Paper · 2018 · International Journal of Current Trends in Science and Technology

Optimizing Tissue Culture Media for Efficient Callus Induction and Regeneration from Rice Seeds

Emica Mary Gatphoh; A. Pattanayak; Banshanlang Iangrai; et al.

2
claims
4
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

An efficient system was established for high frequency embryogenesis and regeneration of a local variety, NEH Megha Rice1 (Oryza sativa L.). In the present study Callus was induced on MS (Murashige and Skoog) media supplemented with various concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid), 0.5 g/L L-proline, 0.3 g/L casein hydrolysate 30 g/L sucrose and 8 g/L agar. NEH Megha Rice1 showed maximum callus induction frequency on MS medium supplemented with 2.5 mg/L 2,4-D. The regeneration efficiency from healthy embryogenic calli was investigated on MS medium supplemented with various concentrations of BAP (6-Benzylaminopurine) with a fixed concentration of NAA (Naphthalene acetic acid). The regeneration efficiency of calli was also investigated on three different agar concentrations. NEH Megha Rice1 showed maximum regeneration frequency on MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agar.

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Claims 2

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NEH Megha Rice1 had maximum callus induction on MS medium with 2.5 mg/L 2,4-D.

Material
NEH Megha Rice1e0
Treatment
MS medium supplemented with 2.5 mg/L 2,4-De0
Outcome
callus induction frequencye0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“NEH Megha Rice1 showed maximum callus induction frequency on MS medium supplemented with 2.5 mg/L 2,4-D.”

✓ located verbatim in the source · abstract, characters 376–480 · show in text ↑
t0

“Rice Seeds”

✓ located verbatim in the source · title, characters 85–95 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NEH Megha Rice1 had maximum regeneration on MS medium with 5mg/L BAP, 0.05mg/L NAA and 1.2 % agar.

Material
NEH Megha Rice1e0
Treatment
MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agare0
Outcome
regeneration frequencye0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“NEH Megha Rice1 showed maximum regeneration frequency on MS medium supplemented with 5mg/L BAP and 0.05mg/L NAA in 1.2 % agar.”

✓ located verbatim in the source · abstract, characters 800–926 · show in text ↑
t0

“Rice Seeds”

✓ located verbatim in the source · title, characters 85–95 · show in text ↑

1-naphthaleneacetic acid · growth · Oryza sativa (rice)Results differ

71 papers: 65 positive, 6 negative or no effect

64 papers in the same direction as this paper · 6 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

67 papers more in this group

See all 71 papers →

N-benzyladenine · growth · Oryza sativa (rice)Results differ

72 papers: 68 positive, 4 negative or no effect

67 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

68 papers more in this group

See all 72 papers →

agar · growth · Oryza sativa (rice)Positive and negative results

12 papers: 11 positive, 1 negative or no effect

10 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

2,4-D · growth · Oryza sativa (rice)Positive and negative results

107 papers: 102 positive, 5 negative or no effect

101 papers in the same direction as this paper · 5 in the opposite direction

  1. positive
  2. negative
  3. negative

103 papers more in this group

See all 107 papers →

in vitro · growth · Oryza sativa (rice)Positive and negative results

165 papers: 140 positive, 25 negative or no effect

139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

161 papers more in this group

See all 165 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.