Abstract
The effect of different concentrations of thidiazuron (TDZ), benzyl amino purine (BAP), kinetin and 2,4-dichlorophenoxy acetic acid (2, 4-D) on callus induction in three elite cassava cultivars (agric-rouge, atinwewe and agblehoundo) was evaluated. Leaf explants harvested from greenhouse-grown cassava were sterilised using different concentrations of commercial bleach commonly called Jik (3.85% NaOCl) at different time intervals. The highest number (94%) of clean explants was obtained when 2% (v/v) Jik was used for 15 min. The explants were cultured in half MS media supplemented with different growth regulators TDZ, BAP, kinetin 2, 4-D, 100 mg/l myo inositol, 2% sucrose and gelled with 0.3% phytagel. Callus formation was observed from the cut edges of the leaves in all cultivars after 10 days in medium supplemented with TDZ, 12 days in BAP medium, and 15 days in kinetin medium. There were significant (p < 0.05) differences in callus formation among all cytokinins types and concentrations. However, there were no significant differences in callus formation in different 2,4-D concentrations. All 2,4-D concentrations produced 100% callus in all the cultivars. However, 2,4-D at 2 µM significantly produced the highest (2.48±0.30) callus weight in cultivar atinwewe. Furthermore, simple sequence repeats (SSR) and sequence-characterized amplified region of the induced calli on TDZ and 2,4-D media indicated the loss of CMD2 gene among induced calli compared to the mother plants. Key words: Cassava, simple sequence repeats (SSR) and sequence-characterized amplified region (SCAR), genetic stability, callus, cytokinins, auxins.
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Claims 9
Claim · model-written summary
The highest number (94%) of clean cassava leaf explants was obtained with 2% (v/v) Jik for 15 min.
- Taxon
- cassava
Manihot esculentat0 - Treatment
- 2% (v/v) Jike0
- Outcome
- clean explantse0
- Direction
- higheste0
- Value
- 94%e0
- Qualifier
- 15 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“The highest number (94%) of clean explants was obtained when 2% (v/v) Jik was used for 15 min”
Claim · model-written summary
Callus formation was observed in all cultivars after 10 days in medium supplemented with TDZ.
- Taxon
- cassavaManihot esculentat0
- Treatment
- medium supplemented with TDZe0
- Outcome
- Callus formatione0
- Value
- 10 dayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“Callus formation was observed from the cut edges of the leaves in all cultivars after 10 days in medium supplemented with TDZ, 12 days in BAP medium, and 15 days in kinetin medium”
Claim · model-written summary
Callus formation was observed in all cultivars after 12 days in BAP medium.
- Taxon
- cassavaManihot esculentat0
- Treatment
- BAP mediume0
- Outcome
- Callus formatione0
- Value
- 12 dayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“Callus formation was observed from the cut edges of the leaves in all cultivars after 10 days in medium supplemented with TDZ, 12 days in BAP medium, and 15 days in kinetin medium”
Claim · model-written summary
Callus formation was observed in all cultivars after 15 days in kinetin medium.
- Taxon
- cassavaManihot esculentat0
- Treatment
- kinetin mediume0
- Outcome
- Callus formatione0
- Value
- 15 dayse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“Callus formation was observed from the cut edges of the leaves in all cultivars after 10 days in medium supplemented with TDZ, 12 days in BAP medium, and 15 days in kinetin medium”
Claim · model-written summary
There were significant (p < 0.05) differences in callus formation among all cytokinin types and concentrations.
- Taxon
- cassavaManihot esculentat0
- Treatment
- cytokinins types and concentrationse0
- Outcome
- callus formatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“There were significant (p < 0.05) differences in callus formation among all cytokinins types and concentrations”
Claim · model-written summary
There were no significant differences in callus formation among different 2,4-D concentrations.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 2,4-D concentrationse0
- Outcome
- callus formatione0
- Direction
- no significant differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“no significant differences in callus formation in different 2,4-D concentrations”
Claim · model-written summary
All 2,4-D concentrations produced 100% callus in all the cultivars.
- Taxon
- cassavaManihot esculentat0
- Treatment
- 2,4-D concentrationse0
- Outcome
- calluse0
- Value
- 100%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“All 2,4-D concentrations produced 100% callus in all the cultivars”
Claim · model-written summary
In cultivar atinwewe, 2,4-D at 2 uM significantly produced the highest callus weight (2.48 +/- 0.30).
- Taxon
- cassavaManihot esculentat0
- Material
- atinwewee0
- Treatment
- 2,4-D at 2 µMe0
- Outcome
- callus weighte0
- Direction
- higheste0
- Value
- 2.48±0.30e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“2,4-D at 2 µM significantly produced the highest (2.48±0.30) callus weight in cultivar atinwewe”
Claim · model-written summary
SSR and SCAR markers indicated loss of the CMD2 gene among induced calli on TDZ and 2,4-D media compared with the mother plants.
- Taxon
- cassavaManihot esculentat0
- Treatment
- TDZ and 2,4-D mediae0
- Gene
- CMD2e0
- Outcome
- loss of CMD2 genee0
- Comparator
- mother plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cassava”
e0“of the induced calli on TDZ and 2,4-D media indicated the loss of CMD2 gene among induced calli compared to the mother plants”
The same questions in other papers
2,4-D · molecular: expression · Manihot esculenta (cassava)Positive and negative results
1 in the opposite direction
- positive
2,4-D · growth · Manihot esculenta (cassava)Results agree
10 papers in the same direction as this paper
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →in vitro · molecular: expression · Manihot esculenta (cassava)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.