Abstract
We used rice dwarf1 (d1) mutants lacking a single-copy Galpha gene and addressed Galpha's role in disease resistance. d1 mutants exhibited a highly reduced hypersensitive response to infection by an avirulent race of rice blast. Activation of PR gene expression in the leaves of the mutants infected with rice blast was delayed for 24 h relative to the wild type. H(2)O(2) production and PR gene expression induced by sphingolipid elicitors (SE) were strongly suppressed in d1 cell cultures. Expression of the constitutively active OsRac1, a small GTPase Rac of rice, in d1 mutants restored SE-dependent defense signaling and resistance to rice blast. Galpha mRNA was induced by an avirulent race of rice blast and SE application on the leaf. These results indicated the role of Galpha in R gene-mediated disease resistance of rice. We have proposed a model for the defense signaling of rice in which the heterotrimeric G protein functions upstream of the small GTPase OsRac1 in the early steps of signaling.
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Claims 9
Claim · model-written summary
d1 mutants had markedly reduced hypersensitive response to avirulent rice blast.
- Taxon
- rice
Oryza sativat0 - Material
- d1 mutantse0
- Interactor
- rice blaste0pathogen
- Outcome
- hypersensitive responsee0
- Direction
- highly reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“d1 mutants exhibited a highly reduced hypersensitive response to infection by an avirulent race of rice blast.”
t0“rice”
Claim · model-written summary
PR gene activation in infected mutant leaves was delayed 24 hours versus wild type.
- Taxon
- riceOryza sativat0
- Interactor
- rice blaste0pathogen
- Gene
- PR genee0
- Outcome
- Activation of PR gene expressione0
- Direction
- delayede0
- Value
- 24 he0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activation of PR gene expression in the leaves of the mutants infected with rice blast was delayed for 24 h relative to the wild type.”
t0“rice”
Claim · model-written summary
Elicitor-induced H2O2 production was strongly suppressed in d1 cell cultures.
- Taxon
- riceOryza sativat0
- Material
- d1 cell culturese0
- Treatment
- sphingolipid elicitors (SE)e0
- Outcome
- H(2)O(2) productione0
- Direction
- strongly suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(2)O(2) production and PR gene expression induced by sphingolipid elicitors (SE) were strongly suppressed in d1 cell cultures.”
t0“rice”
Claim · model-written summary
Elicitor-induced PR expression was strongly suppressed in d1 cultures.
- Taxon
- riceOryza sativat0
- Material
- d1 cell culturese0
- Treatment
- sphingolipid elicitors (SE)e0
- Gene
- PR genee0
- Outcome
- PR gene expressione0
- Direction
- strongly suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(2)O(2) production and PR gene expression induced by sphingolipid elicitors (SE) were strongly suppressed in d1 cell cultures.”
t0“rice”
Claim · model-written summary
Active OsRac1 expression restored elicitor-dependent defense signaling in d1 rice.
- Taxon
- riceOryza sativae0
- Material
- d1 mutantse0
- Treatment
- Expression of the constitutively active OsRac1e0
- Gene
- OsRac1e0
- Outcome
- SE-dependent defense signalinge0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the constitutively active OsRac1, a small GTPase Rac of rice, in d1 mutants restored SE-dependent defense signaling and resistance to rice blast.”
Claim · model-written summary
Active OsRac1 expression restored rice blast resistance in d1 mutants.
- Taxon
- riceOryza sativae0
- Material
- d1 mutantse0
- Treatment
- Expression of the constitutively active OsRac1e0
- Interactor
- rice blaste0pathogen
- Gene
- OsRac1e0
- Outcome
- resistance to rice blaste0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the constitutively active OsRac1, a small GTPase Rac of rice, in d1 mutants restored SE-dependent defense signaling and resistance to rice blast.”
Claim · model-written summary
Avirulent rice blast induced Galpha mRNA.
- Taxon
- riceOryza sativat0
- Interactor
- rice blaste0pathogen
- Gene
- Galphae0
- Outcome
- Galpha mRNAe0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Galpha mRNA was induced by an avirulent race of rice blast and SE application on the leaf.”
t0“rice”
Claim · model-written summary
Sphingolipid elicitor application induced Galpha mRNA.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Galpha mRNA was induced by an avirulent race of rice blast and SE application on the leaf.”
t0“rice”
Claim · model-written summary
The proposed rice defense model places Galpha upstream of OsRac1.
- Taxon
- riceOryza sativat0
- Gene
- OsRac1e0
- Outcome
- early steps of signalinge0
- Qualifier
- heterotrimeric G protein functions upstreame0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heterotrimeric G protein functions upstream of the small GTPase OsRac1 in the early steps of signaling.”
t0“rice”
The same questions in other papers
transgenic · disease · Oryza sativa (rice)Positive and negative results
31 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- negative
- negative
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.