Abstract
The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos. The somatic embryos produced phenotypically normal plants devoid of any visual purple coloration. These results were also confirmed using protoplast transformation. There was minimal gene expression in unstressed one-year-old transgenic lines. Cold and drought stress did not have any effect on gene expression, while exogenous ABA and NaCl application resulted in a minor change in gene expression in several transgenic lines. When gas exchange was measured in intact leaves, the transgenic lines were similar to controls under the same environment. Our results provide conclusive evidence for the utilization of a plant-derived, embryo-specific visual reporter system for the genetic transformation of citrus. Such a system could aid in the development of an all-plant, consumer-friendly GM citrus tree.
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Claims 8
Claim · model-written summary
Transformation produced purple transgenic embryos separable from green nontransgenic embryos.
- Outcome
- purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryose0
- Value
- purplee0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green non-transgenic embryos.”
t0“citrus”
Claim · model-written summary
Transgenic somatic embryos yielded phenotypically normal plants without purple coloration.
- Outcome
- phenotypically normal plants devoid of any visual purple coloratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The somatic embryos produced phenotypically normal plants devoid of any visual purple coloration.”
t0“citrus”
Claim · model-written summary
Protoplast transformation confirmed the visual selection results.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results were also confirmed using protoplast transformation.”
t0“citrus”
Claim · model-written summary
Unstressed one year old transgenic lines showed minimal gene expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was minimal gene expression in unstressed one-year-old transgenic lines.”
t0“citrus”
Claim · model-written summary
Cold and drought did not affect gene expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold and drought stress did not have any effect on gene expression”
t0“citrus”
Claim · model-written summary
Exogenous ABA and NaCl caused a minor gene expression change in several transgenic lines.
- Material
- transgenic linese0
- Treatment
- exogenous ABA and NaCl applicatione0
- Outcome
- gene expressione0
- Direction
- minor changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous ABA and NaCl application resulted in a minor change in gene expression in several transgenic lines.”
t0“citrus”
Claim · model-written summary
Transgenic lines had gas exchange similar to controls under the same environment.
- Material
- transgenic linese0
- Organ / stage
- intact leavese0
- Outcome
- gas exchangee0
- Direction
- similare0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When gas exchange was measured in intact leaves, the transgenic lines were similar to controls under the same environment.”
t0“citrus”
Claim · model-written summary
Authors concluded the embryo specific visual reporter system can be used for citrus transformation.
- Outcome
- utilization of a plant-derived, embryo-specific visual reporter system for the genetic transformation of citruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“utilization of a plant-derived, embryo-specific visual reporter system for the genetic transformation of citrus.”
The same questions in other papers
cold · molecular: expression · CitrusPositive and negative results
1 in the opposite direction
- positive
drought · molecular: expression · CitrusPositive and negative results
3 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.