Abstract
Artificial seed production is now an outstanding technique for plant propagation and preservation, and has been applied on many plants. This study examines, for the first time the re growth of strawberry capsules under greenhouse conditions. Maximum shoot multiplication response (61.60 shoots) was obtained in Murashige and Skoog (MS) medium having 1.0 mg l-1 of Benzyl Adenine (BA), with an average leaf number of (143.2). However, the highest average shoot length was 6.20 and recorded at 1.5 mg l-1 KN. To protect shoot tips and produce synthetic seeds, gel capsules were standardized using a combination of sodium alginate and calcium nitrate or calcium chloride concentrations. Gel capsules were most effective for the formation of uniform and firm capsules when formed with a combination of 3% (w/v) sodium alginate and 100 mM calcium nitrate for 30 min. The highest percentage of shoot emergence was recorded when encapsulated in 3% Na-alginate dissolved in MS- salt solution containing 0.5mg mg l-1 BA and stored on MS basal medium containing 0.25 mg l-1 BA. The results demonstrated that the sugarcane bagasse could be used effectively as a substitute for agar during capsules storage. The capsules grown on MS media and sugarcane bagasse increase in shoot number, leaf number and shoot length, compared with agar-grown. In vitro storage of capsules was also evaluated by adding sugar alcohols, sorbitol or mannitol, to act as osmotic agents, to the media. Using 0.1M sorbitol or mannitol each to the storage media at 4°C after 12 months increased regrowth when inoculated on growth medium. Root induction was readily achieved within six weeks when shoots were transferred to MS medium supplemented with 0.5 mg l-1 IBA. Significant improvement in the in vitro rooting process, coupled with cost reduction, were obtained by the use of sugarcane bagasse as a substitute for the traditionally used agar-gel medium and Knop medium. Further, rooted plantlets were hardened by placing them in peatmoss + perlite (1:1, v/v) achieving a 75% survival rate in this potting mixture. Finally, the encapsulated shoot-tips coated in 3% Na-alginate, 0.2 or 0.3 g l-1 topsin and 0.5mg l-1 IBA were successfully established in the greenhouse.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
MS medium with 1.0 mg l-1 BA yielded a maximum of 61.60 shoots.
- Taxon
- Strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- 1.0 mg l-1 of Benzyl Adenine (BA)e0
- Outcome
- shoot multiplication responsee0
- Direction
- Maximume0
- Value
- 61.60 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Maximum shoot multiplication response (61.60 shoots) was obtained in Murashige and Skoog (MS) medium having 1.0 mg l-1 of Benzyl Adenine (BA)”
t0“Strawberry”
Claim · model-written summary
The same MS medium gave an average of 143.2 leaves.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- 1.0 mg l-1 of Benzyl Adenine (BA)e1
- Outcome
- leaf numbere0
- Value
- 143.2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with an average leaf number of (143.2).”
e1“1.0 mg l-1 of Benzyl Adenine (BA)”
t0“Strawberry”
Claim · model-written summary
At 1.5 mg l-1 KN, average shoot length was highest at 6.20.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- 1.5 mg l-1 KNe0
- Outcome
- average shoot lengthe0
- Direction
- higheste0
- Value
- 6.20e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest average shoot length was 6.20 and recorded at 1.5 mg l-1 KN.”
t0“Strawberry”
Claim · model-written summary
Capsules of 3% sodium alginate and 100 mM calcium nitrate formed uniform, firm capsules most effectively.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- 3% (w/v) sodium alginate and 100 mM calcium nitrate for 30 mine0
- Outcome
- uniform and firm capsulese0
- Value
- 3% (w/v) sodium alginate and 100 mM calcium nitratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gel capsules were most effective for the formation of uniform and firm capsules when formed with a combination of 3% (w/v) sodium alginate and 100 mM calcium nitrate for 30 min.”
t0“Strawberry”
Claim · model-written summary
The stated alginate and BA medium combination produced the highest shoot emergence percentage.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- 3% Na-alginate dissolved in MS- salt solution containing 0.5mg mg l-1 BAe0
- Outcome
- percentage of shoot emergencee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of shoot emergence was recorded when encapsulated in 3% Na-alginate dissolved in MS- salt solution containing 0.5mg mg l-1 BA and stored on MS basal medium containing 0.25 mg l-1 BA.”
t0“Strawberry”
Claim · model-written summary
Sugarcane bagasse functioned as an agar substitute during capsule storage.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- sugarcane bagassee0
- Outcome
- capsules storagee0
- Comparator
- agare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sugarcane bagasse could be used effectively as a substitute for agar during capsules storage.”
t0“Strawberry”
Claim · model-written summary
Capsules grown on MS media and sugarcane bagasse had more shoots, leaves and shoot length than agar-grown capsules.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- MS media and sugarcane bagassee0
- Outcome
- shoot number, leaf number and shoot lengthe0
- Direction
- increasee0
- Comparator
- agar-growne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capsules grown on MS media and sugarcane bagasse increase in shoot number, leaf number and shoot length, compared with agar-grown.”
t0“Strawberry”
Claim · model-written summary
Storage medium with 0.1M sorbitol at 4°C for 12 months increased capsule regrowth.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- 0.1M sorbitole0
- Outcome
- regrowthe0
- Direction
- increasede0
- Qualifier
- at 4°C after 12 monthse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using 0.1M sorbitol or mannitol each to the storage media at 4°C after 12 months increased regrowth when inoculated on growth medium.”
t0“Strawberry”
Claim · model-written summary
Storage medium with 0.1M mannitol at 4°C for 12 months increased capsule regrowth.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- mannitole0
- Outcome
- regrowthe0
- Direction
- increasede0
- Qualifier
- at 4°C after 12 monthse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using 0.1M sorbitol or mannitol each to the storage media at 4°C after 12 months increased regrowth when inoculated on growth medium.”
t0“Strawberry”
Claim · model-written summary
Transfer to MS medium with 0.5 mg l-1 IBA induced roots within six weeks.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- MS medium supplemented with 0.5 mg l-1 IBAe0
- Outcome
- Root inductione0
- Value
- six weekse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root induction was readily achieved within six weeks when shoots were transferred to MS medium supplemented with 0.5 mg l-1 IBA.”
t0“Strawberry”
Claim · model-written summary
Bagasse in place of agar-gel medium improved in vitro rooting.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- sugarcane bagassee0
- Outcome
- in vitro rooting processe0
- Direction
- improvemente0
- Comparator
- agar-gel medium and Knop mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant improvement in the in vitro rooting process, coupled with cost reduction, were obtained by the use of sugarcane bagasse as a substitute for the traditionally used agar-gel medium and Knop medium.”
t0“Strawberry”
Claim · model-written summary
Bagasse substitution reduced rooting medium cost.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- sugarcane bagassee0
- Outcome
- coste0
- Direction
- reductione0
- Comparator
- agar-gel medium and Knop mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant improvement in the in vitro rooting process, coupled with cost reduction, were obtained by the use of sugarcane bagasse as a substitute for the traditionally used agar-gel medium and Knop medium.”
t0“Strawberry”
Claim · model-written summary
Rooted plantlets had 75% survival in peatmoss and perlite at a 1:1 ratio.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- peatmoss + perlite (1:1, v/v)e0
- Outcome
- survival ratee0
- Value
- 75%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooted plantlets were hardened by placing them in peatmoss + perlite (1:1, v/v) achieving a 75% survival rate in this potting mixture.”
t0“Strawberry”
Claim · model-written summary
Encapsulated shoot tips coated with 0.2 g l-1 topsin and IBA established in the greenhouse.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- 3% Na-alginate, 0.2 or 0.3 g l-1 topsin and 0.5mg l-1 IBAe0
- Outcome
- successfully establishede0
- Setting
- greenhousee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the encapsulated shoot-tips coated in 3% Na-alginate, 0.2 or 0.3 g l-1 topsin and 0.5mg l-1 IBA were successfully established in the greenhouse.”
t0“Strawberry”
The same questions in other papers
N-benzyladenine · growth · Fragaria x ananassa (strawberry)Results differ
51 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
52 papers more in this group
See all 56 papers →glucitol · growth · Fragaria x ananassa (strawberry)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →N-benzyladenine · pest · Fragaria x ananassa (strawberry)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- positive
3 papers more in this group
See all 7 papers →mannitol · growth · Fragaria x ananassa (strawberry)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Fragaria x ananassa (strawberry)Positive and negative results
75 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- no effect
82 papers more in this group
See all 86 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.