Paper · 2021 · Saudi Journal of Biological Sciences

Effects of different gelling agents on the different stages of rice regeneration in two rice cultivars

Gehad M. Mohamed; Ahmed M. Amer; Neama H. Osman; et al.

6
claims
12
verbatim quotes
38
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Plant tissue culture technology offers a solution for meeting the increasing commercial demand on economically important plants such as rice, a widespread dietary staple. However, significant genotype-specific morphogenetic responses constitute a considerable on rice regeneration in plant biotechnology contexts. Aside from genotype dependency, the components of the nutrient media including gelling agents have an important impact on regeneration efficiency. The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178. Media solidified with varying concentrations of a variety of gelling agents (agar, bacto agar, gelrite and phytagel) were tested for their impact on the frequency of callus induction, shoot regeneration and rooting. The results indicated gellan gum (gelrite and phytagel) was superior to agar products (agar and bacto agar) for callus induction. By contrast, no significant differences were found between different gelling agents for shoot regeneration. Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar. The Sakha104 cultivar showed better responses than Giza 178 for callus induction and similar performance to the Giza 178 cultivar for root regeneration irrespective of the gelling agent. This work provides insights into the impact of different gelling agents on the morphogenetic response of two rice cultivars and can be used to help maximize the frequency of rice regeneration.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Gellan gum (gelrite and phytagel) was superior to agar products for callus induction in rice.

Material
Sakha104 and Giza178e0
Treatment
gellan gum (gelrite and phytagel)e1
Outcome
callus inductione1
Direction
superiore1
Comparator
agar products (agar and bacto agar)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178”

✓ located verbatim in the source · abstract, characters 461–617 · show in text ↑
e1

“The results indicated gellan gum (gelrite and phytagel) was superior to agar products (agar and bacto agar) for callus induction”

✓ located verbatim in the source · abstract, characters 835–963 · show in text ↑
Associationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

No significant differences were found between gelling agents for shoot regeneration in rice.

Treatment
different gelling agentse2
Outcome
shoot regeneratione2
Direction
no significant differencese2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178”

✓ located verbatim in the source · abstract, characters 461–617 · show in text ↑
e2

“no significant differences were found between different gelling agents for shoot regeneration”

✓ located verbatim in the source · abstract, characters 978–1071 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Gellan gum led to significantly higher root regeneration than agar in rice.

Material
Sakha104 and Giza178e0
Treatment
Gellan gume3
Outcome
root regeneratione3
Direction
significantly highere3
Comparator
agare3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178”

✓ located verbatim in the source · abstract, characters 461–617 · show in text ↑
e3

“Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar”

✓ located verbatim in the source · abstract, characters 1073–1191 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Media solidified with bacto agar led to significantly higher root regeneration than agar in rice.

Material
Sakha104 and Giza178e0
Treatment
media solidified with bacto agare3
Outcome
root regeneratione3
Direction
significantly highere3
Comparator
agare3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178”

✓ located verbatim in the source · abstract, characters 461–617 · show in text ↑
e3

“Gellan gum and media solidified with bacto agar were found to lead to significantly higher root regeneration than agar”

✓ located verbatim in the source · abstract, characters 1073–1191 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Cultivar Sakha104 showed better callus induction responses than Giza 178.

Material
Sakha104e4
Outcome
callus inductione4
Direction
better responsese4
Comparator
Giza 178e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178”

✓ located verbatim in the source · abstract, characters 461–617 · show in text ↑
e4

“The Sakha104 cultivar showed better responses than Giza 178 for callus induction”

✓ located verbatim in the source · abstract, characters 1193–1273 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Cultivar Sakha104 showed similar root regeneration performance to Giza 178.

Outcome
root regeneratione5
Direction
similar performancee5
Comparator
Giza 178e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The current study explores the effect of different gelling agents on various stages of rice regeneration in two Egyptian rice cultivars-Sakha104 and Giza178”

✓ located verbatim in the source · abstract, characters 461–617 · show in text ↑
e5

“similar performance to the Giza 178 cultivar for root regeneration”

✓ located verbatim in the source · abstract, characters 1278–1344 · show in text ↑

agar · growth · Oryza sativa (rice)Positive and negative results

12 papers: 11 positive, 1 negative or no effect

10 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

in vitro · growth · Oryza sativa (rice)Positive and negative results

165 papers: 140 positive, 25 negative or no effect

139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. positive
  3. negative

161 papers more in this group

See all 165 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.