Abstract
Regeneration of the coconut (Cocos nucifera L) through indirect somatic embryogenesis using 9 to 12 months embryos explants was established in Y3 medium supplemented with 100 to 250 µM 2,4 -dichlorophenoxyacetic acid (2, 4-D) alone and in combination with gibberellic acid (GA3), 6-benzylaminopurine (BAP) and thiadiazuron (TDZ). The highest callus induction (100%) was observed in medium containing 150 and 250 µM 2, 4-D + 5 µM BAP while the least was in 2, 4-D alone (16.7%) and in combination with 0.35 µM GA3 (0). The highest embryogenic calli were observed in medium with 125 µM 2, 4-D + 5 µM BAP (100%) and the least was in medium with 2, 4-D alone (16.7) and in combination with 0.35 µM GA3 (0). Multiple shoot induction was observed in medium supplemented with 10 µM kinetin, 10 µM BAP, 0.5 µM GA3, and 200 µM NAA. Maximum shoot elongation (1.667 cm) was in medium containing 10 µM BAP and the least (0.811 cm) was in medium with 5 µM BAP. Rooting was done in Y3 medium containing three levels of indole-3-butyric acid (IBA). The highest response was observed in Y3 medium supplemented with 5 µM IBA+ 0.5 µM GA3 both with respect to the number of roots 8.33 and root length 5.10 cm while the least was with 10 µM IBA+ 0.5 µM GA3 with regard to the number of roots 3.33 and root length 2.83 cm. Acclimatization was achieved in media prepared with soil: sand: manure ratio (3:1:1) with 25% survival rate and vermiculate medium with 8.3% survival rate. Hence, in vitro regeneration of coconut through somatic embryogenesis is a viable alternative for mass propagation. Key words: Cocos nucifera L., somatic embryogenesis, zygotic embryos.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 16
Claim · model-written summary
Callus induction peaked at 100% under 150 and 250 µM 2,4-D with BAP.
Verbatim
e0“The highest callus induction (100%) was observed in medium containing 150 and 250 µM 2, 4-D + 5 µM BAP while the least was in 2, 4-D alone (16.7%) and in combination with 0.35 µM GA3 (0).”
t0“coconut”
Claim · model-written summary
Callus induction under 2,4-D alone was 16.7%.
Verbatim
e0“The highest callus induction (100%) was observed in medium containing 150 and 250 µM 2, 4-D + 5 µM BAP while the least was in 2, 4-D alone (16.7%) and in combination with 0.35 µM GA3 (0).”
t0“coconut”
Claim · model-written summary
Callus induction was zero with 2,4-D plus GA3.
Verbatim
e0“The highest callus induction (100%) was observed in medium containing 150 and 250 µM 2, 4-D + 5 µM BAP while the least was in 2, 4-D alone (16.7%) and in combination with 0.35 µM GA3 (0).”
t0“coconut”
Claim · model-written summary
Embryogenic calli peaked at 100% with 125 µM 2,4-D plus BAP.
Verbatim
e0“The highest embryogenic calli were observed in medium with 125 µM 2, 4-D + 5 µM BAP (100%) and the least was in medium with 2, 4-D alone (16.7) and in combination with 0.35 µM GA3 (0).”
t0“coconut”
Claim · model-written summary
Embryogenic calli were 16.7 under 2,4-D alone.
Verbatim
e0“The highest embryogenic calli were observed in medium with 125 µM 2, 4-D + 5 µM BAP (100%) and the least was in medium with 2, 4-D alone (16.7) and in combination with 0.35 µM GA3 (0).”
t0“coconut”
Claim · model-written summary
Embryogenic calli were zero with GA3 combination.
Verbatim
e0“The highest embryogenic calli were observed in medium with 125 µM 2, 4-D + 5 µM BAP (100%) and the least was in medium with 2, 4-D alone (16.7) and in combination with 0.35 µM GA3 (0).”
t0“coconut”
Claim · model-written summary
A kinetin, BAP, GA3 and NAA medium induced multiple shoots.
Verbatim
e0“Multiple shoot induction was observed in medium supplemented with 10 µM kinetin, 10 µM BAP, 0.5 µM GA3, and 200 µM NAA.”
t0“coconut”
Claim · model-written summary
Shoot elongation peaked at 1.667 cm with 10 µM BAP.
Verbatim
e0“Maximum shoot elongation (1.667 cm) was in medium containing 10 µM BAP and the least (0.811 cm) was in medium with 5 µM BAP.”
t0“coconut”
Claim · model-written summary
Shoot elongation was lowest at 0.811 cm with 5 µM BAP.
Verbatim
e0“Maximum shoot elongation (1.667 cm) was in medium containing 10 µM BAP and the least (0.811 cm) was in medium with 5 µM BAP.”
t0“coconut”
Claim · model-written summary
The highest root number was 8.33 under 5 µM IBA plus GA3.
Verbatim
e0“The highest response was observed in Y3 medium supplemented with 5 µM IBA+ 0.5 µM GA3 both with respect to the number of roots 8.33 and root length 5.10 cm while the least was with 10 µM IBA+ 0.5 µM GA3 with regard to the number of roots 3.33 and root length 2.83 cm.”
t0“coconut”
Claim · model-written summary
The longest roots were 5.10 cm under 5 µM IBA plus GA3.
Verbatim
e0“The highest response was observed in Y3 medium supplemented with 5 µM IBA+ 0.5 µM GA3 both with respect to the number of roots 8.33 and root length 5.10 cm while the least was with 10 µM IBA+ 0.5 µM GA3 with regard to the number of roots 3.33 and root length 2.83 cm.”
t0“coconut”
Claim · model-written summary
The lowest root number was 3.33 under 10 µM IBA plus GA3.
Verbatim
e0“The highest response was observed in Y3 medium supplemented with 5 µM IBA+ 0.5 µM GA3 both with respect to the number of roots 8.33 and root length 5.10 cm while the least was with 10 µM IBA+ 0.5 µM GA3 with regard to the number of roots 3.33 and root length 2.83 cm.”
t0“coconut”
Claim · model-written summary
The shortest roots were 2.83 cm under 10 µM IBA plus GA3.
Verbatim
e0“The highest response was observed in Y3 medium supplemented with 5 µM IBA+ 0.5 µM GA3 both with respect to the number of roots 8.33 and root length 5.10 cm while the least was with 10 µM IBA+ 0.5 µM GA3 with regard to the number of roots 3.33 and root length 2.83 cm.”
t0“coconut”
Claim · model-written summary
Survival after acclimatization was 25% in soil, sand and manure.
Verbatim
e0“Acclimatization was achieved in media prepared with soil: sand: manure ratio (3:1:1) with 25% survival rate and vermiculate medium with 8.3% survival rate.”
t0“coconut”
Claim · model-written summary
Survival after acclimatization was 8.3% in vermiculate.
Verbatim
e0“Acclimatization was achieved in media prepared with soil: sand: manure ratio (3:1:1) with 25% survival rate and vermiculate medium with 8.3% survival rate.”
t0“coconut”
Claim · model-written summary
The authors recommend somatic embryogenesis for coconut mass propagation.
Verbatim
e0“in vitro regeneration of coconut through somatic embryogenesis is a viable alternative for mass propagation.”
t0“coconut”
The same questions in other papers
indole-3-butyric acid · growth · Cocos nucifera (coconut palm)Positive and negative results
1 in the opposite direction
- negative
Gibberellic acid · growth · Cocos nucifera (coconut palm)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
2,4-D · growth · Cocos nucifera (coconut palm)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →N-benzyladenine · growth · Cocos nucifera (coconut palm)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →in vitro · survival · Cocos nucifera (coconut palm)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.