Abstract
Ubiquitination and deubiquitination are reversible processes that play crucial roles in regulating organ size in plants. However, information linking deubiquitination and seed size in rice (Oryza sativa) is limited. Here, we characterized a dominant large-grain mutant, large grain1-D (lg1-D), with a 30.8% increase in seed width and a 34.5% increase in 1,000-grain weight relative to the wild type. The lg1-D mutant had more cells oriented in the lateral direction of the spikelet hull compared with the wild type. Map-based cloning showed that LG1 encodes a constitutively expressed ubiquitin-specific protease15 (OsUBP15) that possesses deubiquitination activity in vitro. Loss-of-function and down-regulated expression of OsUBP15 produced narrower and smaller grains than the control. A set of in vivo experiments indicated that the mutant Osubp15 had enhanced protein stability relative to wild-type OsUBP15. Further experiments verified that OsDA1 directly interacted with OsUBP15. Genetic data indicated that OsUBP15 and GRAIN WIDTH 2 (GW2) were not independent in regulating grain width and size. In summary, we identified OsUBP15 as a positive regulator of grain width and size in rice and provide a promising strategy for improvement of grain yield by pyramiding OsUBP15 and gw2.
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Claims 9
Claim · model-written summary
lg1-D increased seed width by 30.8% versus wild type.
- Taxon
- rice
Oryza sativat0 - Material
- lg1-De0
- Outcome
- seed widthe0
- Direction
- increasee0
- Value
- 30.8%e0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“large grain1-D (lg1-D), with a 30.8% increase in seed width and a 34.5% increase in 1,000-grain weight relative to the wild type.”
t0“Rice”
Claim · model-written summary
lg1-D increased 1,000-grain weight by 34.5% versus wild type.
- Taxon
- riceOryza sativat0
- Material
- lg1-De0
- Outcome
- 1,000-grain weighte0
- Direction
- increasee0
- Value
- 34.5%e0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“large grain1-D (lg1-D), with a 30.8% increase in seed width and a 34.5% increase in 1,000-grain weight relative to the wild type.”
t0“rice (Oryza sativa)”
Claim · model-written summary
lg1-D had more laterally oriented spikelet hull cells than wild type.
- Taxon
- riceOryza sativat0
- Material
- lg1-De0
- Outcome
- cells oriented in the lateral direction of the spikelet hulle0
- Direction
- moree0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The lg1-D mutant had more cells oriented in the lateral direction of the spikelet hull compared with the wild type.”
t0“rice (Oryza sativa)”
Claim · model-written summary
LG1 encodes OsUBP15 with in vitro deubiquitination activity.
- Taxon
- riceOryza sativat0
- Gene
- OsUBP15e0
- Outcome
- deubiquitination activity in vitroe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LG1 encodes a constitutively expressed ubiquitin-specific protease15 (OsUBP15) that possesses deubiquitination activity in vitro.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Loss of OsUBP15 function or expression produced narrower and smaller grains.
- Taxon
- riceOryza sativat0
- Treatment
- Loss-of-function and down-regulated expression of OsUBP15e0
- Gene
- OsUBP15e0
- Outcome
- grainse0
- Direction
- narrower and smallere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function and down-regulated expression of OsUBP15 produced narrower and smaller grains than the control.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Mutant Osubp15 protein was more stable than wild-type OsUBP15.
- Taxon
- riceOryza sativat0
- Gene
- Osubp15e0
- Outcome
- protein stabilitye0
- Direction
- enhancede0
- Comparator
- wild-type OsUBP15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutant Osubp15 had enhanced protein stability relative to wild-type OsUBP15.”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsDA1 directly interacted with OsUBP15.
- Taxon
- riceOryza sativat0
- Gene
- OsDA1e0
- Outcome
- directly interacted with OsUBP15e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsDA1 directly interacted with OsUBP15.”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsUBP15 and GW2 were not independent in grain-size regulation.
- Taxon
- riceOryza sativat0
- Gene
- OsUBP15e0
- Outcome
- grain width and sizee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsUBP15 and GRAIN WIDTH 2 (GW2) were not independent in regulating grain width and size.”
t0“rice (Oryza sativa)”
Claim · model-written summary
Authors propose pyramiding OsUBP15 and gw2 to improve yield.
- Taxon
- riceOryza sativat0
- Gene
- OsUBP15e0
- Outcome
- improvement of grain yield by pyramiding OsUBP15 and gw2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“provide a promising strategy for improvement of grain yield by pyramiding OsUBP15 and gw2.”
t0“rice (Oryza sativa)”
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