Abstract
Achieving increased grain productivity has long been the overriding focus of cereal breeding programs. The ideotype approach has been used to improve rice yield potential at the International Rice Research Institute and in China. However, the genetic basis of yield-related traits in rice remains unclear. Here, we show that a major quantitative trait locus, qNPT1, acts through the determination of a 'new plant type' (NPT) architecture characterized by fewer tillers, sturdier culms and larger panicles, and it encodes a deubiquitinating enzyme with homology to human OTUB1. Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number, increased grain number, enhanced grain weight and a consequent increase in grain yield in rice. Unlike human OTUB1, OsOTUB1 can cleave both K48- and K63-linked polyubiquitin. OsOTUB1 interacts with the E2 ubiquitin-conjugating protein OsUBC13 and the squamosa promoter-binding protein-like transcription factor OsSPL14. OsOTUB1 and OsSPL14 share common target genes, and their physical interaction limits K63-linked ubiquitination (K63Ub) of OsSPL14, which in turn promotes K48Ub-dependent proteasomal degradation of OsSPL14. Conversely, loss-of-function of OsOTUB1 is correlated with the accumulation of high levels of OsSPL14, resulting in the NPT architecture. We also demonstrated that pyramiding of high-yielding npt1 and dep1-1 alleles provides a new strategy for increasing rice yield potential above what is currently achievable.
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Claims 13
Claim · model-written summary
qNPT1 encodes an OTUB1-like deubiquitinating enzyme and determines new plant type rice architecture.
- Taxon
- rice
Oryza sativat0 - Gene
- qNPT1e0
- Outcome
- 'new plant type' (NPT) architecturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a major quantitative trait locus, qNPT1, acts through the determination of a 'new plant type' (NPT) architecture characterized by fewer tillers, sturdier culms and larger panicles, and it encodes a deubiquitinating enzyme with homology to human OTUB1”
t0“rice”
Claim · model-written summary
Downregulation of OsOTUB1 enhances rice meristematic activity.
- Taxon
- riceOryza sativat0
- Treatment
- Downregulation of OsOTUB1e0
- Gene
- OsOTUB1e0
- Outcome
- meristematic activitye0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of OsOTUB1 enhances meristematic activity”
t0“rice”
Claim · model-written summary
Downregulation of OsOTUB1 reduces rice tiller number.
- Taxon
- riceOryza sativat0
- Treatment
- Downregulation of OsOTUB1e0
- Gene
- OsOTUB1e0
- Outcome
- tiller numbere0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number”
t0“rice”
Claim · model-written summary
Downregulation of OsOTUB1 increases rice grain number.
- Taxon
- riceOryza sativat0
- Treatment
- Downregulation of OsOTUB1e0
- Gene
- OsOTUB1e0
- Outcome
- grain numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number, increased grain number”
t0“rice”
Claim · model-written summary
Downregulation of OsOTUB1 enhances rice grain weight.
- Taxon
- riceOryza sativat0
- Treatment
- Downregulation of OsOTUB1e0
- Gene
- OsOTUB1e0
- Outcome
- grain weighte0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number, increased grain number, enhanced grain weight”
t0“rice”
Claim · model-written summary
Downregulation of OsOTUB1 increases rice grain yield.
- Taxon
- riceOryza sativae0
- Treatment
- Downregulation of OsOTUB1e0
- Gene
- OsOTUB1e0
- Outcome
- grain yielde0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number, increased grain number, enhanced grain weight and a consequent increase in grain yield in rice.”
Claim · model-written summary
OsOTUB1 cleaves K48- and K63-linked polyubiquitin.
- Taxon
- riceOryza sativat0
- Gene
- OsOTUB1e0
- Outcome
- K48- and K63-linked polyubiquitine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsOTUB1 can cleave both K48- and K63-linked polyubiquitin.”
t0“rice”
Claim · model-written summary
OsOTUB1 interacts with OsUBC13 and OsSPL14.
- Taxon
- riceOryza sativat0
- Gene
- OsOTUB1e0
- Outcome
- OsUBC13 and the squamosa promoter-binding protein-like transcription factor OsSPL14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsOTUB1 interacts with the E2 ubiquitin-conjugating protein OsUBC13 and the squamosa promoter-binding protein-like transcription factor OsSPL14.”
t0“rice”
Claim · model-written summary
OsOTUB1 and OsSPL14 share common target genes.
- Taxon
- riceOryza sativat0
- Gene
- OsOTUB1 and OsSPL14e0
- Outcome
- common target genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsOTUB1 and OsSPL14 share common target genes”
t0“rice”
Claim · model-written summary
Interaction of OsOTUB1 with OsSPL14 limits K63-linked ubiquitination of OsSPL14.
- Taxon
- riceOryza sativat0
- Gene
- OsSPL14e0
- Outcome
- K63-linked ubiquitination (K63Ub) of OsSPL14e0
- Direction
- limitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their physical interaction limits K63-linked ubiquitination (K63Ub) of OsSPL14”
t0“rice”
Claim · model-written summary
Limited K63 ubiquitination promotes K48 ubiquitin-dependent proteasomal degradation of OsSPL14.
- Taxon
- riceOryza sativat0
- Gene
- OsSPL14e0
- Outcome
- K48Ub-dependent proteasomal degradation of OsSPL14e0
- Direction
- promotese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which in turn promotes K48Ub-dependent proteasomal degradation of OsSPL14”
t0“rice”
Claim · model-written summary
Loss of OsOTUB1 function correlates with accumulation of OsSPL14 and new plant type architecture.
- Taxon
- riceOryza sativat0
- Material
- loss-of-function of OsOTUB1e0
- Gene
- OsOTUB1e0
- Outcome
- accumulation of high levels of OsSPL14e0
- Direction
- correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss-of-function of OsOTUB1 is correlated with the accumulation of high levels of OsSPL14, resulting in the NPT architecture”
t0“rice”
Claim · model-written summary
Pyramiding npt1 and dep1-1 alleles increased rice yield potential beyond the current achievable level.
- Taxon
- riceOryza sativae0
- Treatment
- pyramiding of high-yielding npt1 and dep1-1 allelese0
- Gene
- npt1 and dep1-1 allelese0
- Outcome
- rice yield potentiale0
- Direction
- increasinge0
- Comparator
- what is currently achievablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pyramiding of high-yielding npt1 and dep1-1 alleles provides a new strategy for increasing rice yield potential above what is currently achievable”
The same questions in other papers
loss of function · yield · Oryza sativa (rice)Positive and negative results
61 papers in the same direction as this paper · 72 in the opposite direction
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