Abstract
Cassava constitutes a greater part of the diet of most Africans south of the Sahara and the demand for the crops has been on a steady increase. Agriculturally, cassava performs very well, but the roots and leaves contain cyanogenic glycosides that are dangerous to human health. As such, cassava cultivars with low cyanide content offer great opportunities for domestic and industrial utilization. However the major constraints to the production of low cyanide cassava cultivar are susceptibility to diseases such as cassava mosaic virus (CMV) and non-availability of disease free planting materials. Application of biotechnology such as plant tissue culture could have a significant impact in the production large amount disease free planting materials. The effects of 2, 4-D and Picloram on callus induction and primary somatic embryogenesis in the low cyanide cassava varieties was evaluated. The result indicated a significant increase in percentage callus induction with increase in the concentration of 2, 4-D with 4mg/L producing the highest callus induction frequency of 72.21%. Similarly on media supplemented with Picloram, 7mg/L produced the highest callus induction frequency (73.11%) and frequency decreases with corresponding decrease in Picloram concentration. For the formation of somatic embryos, the result indicated a significant increase in number of primary embryos with increase in the concentrations of 2, 4-D or Picloram. This protocol presents a reproducible system for generation totipotent culture that could be used in genetic improvement of cassava and mass production of disease-free planting materials.Keywords: Cassava, Auxin, Callogenesis, Somatic embryogenesis
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Claims 7
Claim · model-written summary
Increasing 2, 4-D concentration significantly increased callus induction percentage.
- Taxon
- cassava
Manihot esculentat0 - Treatment
- increase in the concentration of 2, 4-De0
- Outcome
- percentage callus inductione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The result indicated a significant increase in percentage callus induction with increase in the concentration of 2, 4-D with 4mg/L producing the highest callus induction frequency of 72.21%.”
t0“cassava”
Claim · model-written summary
2, 4-D at 4mg/L produced the highest callus induction frequency, 72.21%.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 2, 4-D with 4mg/Le0
- Outcome
- callus induction frequencye0
- Direction
- higheste0
- Value
- 72.21%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2, 4-D with 4mg/L producing the highest callus induction frequency of 72.21%.”
t0“cassava”
Claim · model-written summary
Picloram at 7mg/L produced the highest callus induction frequency, 73.11%.
- Taxon
- cassavaManihot esculentat0
- Treatment
- media supplemented with Picloram, 7mg/Le0
- Outcome
- callus induction frequencye0
- Direction
- higheste0
- Value
- 73.11%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similarly on media supplemented with Picloram, 7mg/L produced the highest callus induction frequency (73.11%)”
t0“cassava”
Claim · model-written summary
Callus induction frequency decreased as Picloram concentration decreased.
- Taxon
- cassavaManihot esculentat0
- Treatment
- decrease in Picloram concentratione0
- Outcome
- callus induction frequencye1
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“frequency decreases with corresponding decrease in Picloram concentration.”
e1“the highest callus induction frequency (73.11%)”
t0“cassava”
Claim · model-written summary
Increasing 2, 4-D concentration significantly increased primary embryo number.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 2, 4-De0
- Outcome
- number of primary embryose0
- Direction
- increasee0
- Qualifier
- increase in the concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For the formation of somatic embryos, the result indicated a significant increase in number of primary embryos with increase in the concentrations of 2, 4-D or Picloram.”
t0“cassava”
Claim · model-written summary
Increasing Picloram concentration significantly increased primary embryo number.
- Taxon
- cassavaManihot esculentat0
- Treatment
- Piclorame0
- Outcome
- number of primary embryose0
- Direction
- increasee0
- Qualifier
- increase in the concentrationse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For the formation of somatic embryos, the result indicated a significant increase in number of primary embryos with increase in the concentrations of 2, 4-D or Picloram.”
t0“cassava”
Claim · model-written summary
The protocol provides a reproducible totipotent culture system potentially useful for cassava improvement and disease-free planting material production.
- Taxon
- cassavaManihot esculentae0
- Outcome
- reproducible system for generation totipotent culturee0
- Qualifier
- could be used in genetic improvement of cassava and mass production of disease-free planting materialse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This protocol presents a reproducible system for generation totipotent culture that could be used in genetic improvement of cassava and mass production of disease-free planting materials.”
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See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.