Abstract
Botrytis cinerea is a necrotrophic fungus that infects more than 200 plant species, causing significant economic losses worldwide. Due to its diverse pathogenicity, extensive efforts have been made to unravel its pathogenic mechanisms. However, the function of the effectors underlying B. cinerea virulence remains largely unknown. In this study, we identified an effector BcSCR1 (B. cinerea small cysteine-rich protein 1) and found that it interacts with the transcription factor PFB1 (Pericycle Factor Type-B1) in Arabidopsis thaliana. Ectopic expression of BcSCR1 in plants resulted in decreased resistance to B. cinerea infection, while disruption of BcSCR1 in B. cinerea significantly decreased its aggressiveness toward host plants. In addition, the mutation of PFB1 in Arabidopsis increased resistance of plants to B. cinerea infection. PFB1 directly activated the expression of OPR3 and WRKY33 under normal conditions, while the binding of BcSCR1 to PFB1 repressed its transcriptional activation activity during B. cinerea infection. These findings reveal that B. cinerea employs the effector protein BcSCR1 to promote infection by suppressing the plant's immune defences.
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Claims 6
Claim · model-written summary
Botrytis effector BcSCR1 interacts with Arabidopsis PFB1.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Interactor
- B. cinerea
Botrytis cinereae0pathogen - Gene
- PFB1e0
- Outcome
- interacts with the transcription factor PFB1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this study, we identified an effector BcSCR1 (B. cinerea small cysteine-rich protein 1) and found that it interacts with the transcription factor PFB1 (Pericycle Factor Type-B1) in Arabidopsis thaliana.”
Claim · model-written summary
Ectopic BcSCR1 expression decreased plant resistance to Botrytis.
- Taxon
- plantsViridiplantaee0
- Treatment
- Ectopic expression of BcSCR1e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- resistance to B. cinerea infectione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of BcSCR1 in plants resulted in decreased resistance to B. cinerea infection, while disruption of BcSCR1 in B. cinerea significantly decreased its aggressiveness toward host plants.”
Claim · model-written summary
Disrupting BcSCR1 significantly decreased Botrytis aggressiveness toward host plants.
- Taxon
- host plantse0
- Treatment
- disruption of BcSCR1 in B. cinereae0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- aggressiveness toward host plantse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of BcSCR1 in plants resulted in decreased resistance to B. cinerea infection, while disruption of BcSCR1 in B. cinerea significantly decreased its aggressiveness toward host plants.”
Claim · model-written summary
Arabidopsis PFB1 mutation increased resistance to Botrytis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mutation of PFB1e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- PFB1e0
- Outcome
- resistance of plants to B. cinerea infectione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, the mutation of PFB1 in Arabidopsis increased resistance of plants to B. cinerea infection.”
Claim · model-written summary
PFB1 directly activated OPR3 and WRKY33 expression under normal conditions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- PFB1e0
- Outcome
- expression of OPR3 and WRKY33e0
- Direction
- activatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PFB1 directly activated the expression of OPR3 and WRKY33 under normal conditions, while the binding of BcSCR1 to PFB1 repressed its transcriptional activation activity during B. cinerea infection.”
e1“Arabidopsis thaliana”
Claim · model-written summary
BcSCR1 binding to PFB1 repressed its transcriptional activation during infection.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- PFB1e0
- Outcome
- transcriptional activation activitye0
- Direction
- repressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PFB1 directly activated the expression of OPR3 and WRKY33 under normal conditions, while the binding of BcSCR1 to PFB1 repressed its transcriptional activation activity during B. cinerea infection.”
e1“Arabidopsis thaliana”
The same questions in other papers
loss of function · Botrytis cinerea · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 16 in the opposite direction
- negative
- negative
- negative
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.