Abstract
A method for the induction of somatic embryogenesis in eight cassava genotypes from northeastern Brazil is described. The explants used were shoot apexes isolated both from in vitro grown plants and from shoots that sprouted from stem cuttings. Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations. Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L. The method could be used not only for the mass production of plants of the cassava genotypes, but also to generate explants (green cotyledons of somatic embryos) as themselves excellent targets for genetic transformation.
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Claims 2
Claim · model-written summary
Picloram induced primary somatic embryogenesis at high frequencies over a wide concentration range.
- Taxon
- cassava
Manihot esculentat0 - Treatment
- piclorame0
- Outcome
- Somatic embryogenesise0
- Direction
- achievede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryogenesis was achieved in high frequencies by the addition in the induction medium of the auxin picloram over a wide range of concentrations.”
t0“cassava genotypes”
Claim · model-written summary
Green cotyledons produced cyclic secondary somatic embryos on medium with 12 mg/L picloram.
- Taxon
- cassavaManihot esculentat0
- Organ / stage
- Green cotyledons of primary somatic embryose0
- Treatment
- picloram at 12 mg/Le0
- Outcome
- somatic (cyclic) secondary embryogenesise0
- Value
- 12 mg/Le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Green cotyledons of primary somatic embryos were used as explants to induce somatic (cyclic) secondary embryogenesis in an inducing medium supplemented with picloram at 12 mg/L.”
t0“cassava genotypes”
The same questions in other papers
picloram · growth · Manihot esculenta (cassava)Results agree
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- positive
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