Abstract
An efficient system was established for high frequency embryogenesis and regeneration of a local variety, NEH Megha Rice1 (Oryza sativa L.). In the present study Callus was induced on MS (Murashige and Skoog) media supplemented with various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid), 0.5 g/L L-proline, 0.3 g/L casein hydrolysate 30 g/L sucrose and 8 g/L agar. NEH Megha Rice1 showed maximum callus induction frequency on MS medium supplemented with 2.5 mg/L 2,4-D. The regeneration efficiency from healthy embryogenic calli was investigated on MS medium supplemented with various concentrations of BAP (6-Benzylaminopurine) with a fixed concentration of NAA (Naphthalene acetic acid). The regeneration efficiency of calli was also investigated on three different agar concentrations. NEH Megha Rice1 showed maximum regeneration frequency on MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agar.
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Claims 2
Claim · model-written summary
NEH Megha Rice1 had maximum callus induction on MS medium with 2.5 mg/L 2,4-D.
- Taxon
- Rice
Oryza sativat0
- Material
- NEH Megha Rice1e0
- Treatment
- MS medium supplemented with 2.5 mg/L 2,4-De0
- Outcome
- callus induction frequencye0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NEH Megha Rice1 showed maximum callus induction frequency on MS medium supplemented with 2.5 mg/L 2,4-D.”
t0“Rice Seeds”
Claim · model-written summary
NEH Megha Rice1 had maximum regeneration on MS medium with 5mg/L BAP, 0.05mg/L NAA and 1.2 % agar.
- Taxon
- Rice
Oryza sativat0
- Material
- NEH Megha Rice1e0
- Treatment
- MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agare0
- Outcome
- regeneration frequencye0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NEH Megha Rice1 showed maximum regeneration frequency on MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agar.”
t0“Rice Seeds”
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