Abstract
Necrotrophic pathogens such as Botrytis cinerea cause significant crop yield losses. Plant CCCH proteins play important roles in pathogen resistance responses. However, the CCCH-mediated defense mechanisms against necrotrophic pathogens are unclear. Here, we report that the Arabidopsis CCCH protein C3H14 positively regulates basal defense against B. cinerea mainly by WRKY33 signaling. Simultaneous mutation of C3H14 and its paralog C3H15 resulted in enhanced susceptibility to B. cinerea, while C3H14 or C3H15 overexpression lines exhibited reduced susceptibility. A large number of differentially expressed genes (DEGs) were present in the c3h14c3h15 double mutant and C3H14 overexpression plants compared with wild-type plants at 24 hr post infection. These DEGs covered over one third of B. cinerea-responsive WRKY33 targets, including genes involved in jasmonic acid (JA)/ethylene (ET) signaling, and camalexin biosynthesis. Genetic analysis indicated that C3H14 mainly depended on WRKY33 to modulate defense against B. cinerea. Moreover, C3H14 activated the WRKY33-ORA59 and -PAD3 cascades to correspondingly control JA/ET- and camalexin-mediated defense responses. However, C3H14 was essential for B. cinerea-induced production of 12-oxo-phytodienoic acid and it also directly mediated ORA59-dependent JA/ET signaling after infection. Therefore, C3H14 may act as a novel transcriptional regulator of the WRKY33-mediated defense pathway.
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Claims 9
Claim · model-written summary
C3H14 positively regulates basal defense against B. cinerea, mainly through WRKY33 signaling.
- Taxon
- Arabidopsis
Arabidopsise0 - Interactor
- B. cinerea
Botrytis cinereae0pathogen - Gene
- C3H14e0
- Outcome
- basal defense against B. cinereae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Arabidopsis CCCH protein C3H14 positively regulates basal defense against B. cinerea mainly by WRKY33 signaling”
Claim · model-written summary
Simultaneous mutation of C3H14 and C3H15 increased susceptibility to B. cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Simultaneous mutation of C3H14 and its paralog C3H15e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- C3H14 and its paralog C3H15e0
- Outcome
- susceptibility to B. cinereae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Simultaneous mutation of C3H14 and its paralog C3H15 resulted in enhanced susceptibility to B. cinerea”
t0“ArabidopsisCCCH”
Claim · model-written summary
C3H14 or C3H15 overexpression reduced susceptibility.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- C3H14 or C3H15 overexpressione0
- Gene
- C3H14 or C3H15e0
- Outcome
- susceptibilitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C3H14 or C3H15 overexpression lines exhibited reduced susceptibility”
t0“ArabidopsisCCCH”
Claim · model-written summary
Many genes were differentially expressed in the double mutant and C3H14 overexpression plants relative to wild type at 24 hr after infection.
- Taxon
- ArabidopsisArabidopsist0
- Material
- c3h14c3h15 double mutant and C3H14 overexpression plantse0
- Outcome
- differentially expressed genes (DEGs)e0
- Qualifier
- 24 hr post infectione0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A large number of differentially expressed genes (DEGs) were present in the c3h14c3h15 double mutant and C3H14 overexpression plants compared with wild-type plants at 24 hr post infection”
t0“ArabidopsisCCCH”
Claim · model-written summary
The differentially expressed genes covered over one third of B. cinerea responsive WRKY33 targets.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- WRKY33e0
- Outcome
- B. cinerea-responsive WRKY33 targetse0
- Value
- over one thirde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These DEGs covered over one third of B. cinerea-responsive WRKY33 targets”
t0“ArabidopsisCCCH”
Claim · model-written summary
C3H14 mainly depended on WRKY33 to modulate defense against B. cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- C3H14e0
- Outcome
- defense against B. cinereae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C3H14 mainly depended on WRKY33 to modulate defense against B. cinerea”
t0“ArabidopsisCCCH”
Claim · model-written summary
C3H14 activated the WRKY33-ORA59 and -PAD3 cascades controlling JA/ET and camalexin defense responses.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- C3H14e0
- Compound
- camalexine0
- Outcome
- JA/ET- and camalexin-mediated defense responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C3H14 activated the WRKY33-ORA59 and -PAD3 cascades to correspondingly control JA/ET- and camalexin-mediated defense responses”
t0“ArabidopsisCCCH”
Claim · model-written summary
C3H14 was essential for B. cinerea induced production of 12-oxo-phytodienoic acid.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- C3H14e0
- Outcome
- production of 12-oxo-phytodienoic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C3H14 was essential for B. cinerea-induced production of 12-oxo-phytodienoic acid”
t0“ArabidopsisCCCH”
Claim · model-written summary
C3H14 directly mediated ORA59 dependent JA/ET signaling after infection.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- C3H14t0
- Outcome
- ORA59-dependent JA/ET signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it also directly mediated ORA59-dependent JA/ET signaling after infection”
t0“ArabidopsisCCCH protein C3H14”
The same questions in other papers
loss of function · Botrytis cinerea · ArabidopsisPositive and negative results
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.