Abstract
Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in a very wide range of plant species. Subtilisin-like proteases (or subtilases) are a very diverse family of serine proteases present in many organisms and are reported to have a broad spectrum of biological functions. Here, we identified two genes encoding subtilisin-like proteases (Bcser1 and Bcser2) in the genome of B. cinerea, both of which contain an inhibitor I9 domain and a peptidase S8 domain. The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2. Interestingly, deletion of Bcser1 had no effect on the fungal development or virulence of B. cinerea. However, deletion of Bcser2 or double deletion of Bcser1 and Bcser2 severely impaired the hyphal growth, sclerotial formation and conidiation of B. cinerea. We also found that ∆Bcser2 and ∆Bcser1/2 could not form complete infection cushions and then lost the ability to infect intact plant leaves of Arabidopsis and tomato but could infect wounded plant tissues. Taken together, our results indicate that the subtilisin-like protease Bcser2 is crucial for the sclerotial formation, conidiation, and virulence of B. cinerea.
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Claims 5
Claim · model-written summary
Deletion of Bcser2 severely impaired hyphal growth, sclerotial formation and conidiation of Botrytis cinerea
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae2 - Treatment
- deletion of Bcser2e3
- Interactor
- Botrytis cinerea
Botrytis cinereae1pathogen - Gene
- Bcser2e3
- Outcome
- hyphal growth, sclerotial formation and conidiatione3
- Direction
- severely impairede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in a very wide range of plant species”
e2“The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2”
e3“deletion of Bcser2 or double deletion of Bcser1 and Bcser2 severely impaired the hyphal growth, sclerotial formation and conidiation of B. cinerea”
Claim · model-written summary
Deletion of Bcser1 had no effect on fungal development or virulence of Botrytis cinerea
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Treatment
- deletion of Bcser1e5
- Interactor
- B. cinereaBotrytis cinereae5pathogen
- Gene
- Bcser1e5
- Outcome
- fungal development or virulencee5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2”
e5“deletion of Bcser1 had no effect on the fungal development or virulence of B. cinerea”
Claim · model-written summary
Bcser1 and Bcser2 expression increased during the sclerotial forming stage and later hyphal infection on Arabidopsis thaliana leaves
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Organ / stage
- sclerotial forming stagee2
- Gene
- Bcser1 and Bcser2e2
- Outcome
- expression levelse2
- Direction
- increasede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2”
Claim · model-written summary
Bcser1 up-regulation was significantly higher than Bcser2 up-regulation during Botrytis cinerea infection of Arabidopsis thaliana leaves
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Gene
- Bcser1e2
- Outcome
- up-regulation of Bcser1e2
- Direction
- significantly highere2
- Comparator
- Bcser2e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The expression levels of Bcser1 and Bcser2 increased during the sclerotial forming stage, as well as during a later stage of hyphal infection on Arabidopsis thaliana leaves, but the up-regulation of Bcser1 was significantly higher than that of Bcser2”
Claim · model-written summary
Deletion of Bcser2 or of both Bcser1 and Bcser2 prevented infection of intact Arabidopsis and tomato leaves by Botrytis cinerea
- Taxon
- Arabidopsis and tomatoArabidopsis
Solanum lycopersicume4 - Treatment
- deletion of Bcser2 or double deletion of Bcser1 and Bcser2e3
- Interactor
- Botrytis cinereaBotrytis cinereae1pathogen
- Outcome
- ability to infect intact plant leavese4
- Direction
- loste4
- Qualifier
- could not form complete infection cushionse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Botrytis cinerea, a ubiquitous necrotrophic plant-pathogenic fungus, is responsible for grey mold and rot disease in a very wide range of plant species”
e3“deletion of Bcser2 or double deletion of Bcser1 and Bcser2 severely impaired the hyphal growth, sclerotial formation and conidiation of B. cinerea”
e4“lost the ability to infect intact plant leaves of Arabidopsis and tomato”
e6“could not form complete infection cushions”
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