Abstract
Experiments have been performed to induce callus formation and organogenesis in anther culture of cassava (Manihot esculenta Crantz). Callusing was achieved on a modified Murashige and Skoog medium (MSB) supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2.4-D). No callus was formed from anthers pretreated at 4 °C for more than 48 h or on a medium containing 4g/ℓ activated charcoal. Callus on MSB with 4.44–8.88 μM BAP alone formed roots only. BAP (8.88 μM) in combination with α-naphthalene acetic acid (NAA) (10.74 μM) resulted in chlorophyll formation in callus. Abscisic acid (ABA) acted as an antagonist to NAA in reducing the frequency of callus greening when the latter was applied jointly with BAP. Chromosome counts of mitotic figures from callus cells ranged from 34 to 38 indicating that the calli were derived from the somatic tissues of the anthers.
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Claims 7
Claim · model-written summary
MSB with 4.44 μM BAP and 4.52 μM 2,4-D induced cassava anther callus.
- Taxon
- cassava
Manihot esculentat0 - Organ / stage
- antherst0
- Treatment
- 4.44 μM 6-benzylaminopurine (BAP) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2.4-D)e0
- Outcome
- Callusinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callusing was achieved on a modified Murashige and Skoog medium (MSB) supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 4.52 μM 2,4-dichlorophenoxyacetic acid (2.4-D)”
t0“cassava anthers”
Claim · model-written summary
Pretreatment at 4 °C for over 48 hours prevented callus formation.
- Taxon
- cassavaManihot esculentat0
- Organ / stage
- antherse0
- Treatment
- pretreated at 4 °C for more than 48 he0
- Outcome
- calluse0
- Direction
- No callus was formede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No callus was formed from anthers pretreated at 4 °C for more than 48 h”
t0“cassava anthers”
Claim · model-written summary
A medium containing 4g/ℓ activated charcoal prevented callus formation.
- Taxon
- cassavaManihot esculentat0
- Treatment
- medium containing 4g/ℓ activated charcoale0
- Outcome
- calluse0
- Direction
- No callus was formede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No callus was formed from anthers pretreated at 4 °C for more than 48 h or on a medium containing 4g/ℓ activated charcoal”
t0“cassava anthers”
Claim · model-written summary
Callus on MSB with 4.44–8.88 μM BAP alone formed only roots.
- Taxon
- cassavaManihot esculentat0
- Treatment
- MSB with 4.44–8.88 μM BAP alonee0
- Outcome
- rootse0
- Direction
- formede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus on MSB with 4.44–8.88 μM BAP alone formed roots only”
t0“cassava anthers”
Claim · model-written summary
8.88 μM BAP with 10.74 μM NAA induced chlorophyll formation in callus.
- Taxon
- cassavaManihot esculentat0
- Treatment
- BAP (8.88 μM) in combination with α-naphthalene acetic acid (NAA) (10.74 μM)e0
- Outcome
- chlorophyll formation in calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP (8.88 μM) in combination with α-naphthalene acetic acid (NAA) (10.74 μM) resulted in chlorophyll formation in callus”
t0“cassava anthers”
Claim · model-written summary
ABA reduced callus greening frequency when combined with NAA and BAP.
- Taxon
- cassavaManihot esculentat0
- Treatment
- Abscisic acid (ABA)e0
- Outcome
- frequency of callus greeninge0
- Direction
- reducinge0
- Qualifier
- when the latter was applied jointly with BAPe0
- Comparator
- NAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid (ABA) acted as an antagonist to NAA in reducing the frequency of callus greening when the latter was applied jointly with BAP”
t0“cassava anthers”
Claim · model-written summary
Cassava anther callus cells had 34 to 38 chromosomes, indicating somatic tissue origin.
- Taxon
- cassavaManihot esculentat0
- Organ / stage
- callus cellse0
- Outcome
- Chromosome countse0
- Value
- 34 to 38e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromosome counts of mitotic figures from callus cells ranged from 34 to 38 indicating that the calli were derived from the somatic tissues of the anthers”
t0“cassava anthers”
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