Abstract
Glyphosate-resistant crops developed by the CP4-EPSPS gene from Agrobacterium have been planted on a massive scale globally, which benefits from the high efficiency and broad spectrum of glyphosate in weed control. Some glyphosate-resistant (GR) genes from microbes have been reported, which might raise biosafety concerns. Most of them were obtained through a hygromycin- HPT transformation system. Here we reported the plant source with 5-enolpyruvylshikimate-3-phosphate synthase ( EPSPS ) gene from goosegrass endowed rice with high resistance to glyphosate. The integrations and inheritability of the transgenes in the rice genome were investigated within two generations. The EiEPSPS transgenic plants displayed similar growth and development to wild type under no glyphosate selection pressure but better reproductive performance under lower glyphosate selection pressure. Furthermore, we reconstructed a binary vector pCEiEPSPS and established the whole stage glyphosate selection using the vector. The Glyphosate-pCEiEPSPS selection system showed a significantly higher transformation efficiency compared with the hygromycin- HPT transformation system. Our results provided a promising alternative gene resource to the development of GR plants and also extended the plant transformation toolbox.
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Claims 4
Claim · model-written summary
Goosegrass EPSPS gave rice high glyphosate resistance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plant source with 5-enolpyruvylshikimate-3-phosphate synthase ( EPSPS ) gene from goosegrass endowed rice with high resistance to glyphosate.”
Claim · model-written summary
EiEPSPS transgenic rice resembled wild type in growth and development without glyphosate selection.
- Taxon
- Rice
Oryza sativat0 - Treatment
- EiEPSPSe0
- Outcome
- growth and developmente0
- Direction
- similare0
- Qualifier
- no glyphosate selection pressuree0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The EiEPSPS transgenic plants displayed similar growth and development to wild type under no glyphosate selection pressure”
t0“Rice”
Claim · model-written summary
EiEPSPS transgenic rice had better reproductive performance under lower glyphosate pressure.
- Taxon
- RiceOryza sativat0
- Treatment
- lower glyphosate selection pressuree0
- Outcome
- reproductive performancee0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but better reproductive performance under lower glyphosate selection pressure.”
t0“Rice”
Claim · model-written summary
The glyphosate-pCEiEPSPS selection system transformed rice more efficiently than the hygromycin-HPT system.
- Taxon
- RiceOryza sativat0
- Treatment
- Glyphosate-pCEiEPSPS selection systeme0
- Outcome
- transformation efficiencye0
- Direction
- highere0
- Comparator
- hygromycin- HPT transformation systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Glyphosate-pCEiEPSPS selection system showed a significantly higher transformation efficiency compared with the hygromycin- HPT transformation system.”
t0“Rice”
The same questions in other papers
5-O-(1-carboxylatovinyl)-3-phosphonatoshikimate · disease · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.