Abstract
Plant WRKY transcription factors are key regulatory components of plant responses to microbial infection. In addition to regulating the expression of defense-related genes, WRKY transcription factors have also been shown to regulate cross-talk between jasmonate- and salicylate-regulated disease response pathways. The two pathways mediate resistance against different types of microbial pathogens, and there are numerous reports of antagonistic interactions between them. Here we show that mutations of the Arabidopsis WRKY33 gene encoding a WRKY transcription factor cause enhanced susceptibility to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola concomitant with reduced expression of the jasmonate-regulated plant defensin PDF1.2 gene. Ectopic over-expression of WRKY33, on the other hand, increases resistance to the two necrotrophic fungal pathogens. The wrky33 mutants do not show altered responses to a virulent strain of the bacterial pathogen Pseudomonas syringae, although the ectopic expression of WRKY33 results in enhanced susceptibility to this pathogen. The susceptibility of WRKY33-over-expressing plants to P. syringae is associated with reduced expression of the salicylate-regulated PR-1 gene. The WRKY33 transcript is induced in response to pathogen infection, or treatment with salicylate or the paraquat herbicide that generates activated oxygen species in exposed cells. WRKY33 is localized to the nucleus of plant cells and recognizes DNA molecules containing the TTGACC W-box sequence. Together, these results indicate that pathogen-induced WRKY33 is an important transcription factor that regulates the antagonistic relationship between defense pathways mediating responses to P. syringae and necrotrophic pathogens.
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Claims 9
Claim · model-written summary
Mutations of the Arabidopsis WRKY33 gene cause enhanced susceptibility to Botrytis cinerea and Alternaria brassicicola
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- mutations of the Arabidopsis WRKY33 genee0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Second interactor
- Alternaria brassicicola
Alternaria brassicicolae0pathogen - Gene
- WRKY33e0
- Outcome
- susceptibilitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e0“mutations of the Arabidopsis WRKY33 gene encoding a WRKY transcription factor cause enhanced susceptibility to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola”
Claim · model-written summary
Mutations of the Arabidopsis WRKY33 gene reduce expression of the jasmonate-regulated defensin PDF1.2 gene
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- mutations of the Arabidopsis WRKY33 genee0
- Gene
- PDF1.2e1
- Outcome
- expression of the jasmonate-regulated plant defensin PDF1.2 genee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e0“mutations of the Arabidopsis WRKY33 gene encoding a WRKY transcription factor cause enhanced susceptibility to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola”
e1“concomitant with reduced expression of the jasmonate-regulated plant defensin PDF1.2 gene”
Claim · model-written summary
Ectopic over-expression of WRKY33 increases resistance to two necrotrophic fungal pathogens
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Ectopic over-expression of WRKY33e2
- Interactor
- necrotrophic fungal pathogense2pathogen
- Outcome
- resistance to the two necrotrophic fungal pathogense2
- Direction
- increasese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e2“Ectopic over-expression of WRKY33, on the other hand, increases resistance to the two necrotrophic fungal pathogens”
Claim · model-written summary
The wrky33 mutants show no altered responses to a virulent strain of Pseudomonas syringae
- Taxon
- ArabidopsisArabidopsist0
- Material
- wrky33 mutantse3
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee3pathogen - Outcome
- responsese3
- Qualifier
- virulent straine3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e3“The wrky33 mutants do not show altered responses to a virulent strain of the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
Ectopic expression of WRKY33 enhances susceptibility to Pseudomonas syringae
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopic expression of WRKY33e4
- Interactor
- Pseudomonas syringaePseudomonas syringaee3pathogen
- Outcome
- susceptibilitye4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e4“the ectopic expression of WRKY33 results in enhanced susceptibility to this pathogen”
e3“The wrky33 mutants do not show altered responses to a virulent strain of the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
Susceptibility of WRKY33-over-expressing plants to P. syringae is associated with reduced expression of the PR-1 gene
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- WRKY33-over-expressing plantse5
- Interactor
- P. syringaePseudomonas syringaee5pathogen
- Gene
- PR-1e5
- Outcome
- expressione5
- Direction
- reducede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e5“The susceptibility of WRKY33-over-expressing plants to P. syringae is associated with reduced expression of the salicylate-regulated PR-1 gene”
Claim · model-written summary
The WRKY33 transcript is induced by pathogen infection and by salicylate or paraquat treatment
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- pathogen infectione6
- Gene
- WRKY33e6
- Outcome
- WRKY33 transcripte6
- Direction
- inducede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e6“The WRKY33 transcript is induced in response to pathogen infection, or treatment with salicylate or the paraquat herbicide”
Claim · model-written summary
WRKY33 is localized to the nucleus of Arabidopsis plant cells
- Taxon
- ArabidopsisArabidopsist0
- Gene
- WRKY33e7
- Outcome
- localized to the nucleuse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e7“WRKY33 is localized to the nucleus of plant cells and recognizes DNA molecules containing the TTGACC W-box sequence”
Claim · model-written summary
WRKY33 recognizes DNA molecules containing the TTGACC W-box sequence
- Taxon
- ArabidopsisArabidopsist0
- Gene
- WRKY33e7
- Outcome
- recognizes DNA molecules containing the TTGACC W-box sequencee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens”
e7“WRKY33 is localized to the nucleus of plant cells and recognizes DNA molecules containing the TTGACC W-box sequence”
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See all 197 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.