Abstract
Abstract Present study was done to describe micropropagation protocol of Pomegranate (granatum Punica L) for two cultivar (Yamani and Wonderful), by testing their tolerance to sterilization duration with sodium hypochlorite solution at 1%, and investigation of suitability of of two media murashige and skoog (MS) and woody plant medium (WPM) during establishment stage in addition study the role of four type of Cytokinines BA (benzyl adenine), 2iP (isopentyl adenine), Kinetin(6-furfury lamino Purine) and TDZ (Thidiazuron) at different concentrations for two cultivars in multiplication stage and investigation of two type of Auxin NAA and IBA for rooting stage. the results appeared that sterilization at 10 min with sodium hypochlorite at 1% reduce contamination percentage to 6.2% and increased survival to 93.8%, the result proved WPM media was better than MS in increase shoot number, shoot length and leaf number was 1.17 shoot per explant, 5.02cm and 6.60 leaf per explant respectively. in the other way Yamani cultivar gave maximum value in comparison with wonderful for same characteristics. concerning the multiplication stage at 0.5 mg.l-1 BA achieved highest value of shoot number and leaf number (2.89 and 5) per explant respectively. while Kin gave best value of shoot length 4.12cm. moreover BA and 2iP at 1mg.l -1 , Kin at 5mg.l -1 and TDZ at1mg.l -1 achieved maximum values of same characteristics mentioned above. Auxins NAA (Naphthaleneacetic acid) and IBA (Indole-3-butyric acid) at 1mg.l-1 achieved highest root number was (4 and 3.17) per explant respectively.
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Claims 14
Claim · model-written summary
Sterilization for 10 min with 1% sodium hypochlorite reduced contamination to 6.2%.
- Taxon
- Punica granatum
Punica granatumt0 - Treatment
- sterilization at 10 min with sodium hypochlorite at 1%e1
- Outcome
- contamination percentagee1
- Direction
- reducee1
- Value
- 6.2%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“sterilization at 10 min with sodium hypochlorite at 1% reduce contamination percentage to 6.2% and increased survival to 93.8%”
Claim · model-written summary
Sterilization for 10 min with 1% sodium hypochlorite increased survival to 93.8%.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- sterilization at 10 min with sodium hypochlorite at 1%e1
- Outcome
- survivale1
- Direction
- increasede1
- Value
- 93.8%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“sterilization at 10 min with sodium hypochlorite at 1% reduce contamination percentage to 6.2% and increased survival to 93.8%”
Claim · model-written summary
WPM medium was better than MS for shoot number of pomegranate micropropagules, 1.17 shoots per explant.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- WPM mediae1
- Outcome
- shoot numbere1
- Direction
- bettere1
- Value
- 1.17 shoot per explante1
- Comparator
- MSe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“the result proved WPM media was better than MS in increase shoot number, shoot length and leaf number was 1.17 shoot per explant, 5.02cm and 6.60 leaf per explant respectively”
Claim · model-written summary
WPM medium was better than MS for shoot length of pomegranate micropropagules, 5.02 cm.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- WPM mediae1
- Outcome
- shoot lengthe1
- Direction
- bettere1
- Value
- 5.02cme1
- Comparator
- MSe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“the result proved WPM media was better than MS in increase shoot number, shoot length and leaf number was 1.17 shoot per explant, 5.02cm and 6.60 leaf per explant respectively”
Claim · model-written summary
WPM medium was better than MS for leaf number of pomegranate micropropagules, 6.60 leaves per explant.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- WPM mediae1
- Outcome
- leaf numbere1
- Direction
- bettere1
- Value
- 6.60 leaf per explante1
- Comparator
- MSe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“the result proved WPM media was better than MS in increase shoot number, shoot length and leaf number was 1.17 shoot per explant, 5.02cm and 6.60 leaf per explant respectively”
Claim · model-written summary
The Yamani cultivar gave the maximum values of shoot number, shoot length and leaf number compared with Wonderful.
- Taxon
- Punica granatumPunica granatumt0
- Material
- Yamanie1
- Outcome
- same characteristicse1
- Direction
- maximume1
- Comparator
- wonderfule1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“Yamani cultivar gave maximum value in comparison with wonderful for same characteristics”
Claim · model-written summary
Benzyladenine at 0.5 mg/l gave the highest shoot number per explant, 2.89.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- 0.5 mg.l-1 BAe1
- Outcome
- shoot numbere1
- Direction
- higheste1
- Value
- 2.89e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“at 0.5 mg.l-1 BA achieved highest value of shoot number and leaf number (2.89 and 5) per explant respectively”
Claim · model-written summary
Benzyladenine at 0.5 mg/l gave the highest leaf number per explant, 5.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- 0.5 mg.l-1 BAe1
- Outcome
- leaf numbere1
- Direction
- higheste1
- Value
- 5e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“at 0.5 mg.l-1 BA achieved highest value of shoot number and leaf number (2.89 and 5) per explant respectively”
Claim · model-written summary
Kinetin gave the best shoot length, 4.12 cm.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- Kine1
- Outcome
- shoot lengthe1
- Direction
- beste1
- Value
- 4.12cme1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“while Kin gave best value of shoot length 4.12cm”
Claim · model-written summary
BA and 2iP at 1 mg/l achieved the maximum values of shoot number, shoot length and leaf number.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- BA and 2iP at 1mg.l -1e1
- Outcome
- same characteristicse1
- Direction
- maximume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“BA and 2iP at 1mg.l -1 , Kin at 5mg.l -1 and TDZ at1mg.l -1 achieved maximum values of same characteristics”
Claim · model-written summary
Kinetin at 5 mg/l achieved the maximum values of shoot number, shoot length and leaf number.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- Kin at 5mg.l -1e1
- Outcome
- same characteristicse1
- Direction
- maximume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“BA and 2iP at 1mg.l -1 , Kin at 5mg.l -1 and TDZ at1mg.l -1 achieved maximum values of same characteristics”
Claim · model-written summary
TDZ at 1 mg/l achieved the maximum values of shoot number, shoot length and leaf number.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- TDZ at1mg.l -1e1
- Outcome
- same characteristicse1
- Direction
- maximume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“BA and 2iP at 1mg.l -1 , Kin at 5mg.l -1 and TDZ at1mg.l -1 achieved maximum values of same characteristics”
Claim · model-written summary
NAA at 1 mg/l gave the highest root number per explant, 4.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- NAAe1
- Outcome
- root numbere1
- Direction
- higheste1
- Value
- 4e1
- Qualifier
- 1mg.l-1e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“Auxins NAA (Naphthaleneacetic acid) and IBA (Indole-3-butyric acid) at 1mg.l-1 achieved highest root number was (4 and 3.17) per explant respectively”
Claim · model-written summary
IBA at 1 mg/l gave the highest root number per explant, 3.17.
- Taxon
- Punica granatumPunica granatumt0
- Treatment
- IBAe1
- Outcome
- root numbere1
- Direction
- higheste1
- Value
- 3.17e1
- Qualifier
- 1mg.l-1e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate Punica granatum L”
e1“Auxins NAA (Naphthaleneacetic acid) and IBA (Indole-3-butyric acid) at 1mg.l-1 achieved highest root number was (4 and 3.17) per explant respectively”
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