Abstract
Zygotic embryos tissues have found to be more responsive explants for clonal propagation of coconut. In the present study, the feasibility of using zygotic embryos explants for clonal propagation of a local coconut variety MATAG F2 was assessed. Callus was induced by incorporating of cytokinin and auxin into the medium. The sliced embryos explants were immersed in 1 M maltose for 60 mins, then with 0.05 M maltose for 1 min and followed by 0.01 M maltose for 5 mins was the best for prevention of phenolic compounds excretion. The callus formation depended on the concentration of 2,4-D in the media and the best effect was observed with the high level (2,4-D and BAP) tested. Attempts at inducing multiple shoot from the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure.
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Claims 6
Claim · model-written summary
Callus was induced in coconut zygotic embryo explants by cytokinin and auxin in the medium.
Not reportedComparatorDirection
Verbatim
e1“Callus was induced by incorporating of cytokinin and auxin into the medium”
t0“Coconut (MATAG F2) Zygotic Embryos”
Claim · model-written summary
Maltose pretreatment of sliced embryo explants was the best for preventing phenolic compound excretion.
- Taxon
- Coconutt0
- Organ / stage
- sliced embryos explantse1
- Treatment
- 1 M maltose for 60 mins, then with 0.05 M maltose for 1 min and followed by 0.01 M maltose for 5 minse1
- Outcome
- prevention of phenolic compounds excretione1
- Direction
- was the beste1
Not reportedComparator
Verbatim
e1“The sliced embryos explants were immersed in 1 M maltose for 60 mins, then with 0.05 M maltose for 1 min and followed by 0.01 M maltose for 5 mins was the best for prevention of phenolic compounds excretion”
t0“Coconut (MATAG F2) Zygotic Embryos”
Claim · model-written summary
Callus formation in coconut depended on the 2,4-D concentration in the medium.
Not reportedDirection
Verbatim
e1“The callus formation depended on the concentration of 2,4-D in the media and the best effect was observed with the high level (2,4-D and BAP) tested”
t0“Coconut (MATAG F2) Zygotic Embryos”
Claim · model-written summary
High level of 2,4-D and BAP gave the best callus formation in coconut embryos.
- Taxon
- Coconutt0
- Treatment
- high level (2,4-D and BAP)e1
- Outcome
- callus formatione1
- Direction
- the best effecte1
Not reportedComparator
Verbatim
e1“The callus formation depended on the concentration of 2,4-D in the media and the best effect was observed with the high level (2,4-D and BAP) tested”
t0“Coconut (MATAG F2) Zygotic Embryos”
Claim · model-written summary
Multiple shoot induction from zygotic embryo callus of coconut was unsuccessful.
Verbatim
e1“Attempts at inducing multiple shoot from the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure”
t0“Coconut (MATAG F2) Zygotic Embryos”
Claim · model-written summary
Most coconut embryo callus became root structure.
Verbatim
e1“Attempts at inducing multiple shoot from the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure”
t0“Coconut (MATAG F2) Zygotic Embryos”
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