Abstract
SA (Salicylic acid) and JA (Jasmonic acid)/ET (Ethylene) are the defense and growth hormone regulators involved in alleviating the biotic and abiotic stresses. WRKY and NAC genes are contributors to plant resistance due to their active role in SA and JA/ET defense mechanism. WRKY and NAC transcription factors (ptHR293 and ptHR759, respectively) were serially selected (by performing cDNA library functional screening, homology analysis, antioxidant enzymes, ROS burst, callose deposition and qRT-PCR analysis) from Pinellia ternata and transformed into Nicotiana benthamiana. Hybrids were generated to analyze the stability of disease resistance. UPLC-QTOF-MS was performed to study the bioactive compounds. Study demonstrated that ptHR293 and ptHR759 had potential to trigger ROS burst and callose deposition in N. benthamiana together with the activation of PR-genes and antioxidant enzymes. In transformed N. benthamiana, relative expression of PR-1a (Pathogenesis related-1a) and PDF1.2 (Plant defensin 1.2) was upregulated upto 21 folds and 4 folds for ptHR293 transformed N. benthamiana. While PR-1a and PDF1.2 exhibited 8 folds and 26 folds upregulation for ptHR759 transformed N. benthamiana. ptHR293 + ptHR759-F1 hybrids also exhibited a significant level of PR-gene expression. Significantly high resistance against Botrytis cinerea without influencing the standard seed germination, root and shoot length of transformed N. benthamiana was also observed. A significant induction of bioactive compounds was also observed in ptHR293 transformed N. benthamiana. Conclusively, heterologous transcription factors, ptHR293 and ptHR759, perform their specific role in the activation of SA and JA/ET mediated defense mechanism.
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Claims 12
Claim · model-written summary
ptHR293 induced ROS burst and callose deposition in N. benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Treatment
- ptHR293e0
- Gene
- ptHR293e0
- Outcome
- ROS burst and callose depositione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ptHR293 and ptHR759 had potential to trigger ROS burst and callose deposition in N. benthamiana”
Claim · model-written summary
ptHR759 induced ROS burst and callose deposition in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- ptHR759e0
- Gene
- ptHR759e0
- Outcome
- ROS burst and callose depositione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ptHR293 and ptHR759 had potential to trigger ROS burst and callose deposition in N. benthamiana”
Claim · model-written summary
ptHR293-transformed N. benthamiana had PR-1a expression upregulated up to 21-fold.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- ptHR293 transformed N. benthamianae0
- Gene
- PR-1a (Pathogenesis related-1a)e0
- Outcome
- relative expression of PR-1ae0
- Direction
- upregulatede0
- Value
- 21 foldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative expression of PR-1a (Pathogenesis related-1a) and PDF1.2 (Plant defensin 1.2) was upregulated upto 21 folds and 4 folds for ptHR293 transformed N. benthamiana”
Claim · model-written summary
ptHR293-transformed N. benthamiana had PDF1.2 expression upregulated fourfold.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- ptHR293 transformed N. benthamianae0
- Gene
- PDF1.2 (Plant defensin 1.2)e0
- Outcome
- PDF1.2 (Plant defensin 1.2)e0
- Direction
- upregulatede0
- Value
- 4 foldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“relative expression of PR-1a (Pathogenesis related-1a) and PDF1.2 (Plant defensin 1.2) was upregulated upto 21 folds and 4 folds for ptHR293 transformed N. benthamiana”
Claim · model-written summary
ptHR759-transformed N. benthamiana had PR-1a expression upregulated eightfold.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- ptHR759 transformed N. benthamianae0
- Gene
- PR-1ae0
- Outcome
- PR-1ae0
- Direction
- upregulatione0
- Value
- 8 foldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PR-1a and PDF1.2 exhibited 8 folds and 26 folds upregulation for ptHR759 transformed N. benthamiana”
Claim · model-written summary
ptHR759-transformed N. benthamiana had PDF1.2 expression upregulated 26-fold.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- ptHR759 transformed N. benthamianae0
- Gene
- PDF1.2e0
- Outcome
- PDF1.2e0
- Direction
- upregulatione0
- Value
- 26 foldse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PR-1a and PDF1.2 exhibited 8 folds and 26 folds upregulation for ptHR759 transformed N. benthamiana”
Claim · model-written summary
ptHR293 + ptHR759-F1 hybrids showed significant PR-gene expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Material
- ptHR293 + ptHR759-F1 hybridse0
- Gene
- PR-genee0
- Outcome
- PR-gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ptHR293 + ptHR759-F1 hybrids also exhibited a significant level of PR-gene expression”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transformed N. benthamiana showed significantly high resistance to Botrytis cinerea.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transformed N. benthamianae0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- resistance against Botrytis cinereae0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significantly high resistance against Botrytis cinerea without influencing the standard seed germination, root and shoot length of transformed N. benthamiana”
Claim · model-written summary
Transformation did not influence seed germination of N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transformed N. benthamianae0
- Outcome
- seed germinatione0
- Direction
- without influencinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“without influencing the standard seed germination, root and shoot length of transformed N. benthamiana”
Claim · model-written summary
Transformation did not influence root and shoot length of N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transformed N. benthamianae0
- Outcome
- root and shoot lengthe0
- Direction
- without influencinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“without influencing the standard seed germination, root and shoot length of transformed N. benthamiana”
Claim · model-written summary
ptHR293 transformation significantly induced bioactive compounds in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- ptHR293 transformed N. benthamianae0
- Outcome
- bioactive compoundse0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significant induction of bioactive compounds was also observed in ptHR293 transformed N. benthamiana”
Claim · model-written summary
The authors conclude that ptHR293 and ptHR759 activate SA- and JA/ET-mediated defense mechanisms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- ptHR293 and ptHR759e0
- Outcome
- activation of SA and JA/ET mediated defense mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous transcription factors, ptHR293 and ptHR759, perform their specific role in the activation of SA and JA/ET mediated defense mechanism”
t0“Nicotiana benthamiana”
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