PPase genese0
- Outcome
- pyrophosphate contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of type I V-PPase genes significantly increased PPase activity, decreased pyrophosphate contents, and increased sucrose contents”
t0“citrus”
Claim · model-written summary
Type I V-PPase overexpression increased sucrose contents.
- Treatment
- overexpression of type I V-PPase genese0
- Gene
- type I V-PPase genese0
- Outcome
- sucrose contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of type I V-PPase genes significantly increased PPase activity, decreased pyrophosphate contents, and increased sucrose contents”
t0“citrus”
Claim · model-written summary
V-PPase inhibition decreased PPase activity and sucrose and increased pyrophosphate in fruit and leaves.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V-PPase inhibition produced the opposite effect in both citrus fruits and leaves”
Claim · model-written summary
Altering type I V-PPase expression influenced sucrose transporter gene transcripts.
- Treatment
- altering the expression levels of type I V-PPase genese0
- Gene
- type I V-PPase genese0
- Outcome
- transcript levels of sucrose transporter genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altering the expression levels of type I V-PPase genes significantly influenced the transcript levels of sucrose transporter genes”
t0“citrus”
The same questions in other papers
drought · quality: sugar starch · CitrusPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
drought · molecular: expression · CitrusPositive and negative results
6 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.