Abstract
Coconut (Cocos nucifera L.) production has been declining in Mexico and other countries due to deadly diseases; therefore, massive production of palms resistant to diseases is needed for replanting. Production of somatic embryos and micropropagation could be a very useful technique to produce free disease coconut population. Explants (embryogenic-structures) of Pacific Tall 2 ecotype were cultured in an induction medium defined as I (semisolid Y3 medium containing 0.65 mM 2,4-dichlorophenoxyacetic acid) and treated with the cytokinin benzyladenine (BA) at 0, 25, 100 or 200 ,µM to evaluate its effect on embryogenic callus formation. The BA treatments did not affect the percentage of embryogenic callus formation. In order to evaluate the effect of the BA treatments on the late formation and germination of somatic embryos, the embryogenic calluses obtained in medium I were transferred to medium II (semisolid Y3 medium, containing 6 µM 2,4-dichlorophenoxyacetic acid and 300 ,µM BA). The best treatment was obtained from callus treated with 100 ,µM BA; 72 % of them produced 27 embryos per responding callus at 150 d while with 25 /µM BA and the control (0 µM BA) only 4 embryos were formed. The highest percentage (50 %) of calluses with germinating embryos occurred with 100 µM BA at 150 d; other treatments produced only 20 %. In this BA concentration, the number of germinating embryos per callus was 10, while with the other treatments only 2-3 embryos were formed at 150 d. The total yield of embryos and germinating embryos from 100 explants were 73.6 and 19.0, with 0 µM BA, and 753.2 and 249.0 with 100 µM BA. Pre treatment of coconut explants with BA (100 µM) and further treatment with 6 µM 2,4-D and 300 µM BA, improves the efficiency of coconut somatic embryo formation and germination.
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Claims 8
Claim · model-written summary
BA did not change the percentage of embryogenic callus formation.
- Taxon
- Coconute1
- Treatment
- BA treatmentse0
- Outcome
- percentage of embryogenic callus formatione0
- Direction
- did not affecte0
Not reportedComparator
Verbatim
e0“The BA treatments did not affect the percentage of embryogenic callus formation”
e1“Coconut (Cocos nucifera L.)”
Claim · model-written summary
At 150 days, 72% of calluses treated with 100 µM BA produced embryos.
Verbatim
e0“The best treatment was obtained from callus treated with 100 ,µM BA; 72 % of them produced 27 embryos per responding callus at 150 d while with 25 /µM BA and the control (0 µM BA) only 4 embryos were formed”
e1“Coconut (Cocos nucifera L.)”
Claim · model-written summary
At 150 days, 100 µM BA yielded 27 embryos per responding callus versus four with 25 µM BA or control.
Verbatim
e0“The best treatment was obtained from callus treated with 100 ,µM BA; 72 % of them produced 27 embryos per responding callus at 150 d while with 25 /µM BA and the control (0 µM BA) only 4 embryos were formed”
e1“Coconut (Cocos nucifera L.)”
Claim · model-written summary
Half of calluses had germinating embryos at 100 µM BA versus 20% with other treatments.
Verbatim
e0“The highest percentage (50 %) of calluses with germinating embryos occurred with 100 µM BA at 150 d; other treatments produced only 20 %”
e1“Coconut (Cocos nucifera L.)”
Claim · model-written summary
At 150 days, 100 µM BA produced 10 germinating embryos per callus versus 2 to 3 under other treatments.
- Taxon
- Coconute2
- Treatment
- 100 µM BAe1
- Outcome
- number of germinating embryos per calluse0
- Value
- 10e0
- Qualifier
- 2-3 embryose0
- Comparator
- other treatmentse0
Not reportedDirection
Verbatim
e0“In this BA concentration, the number of germinating embryos per callus was 10, while with the other treatments only 2-3 embryos were formed at 150 d”
e1“100 µM BA at 150 d”
e2“Coconut (Cocos nucifera L.)”
Claim · model-written summary
From 100 explants, total embryos increased from 73.6 at zero BA to 753.2 at 100 µM BA.
- Taxon
- Coconute1
- Treatment
- 100 µM BAe0
- Outcome
- total yield of embryose0
- Value
- 753.2e0
- Qualifier
- 73.6e0
- Comparator
- 0 µM BAe0
Not reportedDirection
Verbatim
e0“The total yield of embryos and germinating embryos from 100 explants were 73.6 and 19.0, with 0 µM BA, and 753.2 and 249.0 with 100 µM BA”
e1“Coconut (Cocos nucifera L.)”
Claim · model-written summary
From 100 explants, germinating embryos increased from 19.0 at zero BA to 249.0 at 100 µM BA.
- Taxon
- Coconute1
- Treatment
- 100 µM BAe0
- Outcome
- germinating embryose0
- Value
- 249.0e0
- Qualifier
- 19.0e0
- Comparator
- 0 µM BAe0
Not reportedDirection
Verbatim
e0“The total yield of embryos and germinating embryos from 100 explants were 73.6 and 19.0, with 0 µM BA, and 753.2 and 249.0 with 100 µM BA”
e1“Coconut (Cocos nucifera L.)”
Claim · model-written summary
Pretreating coconut explants with BA followed by 2,4-D and BA improves somatic embryo formation and germination.
Verbatim
e0“Pre treatment of coconut explants with BA (100 µM) and further treatment with 6 µM 2,4-D and 300 µM BA, improves the efficiency of coconut somatic embryo formation and germination”
The same questions in other papers
N-benzyladenine · growth · Cocos nucifera (coconut palm)Results agree
4 papers in the same direction as this paper
- positive
- positive
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.