Abstract
Expression profiling for genes involved in Vitamin B6 (VitB6) biosynthesis was undertaken to delineate the involvement of de novo and salvage pathway induced by Bacillus subtilis CBR05 against, Xanthomonas campestris pv. vesicatoria in tomato. Pyridoxine biosynthesis (PDX) genes such as PDX1.2 and PDX1.3, were found to be overexpressed significantly at 72 hpi in B. subtilis and pyridoxine inoculated plants. Most significant upregulation was observed in the transcript profile of PDX1.3, which showed more than 12- fold increase in expression. Unfortunately, salt sensitive overlay4 (SOS4) profiling showed irregular expression which corroborates that SOS4 role in VitB6 biosynthesis needs further studies for deciphering a clear notion about their role in tomato. Antioxidant enzymes i.e., superoxide dismutase, catalase, polyphenol oxidase, and peroxidase activities clearly demonstrate escalation till 48 hpi and gets reduced in 72 hpi. Pot trials also confirm that B. subtilis compared to pyridoxine supplementation alone show plant disease resistance and elongated roots. The present study confirms that B. subtilis, as a versatile agent in eliciting induced systemic resistance regulated by de novo pathway as a model for plant defense against X. campestris pv. vesicatoria substantiated by VitB6 biosynthesis. Nevertheless, the study is preliminary and needs further evidence for affirming this phenomenon.
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Claims 8
Claim · model-written summary
PDX1.2 and PDX1.3 expression rose significantly after B. subtilis and pyridoxine inoculation.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- B. subtilis and pyridoxinee0
- Interactor
- B. subtilis
Bacillus subtilise0symbiont - Outcome
- PDX1.2 and PDX1.3e0
- Direction
- overexpressede0
- Qualifier
- 72 hpie0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pyridoxine biosynthesis (PDX) genes such as PDX1.2 and PDX1.3, were found to be overexpressed significantly at 72 hpi in B. subtilis and pyridoxine inoculated plants.”
t0“tomato”
Claim · model-written summary
PDX1.3 transcript expression rose more than twelvefold.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- B. subtilis and pyridoxinee1
- Interactor
- B. subtilisBacillus subtilise1symbiont
- Gene
- PDX1.3e0
- Outcome
- expressione0
- Direction
- increasee0
- Value
- more than 12- folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most significant upregulation was observed in the transcript profile of PDX1.3, which showed more than 12- fold increase in expression.”
e1“B. subtilis and pyridoxine inoculated plants”
t0“tomato”
Claim · model-written summary
SOS4 expression was irregular.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- SOS4e0
- Outcome
- irregular expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt sensitive overlay4 (SOS4) profiling showed irregular expression”
t0“tomato”
Claim · model-written summary
Antioxidant enzyme activities increased through 48 hpi.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- superoxide dismutase, catalase, polyphenol oxidase, and peroxidase activitiese0
- Direction
- escalatione0
- Qualifier
- 48 hpie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant enzymes i.e., superoxide dismutase, catalase, polyphenol oxidase, and peroxidase activities clearly demonstrate escalation till 48 hpi and gets reduced in 72 hpi.”
t0“tomato”
Claim · model-written summary
Antioxidant enzyme activities decreased at 72 hpi.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- superoxide dismutase, catalase, polyphenol oxidase, and peroxidase activitiese0
- Direction
- reducede0
- Qualifier
- 72 hpie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antioxidant enzymes i.e., superoxide dismutase, catalase, polyphenol oxidase, and peroxidase activities clearly demonstrate escalation till 48 hpi and gets reduced in 72 hpi.”
t0“tomato”
Claim · model-written summary
B. subtilis gave greater tomato disease resistance than pyridoxine alone.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- B. subtilise0
- Interactor
- Xanthomonas campestris pv. vesicatoria
_(25868012152).jpg?width=60)
Xanthomonas campestrist1pathogen - Second interactor
- B. subtilisBacillus subtilise0biocontrol_agent
- Outcome
- plant disease resistancee0
- Comparator
- pyridoxine supplementation alonee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(25868012152).jpg?width=500)
Verbatim
e0“B. subtilis compared to pyridoxine supplementation alone show plant disease resistance and elongated roots.”
t0“tomato”
t1“Xanthomonas campestris pv. vesicatoria”
Claim · model-written summary
B. subtilis produced longer tomato roots than pyridoxine alone.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- B. subtilise0
- Interactor
- B. subtilisBacillus subtilise0symbiont
- Outcome
- rootse0
- Direction
- elongatede0
- Comparator
- pyridoxine supplementation alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. subtilis compared to pyridoxine supplementation alone show plant disease resistance and elongated roots.”
t0“tomato”
Claim · model-written summary
The authors inferred induced systemic resistance through the de novo vitamin B6 pathway, with preliminary evidence.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- B. subtilise0
- Interactor
- X. campestris pv. vesicatoriaXanthomonas campestrise0pathogen
- Second interactor
- B. subtilisBacillus subtilise0biocontrol_agent
- Outcome
- induced systemic resistancee0
- Qualifier
- de novo pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. subtilis, as a versatile agent in eliciting induced systemic resistance regulated by de novo pathway as a model for plant defense against X. campestris pv. vesicatoria substantiated by VitB6 biosynthesis.”
e1“the study is preliminary and needs further evidence for affirming this phenomenon.”
t0“tomato”
The same questions in other papers
Bacillus subtilis · growth · Solanum lycopersicum (tomato)Positive and negative results
30 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- negative
30 papers more in this group
See all 34 papers →Bacillus subtilis · molecular: expression · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Bacillus subtilis · Xanthomonas campestris · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.