Abstract
Genes and proteins related to patatin, the major storage protein of potato tubers, have been identified in many plant species and shown to be induced by a variety of environmental stresses. The Arabidopsis patatin-like gene family (PLPs) comprises nine members, two of which (PLP2 and PLP7) are strongly induced in leaves challenged with fungal and bacterial pathogens. Here we show that accumulation of PLP2 protein in response to Botrytis cinerea or Pseudomonas syringae pv. tomato (avrRpt2) is dependent on jasmonic acid and ethylene signaling, but is not dependent on salicylic acid. Expression of a PLP2-green fluorescent protein (GFP) fusion protein and analysis of recombinant PLP2 indicates that PLP2 encodes a cytoplasmic lipid acyl hydrolase with wide substrate specificity. Transgenic plants with altered levels of PLP2 protein were generated and assayed for pathogen resistance. Plants silenced for PLP2 expression displayed enhanced resistance to B. cinerea, whereas plants overexpressing PLP2 were much more sensitive to this necrotrophic fungus. We also established a positive correlation between the level of PLP2 expression in transgenic plants and cell death or damage in response to paraquat treatment or infection by avirulent P. syringae. Interestingly, repression of PLP2 expression increased resistance to avirulent bacteria, while PLP2-overexpressing plants multiplied avirulent bacteria close to the titers reached by virulent bacteria. Collectively, the data indicate that PLP2-encoded lipolytic activity can be exploited by pathogens with different lifestyles to facilitate host colonization. In particular PLP2 potentiates plant cell death inflicted by Botrytis and reduces the efficiency of the hypersensitive response in restricting the multiplication of avirulent bacteria. Both effects are possibly mediated by providing fatty acid precursors of bioactive oxylipins.
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Claims 16
Claim · model-written summary
PLP2 and PLP7 are strongly induced in leaves challenged with fungal and bacterial pathogens.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- leavese0
- Gene
- PLP2 and PLP7e0
- Outcome
- strongly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis patatin-like gene family (PLPs) comprises nine members, two of which (PLP2 and PLP7) are strongly induced in leaves challenged with fungal and bacterial pathogens.”
Claim · model-written summary
Arabidopsis has nine PLP family members.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- PLPse0
- Outcome
- patatin-like gene familye0
- Value
- nine memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis patatin-like gene family (PLPs) comprises nine members”
Claim · model-written summary
PLP2 accumulation after Botrytis cinerea challenge depends on jasmonic acid and ethylene signaling.
- Taxon
- ArabidopsisArabidopsise1
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Gene
- PLP2e0
- Outcome
- accumulation of PLP2 proteine0
- Qualifier
- jasmonic acid and ethylene signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation of PLP2 protein in response to Botrytis cinerea or Pseudomonas syringae pv. tomato (avrRpt2) is dependent on jasmonic acid and ethylene signaling”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 accumulation after Pseudomonas syringae challenge depends on jasmonic acid and ethylene signaling.
- Taxon
- ArabidopsisArabidopsise1
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe0pathogen - Gene
- PLP2e0
- Outcome
- accumulation of PLP2 proteine0
- Qualifier
- jasmonic acid and ethylene signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation of PLP2 protein in response to Botrytis cinerea or Pseudomonas syringae pv. tomato (avrRpt2) is dependent on jasmonic acid and ethylene signaling”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 accumulation after pathogen challenge does not depend on salicylic acid.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PLP2e0
- Outcome
- accumulation of PLP2 proteine0
- Qualifier
- not dependent on salicylic acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation of PLP2 protein in response to Botrytis cinerea or Pseudomonas syringae pv. tomato (avrRpt2) is dependent on jasmonic acid and ethylene signaling, but is not dependent on salicylic acid”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 encodes a cytoplasmic lipid acyl hydrolase with wide substrate specificity.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PLP2e0
- Outcome
- cytoplasmic lipid acyl hydrolase with wide substrate specificitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLP2 encodes a cytoplasmic lipid acyl hydrolase with wide substrate specificity”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
Silencing PLP2 enhanced resistance to B. cinerea.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- silenced for PLP2 expressione0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- PLP2e0
- Outcome
- resistance to B. cinereae0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants silenced for PLP2 expression displayed enhanced resistance to B. cinerea”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
Overexpressing PLP2 made plants much more sensitive to B. cinerea.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- overexpressing PLP2e0
- Interactor
- B. cinereaBotrytis cinereae1pathogen
- Gene
- PLP2e0
- Outcome
- sensitive to this necrotrophic funguse0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants overexpressing PLP2 were much more sensitive to this necrotrophic fungus”
e1“Plants silenced for PLP2 expression displayed enhanced resistance to B. cinerea”
e2“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 expression correlated positively with cell death or damage after paraquat treatment.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- paraquat treatmente0
- Gene
- PLP2e0
- Outcome
- cell death or damagee0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a positive correlation between the level of PLP2 expression in transgenic plants and cell death or damage in response to paraquat treatment”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 expression correlated positively with cell death or damage after avirulent P. syringae infection.
- Taxon
- ArabidopsisArabidopsise1
- Interactor
- P. syringaePseudomonas syringaee0pathogen
- Gene
- PLP2e0
- Outcome
- cell death or damagee0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a positive correlation between the level of PLP2 expression in transgenic plants and cell death or damage in response to paraquat treatment or infection by avirulent P. syringae”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
Repressing PLP2 increased resistance to avirulent bacteria.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- repression of PLP2 expressione0
- Interactor
- avirulent bacteriae0pathogen
- Gene
- PLP2e0
- Outcome
- resistance to avirulent bacteriae0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“repression of PLP2 expression increased resistance to avirulent bacteria”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 overexpression allowed avirulent bacteria to multiply close to virulent bacterial titers.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- PLP2-overexpressinge0
- Interactor
- avirulent bacteriae0pathogen
- Gene
- PLP2e0
- Outcome
- multiplied avirulent bacteriae0
- Comparator
- virulent bacteriae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLP2-overexpressing plants multiplied avirulent bacteria close to the titers reached by virulent bacteria”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 lipolytic activity may facilitate pathogen colonization.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PLP2e0
- Outcome
- host colonizatione0
- Qualifier
- lipolytic activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLP2-encoded lipolytic activity can be exploited by pathogens with different lifestyles to facilitate host colonization”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 potentiates plant cell death inflicted by Botrytis.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- PLP2e0
- Outcome
- plant cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLP2 potentiates plant cell death inflicted by Botrytis”
e1“The Arabidopsis patatin-like gene family”
Claim · model-written summary
PLP2 reduces hypersensitive response restriction of avirulent bacterial multiplication.
- Taxon
- ArabidopsisArabidopsise2
- Interactor
- avirulent bacteriae0pathogen
- Gene
- PLP2e1
- Outcome
- efficiency of the hypersensitive responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduces the efficiency of the hypersensitive response in restricting the multiplication of avirulent bacteria”
e1“PLP2 potentiates plant cell death”
e2“The Arabidopsis patatin-like gene family”
Claim · model-written summary
The effects of PLP2 may be mediated by fatty acid precursors of bioactive oxylipins.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- PLP2e1
- Compound
- bioactive oxylipinse0
- Outcome
- Both effectse0
- Qualifier
- possibly mediatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both effects are possibly mediated by providing fatty acid precursors of bioactive oxylipins”
e1“PLP2 potentiates plant cell death”
e2“The Arabidopsis patatin-like gene family”
The same questions in other papers
loss of function · Botrytis cinerea · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 16 in the opposite direction
- negative
- negative
- negative
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.