Abstract
The pomegranate fruits are of commercial values owing to their medicinal values. The plantation is popular due to the high yield at a low maintenance cost. Pomegranate is conventionally, propagated through lengthy and cumbersome grafting procedure. For commercial applications, a simple, reliable and fast method to propagate more number of plants in short duration is required. In this study we have attempted a rapid and efficient method of micro propagation of pomegranate (Punica granatum L. cv. Bhagwa) through axillary buds. Pomegranate cv Bhagwa was successfully mass propagated in tissue culture using nodal explants. 98% disease free plants were achieved by treating the explants in fungicide 0.1% (Captan – 50) and 0.1% mercuric chloride. Shoot proliferation was induced in nodal segments on Murashige and Skoog media containing various combinations of 6 – Benzylaminopurine (BAP) (0 – 2 mg L-1) and 1-naphthalacetic acid (NAA) (0 – 1 mg L-1). Maximum number of shoots (6.2) was produced in N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1 Amaximum shoot length of 7.7 cm with highest number of leaves (13) was recorded on the same medium. Regenerated nodal shoots were cut into segments to test the efficiency of rooting and it was observed that the apical region of nodal segment rooted better than the other regions, which has lead to more number of plants from a single nodal segment. Well rooted plants were acclimatized and hardened
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Claims 7
Claim · model-written summary
Bhagwa pomegranate was mass propagated from nodal explants.
- Taxon
- pomegranate
Punica granatume0 - Outcome
- mass propagated in tissue culturee0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pomegranate cv Bhagwa was successfully mass propagated in tissue culture using nodal explants.”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
Claim · model-written summary
Fungicide and mercuric chloride treatment produced 98% disease-free plants.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- fungicide 0.1% (Captan – 50) and 0.1% mercuric chloridee0
- Outcome
- disease free plantse0
- Value
- 98%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“98% disease free plants were achieved by treating the explants in fungicide 0.1% (Captan – 50) and 0.1% mercuric chloride.”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
Claim · model-written summary
N8 medium with BAP and NAA produced Maximum number of shoots.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1e0
- Outcome
- number of shootse0
- Direction
- Maximume0
- Value
- 6.2e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum number of shoots (6.2) was produced in N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1 Amaximum shoot length of 7.7 cm with highest number of leaves (13) was recorded on the same medium.”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
Claim · model-written summary
N8 medium with BAP and NAA produced Amaximum shoot length.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1e0
- Outcome
- shoot lengthe0
- Direction
- Amaximume0
- Value
- 7.7 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum number of shoots (6.2) was produced in N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1 Amaximum shoot length of 7.7 cm with highest number of leaves (13) was recorded on the same medium.”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
Claim · model-written summary
N8 medium with BAP and NAA produced highest number of leaves.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1e0
- Outcome
- number of leavese0
- Direction
- higheste0
- Value
- 13e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum number of shoots (6.2) was produced in N8 media with BAP 1.5 mg L-1 and 1-naphthalene acetic acid (NAA) 0.5 mg L-1 Amaximum shoot length of 7.7 cm with highest number of leaves (13) was recorded on the same medium.”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
Claim · model-written summary
Apical nodal segments rooted better than other regions.
- Taxon
- pomegranatePunica granatumt0
- Treatment
- apical region of nodal segmente0
- Outcome
- rootede0
- Direction
- bettere0
- Comparator
- other regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated nodal shoots were cut into segments to test the efficiency of rooting and it was observed that the apical region of nodal segment rooted better than the other regions, which has lead to more number of plants from a single nodal segment.”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
Claim · model-written summary
Rooted pomegranate plantlets were acclimatized and hardened.
- Taxon
- pomegranatePunica granatumt0
- Outcome
- acclimatized and hardenede0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Well rooted plants were acclimatized and hardened”
t0“In vitro shoot regeneration from nodal explants of pomegranate (Punica granatum L.) cv Bhagwa”
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